No Cytotoxicity or Genotoxicity of Graphene and Graphene Oxide in Murine Lung Epithelial FE1 Cells in Vitro

Stefan Bengtson, Kirsten Kling, Anne Mette Madsen, Asger Nørgaard, Nicklas Raun Jacobsen, Per Axel Clausen, Beatriz Alonso, Amaia Pesquera, Amaia Zurutuza, Raphael Ramos, Hanako Okuno, Jean Dijon, Håkan Wallin, Ulla Vogel

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Graphene and graphene oxide receive much attention these years, because they add attrac- tive properties to a wide range of applications and products. Several studies have shown toxi- cological effects of other carbon-based nanoma- terials such as carbon black nanoparticles and carbon nanotubes in vitro and in vivo. Here, we report in-depth physicochemical characteriza- tion of three commercial graphene materials, one graphene oxide (GO) and two reduced graphene oxides (rGO) and assess cytotoxicity and genotoxicity in the murine lung epithelial cell line FE1. The studied GO and rGO mainly consisted of 2–3 graphene layers with lateral sizes of 1–2 mm. GO had almost equimolar content of C, O, and H while the two rGO materials had lower contents of oxygen with C/O and C/H ratios of 8 and 12.8, respectively. All materials had low levels of endotoxin and low levels of inorganic impurities, which were mainly sulphur, manganese, and silicon. GO generated more ROS than the two rGO materi- als, but none of the graphene materials influ- enced cytotoxicity in terms of cell viability and cell proliferation after 24 hr. Furthermore, no genotoxicity was observed using the alkaline comet assay following 3 or 24 hr of exposure. We demonstrate that chemically pure, few- layered GO and rGO with comparable lateral size (> 1 mm) do not induce significant cytotox- icity or genotoxicity in FE1 cells at relatively high doses (5–200 mg/ml).
OriginalsprogEngelsk
TidsskriftEnvironmental and Molecular Mutagenesis
Vol/bind57
Udgave nummer6
Sider (fra-til)469–482
ISSN0893-6692
DOI
StatusUdgivet - 2016

Citer dette

Bengtson, S., Kling, K., Madsen, A. M., Nørgaard, A., Raun Jacobsen, N., Clausen, P. A., ... Vogel, U. (2016). No Cytotoxicity or Genotoxicity of Graphene and Graphene Oxide in Murine Lung Epithelial FE1 Cells in Vitro. Environmental and Molecular Mutagenesis, 57(6), 469–482. https://doi.org/10.1002/em.22017
Bengtson, Stefan ; Kling, Kirsten ; Madsen, Anne Mette ; Nørgaard, Asger ; Raun Jacobsen, Nicklas ; Clausen, Per Axel ; Alonso, Beatriz ; Pesquera, Amaia ; Zurutuza, Amaia ; Ramos, Raphael ; Okuno, Hanako ; Dijon, Jean ; Wallin, Håkan ; Vogel, Ulla. / No Cytotoxicity or Genotoxicity of Graphene and Graphene Oxide in Murine Lung Epithelial FE1 Cells in Vitro. I: Environmental and Molecular Mutagenesis. 2016 ; Bind 57, Nr. 6. s. 469–482.
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title = "No Cytotoxicity or Genotoxicity of Graphene and Graphene Oxide in Murine Lung Epithelial FE1 Cells in Vitro",
abstract = "Graphene and graphene oxide receive much attention these years, because they add attrac- tive properties to a wide range of applications and products. Several studies have shown toxi- cological effects of other carbon-based nanoma- terials such as carbon black nanoparticles and carbon nanotubes in vitro and in vivo. Here, we report in-depth physicochemical characteriza- tion of three commercial graphene materials, one graphene oxide (GO) and two reduced graphene oxides (rGO) and assess cytotoxicity and genotoxicity in the murine lung epithelial cell line FE1. The studied GO and rGO mainly consisted of 2–3 graphene layers with lateral sizes of 1–2 mm. GO had almost equimolar content of C, O, and H while the two rGO materials had lower contents of oxygen with C/O and C/H ratios of 8 and 12.8, respectively. All materials had low levels of endotoxin and low levels of inorganic impurities, which were mainly sulphur, manganese, and silicon. GO generated more ROS than the two rGO materi- als, but none of the graphene materials influ- enced cytotoxicity in terms of cell viability and cell proliferation after 24 hr. Furthermore, no genotoxicity was observed using the alkaline comet assay following 3 or 24 hr of exposure. We demonstrate that chemically pure, few- layered GO and rGO with comparable lateral size (> 1 mm) do not induce significant cytotox- icity or genotoxicity in FE1 cells at relatively high doses (5–200 mg/ml).",
author = "Stefan Bengtson and Kirsten Kling and Madsen, {Anne Mette} and Asger N{\o}rgaard and {Raun Jacobsen}, Nicklas and Clausen, {Per Axel} and Beatriz Alonso and Amaia Pesquera and Amaia Zurutuza and Raphael Ramos and Hanako Okuno and Jean Dijon and H{\aa}kan Wallin and Ulla Vogel",
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Bengtson, S, Kling, K, Madsen, AM, Nørgaard, A, Raun Jacobsen, N, Clausen, PA, Alonso, B, Pesquera, A, Zurutuza, A, Ramos, R, Okuno, H, Dijon, J, Wallin, H & Vogel, U 2016, 'No Cytotoxicity or Genotoxicity of Graphene and Graphene Oxide in Murine Lung Epithelial FE1 Cells in Vitro', Environmental and Molecular Mutagenesis, bind 57, nr. 6, s. 469–482. https://doi.org/10.1002/em.22017

