Optimizing the cultivation of the cryptophyte Rhodomonas salina for aquaculture

Christina Vinum Thoisen

Optimizing the cultivation of the cryptophyte Rhodomonas salina for aquaculture

Christina Vinum Thoisen

Publikation: Bog/antologi/afhandling/rapport › Ph.d.-afhandling

Abstract

I akvakulturen kræver marinefiskearter af kommerciel interesse ofte levende
foder, når de er fiskelarver. Typisk anvendte levende foderorganismer er
hjuldyr og saltsøkrebs Artemia, selvom disse er suboptimale på grund af
utilfredsstillende størrelse og næringsværdi. Vandlopper er det naturlige bytte
for mange marine arter af fiskelarver, og mindsker dødeligheden og unormal
udvikling af fiskelarver. En stor skala produktion af vandloppen Acartia tonsa
til akvakulturen omfatter en storskalaproduktion af en mikroalge diæt til A.
tonsa. Flere undersøgelser viser, at cryptofytten Rhodomonas er en ideel
mikroalge diæt, da den har et optimalt næringsindhold, og øger overlevelse,
vækst og ægproduktion af vandlopper. Rhodomonas er dog en ret sensitiv
slægt, og optimering of kultiveringen er derfor nødvendig. Effekten af
forskellige kultiveringsparametre blev undersøgt på lille - og meso-skala
og inkluderede :
1) modifikation af vækstmediet, behandling af det anvendte vand til
kultivering og vækstraten af inokulum ved forskellige startkoncentrationer,
2) evaluering af anvendelse af kommercielle salte til kultivering,
3) evaluering af vækstrate og produktionsudbytte af densiteter på 5x105, 1x106, 1,5x106 og 2x106 celler ml -i en meso-skala tubular PBR,
4) evaluering af det biokemiske indhold af fem nært beslægtede Rhodomonas
-stammer ved temperaturer, der er relevante for dyrkning (15, 20 og 25 ̊C) for at bestemme, om der er en optimal temperatur og stamme, som er bedre
egnet til kultivering. Yderligere studier omfattede en evaluering af anvendelsen af den heterotrofe dinoflagellat Crypthecodinium cohnii som diæt til A. tonsa
sammenlignet med R. salina og udviklingen af en simpel og hurtig metode til
ekstraktion og kvantificering af pigmentet fycoerytrin fra Rhodomonas.

Originalsprog	Engelsk

Udgivelsessted	Roskilde
Forlag	Roskilde Universitet
Antal sider	161
Status	Udgivet - 2018

Note vedr. afhandling

Innovation Fund Denmark: COMA– Copepod egg Mass production in
Aquaculture (grant no. 67 - 2013 - 1)

Link til dokument

Christina Thoisen PhD Thesis 2018Forlagets udgivne version, 31,4 MB

Citer dette

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title = "Optimizing the cultivation of the cryptophyte Rhodomonas salina for aquaculture",

abstract = "In the aquaculture, marine fish species of commercial interest often require live feed at the first-feeding stage. Commonly used live feed organisms are rotifers and the brine shrimp Artemia although these are suboptimal due to inadequate size and nutritional value. Copepods are the natural prey of many marine species of fish larvae and decrease the mortality and abnormal development of fish larvae . A large-scale production of the copepod Acartia tonsa for aquaculture encompasses a large-scale production of a microalgal diet for A. tonsa. Several studies show that the cryptophyte Rhodomonas is an ideal microalgal diet as it has a superior nutritional content and increases the survival, growth and egg production of copepods. However, Rhodomonas is a rather sensitive genus and optimization of the cultivation is the refore required. The effects of various cultivation parameters were studied at small-and meso-scale and included:1) modification of the growth medium, treatment of the water used for cultivation, and the growth rate of inocula at different initial densities, 2) evaluation of the application of commercial salts for cultivation, 3)evaluation of growth rate and production yield of densities of 5x105; 1x106, 1.5x106 and 2x106 cells ml-1in a meso-scale tubular PBR, 4)evaluation of the biochemical content of five closely related strains of Rhodomonas at temperatures relevant for cultivation (15, 20 and 25 ̊C) to determine if there is an optimal temperature and strain more suitable for cultivation. Additional studies included an evaluation of using the heterotrophic dinoflagellate Crypthecodinium cohnii as a diet for A. tonsa compared to R.salina, and development of a simple and fast method for extraction and quantification of the pigment phycoerythrin from Rhodomonas.",

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N2 - In the aquaculture, marine fish species of commercial interest often require live feed at the first-feeding stage. Commonly used live feed organisms are rotifers and the brine shrimp Artemia although these are suboptimal due to inadequate size and nutritional value. Copepods are the natural prey of many marine species of fish larvae and decrease the mortality and abnormal development of fish larvae . A large-scale production of the copepod Acartia tonsa for aquaculture encompasses a large-scale production of a microalgal diet for A. tonsa. Several studies show that the cryptophyte Rhodomonas is an ideal microalgal diet as it has a superior nutritional content and increases the survival, growth and egg production of copepods. However, Rhodomonas is a rather sensitive genus and optimization of the cultivation is the refore required. The effects of various cultivation parameters were studied at small-and meso-scale and included:1) modification of the growth medium, treatment of the water used for cultivation, and the growth rate of inocula at different initial densities, 2) evaluation of the application of commercial salts for cultivation, 3)evaluation of growth rate and production yield of densities of 5x105; 1x106, 1.5x106 and 2x106 cells ml-1in a meso-scale tubular PBR, 4)evaluation of the biochemical content of five closely related strains of Rhodomonas at temperatures relevant for cultivation (15, 20 and 25 ̊C) to determine if there is an optimal temperature and strain more suitable for cultivation. Additional studies included an evaluation of using the heterotrophic dinoflagellate Crypthecodinium cohnii as a diet for A. tonsa compared to R.salina, and development of a simple and fast method for extraction and quantification of the pigment phycoerythrin from Rhodomonas.

AB - In the aquaculture, marine fish species of commercial interest often require live feed at the first-feeding stage. Commonly used live feed organisms are rotifers and the brine shrimp Artemia although these are suboptimal due to inadequate size and nutritional value. Copepods are the natural prey of many marine species of fish larvae and decrease the mortality and abnormal development of fish larvae . A large-scale production of the copepod Acartia tonsa for aquaculture encompasses a large-scale production of a microalgal diet for A. tonsa. Several studies show that the cryptophyte Rhodomonas is an ideal microalgal diet as it has a superior nutritional content and increases the survival, growth and egg production of copepods. However, Rhodomonas is a rather sensitive genus and optimization of the cultivation is the refore required. The effects of various cultivation parameters were studied at small-and meso-scale and included:1) modification of the growth medium, treatment of the water used for cultivation, and the growth rate of inocula at different initial densities, 2) evaluation of the application of commercial salts for cultivation, 3)evaluation of growth rate and production yield of densities of 5x105; 1x106, 1.5x106 and 2x106 cells ml-1in a meso-scale tubular PBR, 4)evaluation of the biochemical content of five closely related strains of Rhodomonas at temperatures relevant for cultivation (15, 20 and 25 ̊C) to determine if there is an optimal temperature and strain more suitable for cultivation. Additional studies included an evaluation of using the heterotrophic dinoflagellate Crypthecodinium cohnii as a diet for A. tonsa compared to R.salina, and development of a simple and fast method for extraction and quantification of the pigment phycoerythrin from Rhodomonas.

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