Novel anti-oxidative peptides from enzymatic digestion of human milk

Apollinaire Tsopmo, Andrea Romanowski, Lyness Banda, Jean Claude Lavoie, Håvard Jenssen, James K. Friel

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Humanmilk pepsin and pancreatin digests were separated using molecular membrane and reverse phase chromatography. Chemical screening of the resulting fractions using the ORAC antioxidant assay yielded a peptide fraction (PF-23) with high antioxidant activity (5207 μM Trolox Equivalents (TE)/g). Tandem mass spectrometry allowed the identification of twenty peptides. Eight small molecular weight peptides from 4 to 6 amino acids were synthesised and screened for antioxidant properties using ORAC and linoleic acid emulsion. On ORAC, the peptides YGYTGA (5169 μM TE/mmol) and ISELGW (4479 μM TE/mmol) were the most active. At 250 μM peptide ISELGW and its derivatives significantly reduced hydroperoxides formed during autoxidation of linoleic acid for 4 days at 50 °C. Further testing of these peptides may allow their inclusion in infant formulas to reduce the incidence of oxidative stress-mediated diseases in newborns.
    OriginalsprogEngelsk
    TidsskriftFood Chemistry
    Vol/bind126
    Udgave nummer3
    Sider (fra-til)1138-43
    ISSN0308-8146
    DOI
    StatusUdgivet - 2011

    Citer dette

    Tsopmo, A., Romanowski, A., Banda, L., Lavoie, J. C., Jenssen, H., & Friel, J. K. (2011). Novel anti-oxidative peptides from enzymatic digestion of human milk. Food Chemistry, 126(3), 1138-43. https://doi.org/10.1016/j.foodchem.2010.11.146
    Tsopmo, Apollinaire ; Romanowski, Andrea ; Banda, Lyness ; Lavoie, Jean Claude ; Jenssen, Håvard ; Friel, James K. / Novel anti-oxidative peptides from enzymatic digestion of human milk. I: Food Chemistry. 2011 ; Bind 126, Nr. 3. s. 1138-43.
    @article{6b0eb3d87a22482eb8ec8c2af9e3be8e,
    title = "Novel anti-oxidative peptides from enzymatic digestion of human milk",
    abstract = "Humanmilk pepsin and pancreatin digests were separated using molecular membrane and reverse phase chromatography. Chemical screening of the resulting fractions using the ORAC antioxidant assay yielded a peptide fraction (PF-23) with high antioxidant activity (5207 μM Trolox Equivalents (TE)/g). Tandem mass spectrometry allowed the identification of twenty peptides. Eight small molecular weight peptides from 4 to 6 amino acids were synthesised and screened for antioxidant properties using ORAC and linoleic acid emulsion. On ORAC, the peptides YGYTGA (5169 μM TE/mmol) and ISELGW (4479 μM TE/mmol) were the most active. At 250 μM peptide ISELGW and its derivatives significantly reduced hydroperoxides formed during autoxidation of linoleic acid for 4 days at 50 °C. Further testing of these peptides may allow their inclusion in infant formulas to reduce the incidence of oxidative stress-mediated diseases in newborns.",
    keywords = "Humanmilk, Peptide, Antioxidant, Hydroperoxides, Premature infant, Oxidative stress, Oxygen radical absorbance capacity",
    author = "Apollinaire Tsopmo and Andrea Romanowski and Lyness Banda and Lavoie, {Jean Claude} and H{\aa}vard Jenssen and Friel, {James K.}",
    year = "2011",
    doi = "10.1016/j.foodchem.2010.11.146",
    language = "English",
    volume = "126",
    pages = "1138--43",
    journal = "Food Chemistry",
    issn = "0308-8146",
    publisher = "Elsevier BV",
    number = "3",

    }

    Tsopmo, A, Romanowski, A, Banda, L, Lavoie, JC, Jenssen, H & Friel, JK 2011, 'Novel anti-oxidative peptides from enzymatic digestion of human milk', Food Chemistry, bind 126, nr. 3, s. 1138-43. https://doi.org/10.1016/j.foodchem.2010.11.146

    Novel anti-oxidative peptides from enzymatic digestion of human milk. / Tsopmo, Apollinaire ; Romanowski, Andrea; Banda, Lyness; Lavoie, Jean Claude; Jenssen, Håvard; Friel, James K.

    I: Food Chemistry, Bind 126, Nr. 3, 2011, s. 1138-43.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Novel anti-oxidative peptides from enzymatic digestion of human milk

    AU - Tsopmo, Apollinaire

    AU - Romanowski, Andrea

    AU - Banda, Lyness

    AU - Lavoie, Jean Claude

    AU - Jenssen, Håvard

    AU - Friel, James K.

    PY - 2011

    Y1 - 2011

    N2 - Humanmilk pepsin and pancreatin digests were separated using molecular membrane and reverse phase chromatography. Chemical screening of the resulting fractions using the ORAC antioxidant assay yielded a peptide fraction (PF-23) with high antioxidant activity (5207 μM Trolox Equivalents (TE)/g). Tandem mass spectrometry allowed the identification of twenty peptides. Eight small molecular weight peptides from 4 to 6 amino acids were synthesised and screened for antioxidant properties using ORAC and linoleic acid emulsion. On ORAC, the peptides YGYTGA (5169 μM TE/mmol) and ISELGW (4479 μM TE/mmol) were the most active. At 250 μM peptide ISELGW and its derivatives significantly reduced hydroperoxides formed during autoxidation of linoleic acid for 4 days at 50 °C. Further testing of these peptides may allow their inclusion in infant formulas to reduce the incidence of oxidative stress-mediated diseases in newborns.

    AB - Humanmilk pepsin and pancreatin digests were separated using molecular membrane and reverse phase chromatography. Chemical screening of the resulting fractions using the ORAC antioxidant assay yielded a peptide fraction (PF-23) with high antioxidant activity (5207 μM Trolox Equivalents (TE)/g). Tandem mass spectrometry allowed the identification of twenty peptides. Eight small molecular weight peptides from 4 to 6 amino acids were synthesised and screened for antioxidant properties using ORAC and linoleic acid emulsion. On ORAC, the peptides YGYTGA (5169 μM TE/mmol) and ISELGW (4479 μM TE/mmol) were the most active. At 250 μM peptide ISELGW and its derivatives significantly reduced hydroperoxides formed during autoxidation of linoleic acid for 4 days at 50 °C. Further testing of these peptides may allow their inclusion in infant formulas to reduce the incidence of oxidative stress-mediated diseases in newborns.

    KW - Humanmilk

    KW - Peptide

    KW - Antioxidant

    KW - Hydroperoxides

    KW - Premature infant

    KW - Oxidative stress

    KW - Oxygen radical absorbance capacity

    U2 - 10.1016/j.foodchem.2010.11.146

    DO - 10.1016/j.foodchem.2010.11.146

    M3 - Journal article

    VL - 126

    SP - 1138

    EP - 1143

    JO - Food Chemistry

    JF - Food Chemistry

    SN - 0308-8146

    IS - 3

    ER -