No Cytotoxicity or Genotoxicity of Graphene and Graphene Oxide in Murine Lung Epithelial FE1 Cells in Vitro. / Bengtson, Stefan; Kling, Kirsten; Madsen, Anne Mette; Nørgaard, Asger ; Raun Jacobsen, Nicklas; Clausen, Per Axel; Alonso, Beatriz; Pesquera, Amaia; Zurutuza, Amaia; Ramos, Raphael; Okuno, Hanako; Dijon, Jean; Wallin, Håkan; Vogel, Ulla.

I: Environmental and Molecular Mutagenesis, Bind 57, Nr. 6, 2016, s. 469–482.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - No Cytotoxicity or Genotoxicity of Graphene and Graphene Oxide in Murine Lung Epithelial FE1 Cells in Vitro

AU - Bengtson, Stefan

AU - Kling, Kirsten

AU - Madsen, Anne Mette

AU - Nørgaard, Asger

AU - Raun Jacobsen, Nicklas

AU - Clausen, Per Axel

AU - Alonso, Beatriz

AU - Pesquera, Amaia

AU - Zurutuza, Amaia

AU - Ramos, Raphael

AU - Okuno, Hanako

AU - Dijon, Jean

AU - Wallin, Håkan

AU - Vogel, Ulla

PY - 2016

Y1 - 2016

N2 - Graphene and graphene oxide receive much attention these years, because they add attrac- tive properties to a wide range of applications and products. Several studies have shown toxi- cological effects of other carbon-based nanoma- terials such as carbon black nanoparticles and carbon nanotubes in vitro and in vivo. Here, we report in-depth physicochemical characteriza- tion of three commercial graphene materials, one graphene oxide (GO) and two reduced graphene oxides (rGO) and assess cytotoxicity and genotoxicity in the murine lung epithelial cell line FE1. The studied GO and rGO mainly consisted of 2–3 graphene layers with lateral sizes of 1–2 mm. GO had almost equimolar content of C, O, and H while the two rGO materials had lower contents of oxygen with C/O and C/H ratios of 8 and 12.8, respectively. All materials had low levels of endotoxin and low levels of inorganic impurities, which were mainly sulphur, manganese, and silicon. GO generated more ROS than the two rGO materi- als, but none of the graphene materials influ- enced cytotoxicity in terms of cell viability and cell proliferation after 24 hr. Furthermore, no genotoxicity was observed using the alkaline comet assay following 3 or 24 hr of exposure. We demonstrate that chemically pure, few- layered GO and rGO with comparable lateral size (> 1 mm) do not induce significant cytotox- icity or genotoxicity in FE1 cells at relatively high doses (5–200 mg/ml).

AB - Graphene and graphene oxide receive much attention these years, because they add attrac- tive properties to a wide range of applications and products. Several studies have shown toxi- cological effects of other carbon-based nanoma- terials such as carbon black nanoparticles and carbon nanotubes in vitro and in vivo. Here, we report in-depth physicochemical characteriza- tion of three commercial graphene materials, one graphene oxide (GO) and two reduced graphene oxides (rGO) and assess cytotoxicity and genotoxicity in the murine lung epithelial cell line FE1. The studied GO and rGO mainly consisted of 2–3 graphene layers with lateral sizes of 1–2 mm. GO had almost equimolar content of C, O, and H while the two rGO materials had lower contents of oxygen with C/O and C/H ratios of 8 and 12.8, respectively. All materials had low levels of endotoxin and low levels of inorganic impurities, which were mainly sulphur, manganese, and silicon. GO generated more ROS than the two rGO materi- als, but none of the graphene materials influ- enced cytotoxicity in terms of cell viability and cell proliferation after 24 hr. Furthermore, no genotoxicity was observed using the alkaline comet assay following 3 or 24 hr of exposure. We demonstrate that chemically pure, few- layered GO and rGO with comparable lateral size (> 1 mm) do not induce significant cytotox- icity or genotoxicity in FE1 cells at relatively high doses (5–200 mg/ml).

U2 - 10.1002/em.22017

DO - 10.1002/em.22017

M3 - Journal article

VL - 57

SP - 469

EP - 482

JO - Environmental and Molecular Mutagenesis

JF - Environmental and Molecular Mutagenesis

SN - 0893-6692

IS - 6

ER -