Novel multiplex format of an extended multilocus variable-number-tandem-repeat analysis of Clostridium difficile correlates with tandemrepeat sequence typing

Mie Birgitte Frid Jensen, Jørgen Engberg, Jonas T Larsson, Katharina E. P. Olsen, Mia Torpdahl

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstract

Subtyping of Clostridium difficile is crucial for outbreak investigations. An extended multilocus variable-number tandem-repeat analysis (eMLVA) of 14 variable number tandem repeat (VNTR) lociwas validated in multiplex format compatiblewith a routine typing laboratory and showed excellent concordance with tandem repeat se- quence typing (TRST) and high discriminatory power.
OriginalsprogEngelsk
TidsskriftJournal of Microbiological Methods
Vol/bind110
Sider (fra-til)98-101
Antal sider4
ISSN0167-7012
StatusUdgivet - mar. 2015
Udgivet eksterntJa

Emneord

  • Clostridium difficile
  • Molecular subtyping
  • Multilocus variable-number tandem-repeat analysis
  • Tandemrepeat sequence typing

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