Abstract
Humanmilk pepsin and pancreatin digests were separated using molecular membrane and reverse phase chromatography. Chemical screening of the resulting fractions using the ORAC antioxidant assay yielded a peptide fraction (PF-23) with high antioxidant activity (5207 μM Trolox Equivalents (TE)/g). Tandem mass spectrometry allowed the identification of twenty peptides. Eight small molecular weight peptides from 4 to 6 amino acids were synthesised and screened for antioxidant properties using ORAC and linoleic acid emulsion. On ORAC, the peptides YGYTGA (5169 μM TE/mmol) and ISELGW (4479 μM TE/mmol) were the most active. At 250 μM peptide ISELGW and its derivatives significantly reduced hydroperoxides formed during autoxidation of linoleic acid for 4 days at 50 °C. Further testing of these peptides may allow their inclusion in infant formulas to reduce the incidence of oxidative stress-mediated diseases in newborns.
Originalsprog | Engelsk |
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Tidsskrift | Food Chemistry |
Vol/bind | 126 |
Udgave nummer | 3 |
Sider (fra-til) | 1138-43 |
ISSN | 0308-8146 |
DOI | |
Status | Udgivet - 2011 |