NMR-based metabonomics reveals that plasma betaine increases upon intake of high-fiber rye buns in hypercholesterolemic pigs

Hanne Christine S. Bertram, Anders Malmendal, Niels Chr Nielsen, Ida Krestine Straadt, Torben Larsen, Knud Erik Bach Knudsen, Helle Nygaard Laerke

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

This study presents an NMR-based metabonomic approach to explore the overall endogenous biochemical effects of a rye versus wheat-based fiber-rich diet in hypercholesterolemic pigs. The pigs were fed high-fat, high-cholesterol rye- (n = 9) or wheat- (n = 8) based buns with similar levels of dietary fiber for 9-10 wk. Fasting plasma samples were collected 2 days before and after 8 and 12 days on the experimental diets, while postprandial samples taken after 58-67 days, and( 1)H NMR spectra were acquired on these. Principal component analysis on the obtained NMR spectra demonstrated clear effects of diet on the plasma metabolite profile, and partial least squares regression discriminant analysis on the spectra revealed that the intensity of the spectral region at 3.29 ppm dominated the differentiation between the two diets, as the rye diet was associated with higher spectral intensity in this region. The 3.29 ppm signal is ascribed to N(CH(3))(3) protons in betaine, which may be an important contributor to the health promoting effects of rye.
OriginalsprogEngelsk
TidsskriftMolecular Nutrition & Food Research (Online)
Vol/bind53
Udgave nummer8
Sider (fra-til)1055-62
Antal sider8
ISSN1613-4133
DOI
StatusUdgivet - 2009
Udgivet eksterntJa

Citer dette

Bertram, Hanne Christine S. ; Malmendal, Anders ; Nielsen, Niels Chr ; Straadt, Ida Krestine ; Larsen, Torben ; Knudsen, Knud Erik Bach ; Laerke, Helle Nygaard. / NMR-based metabonomics reveals that plasma betaine increases upon intake of high-fiber rye buns in hypercholesterolemic pigs. I: Molecular Nutrition & Food Research (Online). 2009 ; Bind 53, Nr. 8. s. 1055-62.
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title = "NMR-based metabonomics reveals that plasma betaine increases upon intake of high-fiber rye buns in hypercholesterolemic pigs",
abstract = "This study presents an NMR-based metabonomic approach to explore the overall endogenous biochemical effects of a rye versus wheat-based fiber-rich diet in hypercholesterolemic pigs. The pigs were fed high-fat, high-cholesterol rye- (n = 9) or wheat- (n = 8) based buns with similar levels of dietary fiber for 9-10 wk. Fasting plasma samples were collected 2 days before and after 8 and 12 days on the experimental diets, while postprandial samples taken after 58-67 days, and( 1)H NMR spectra were acquired on these. Principal component analysis on the obtained NMR spectra demonstrated clear effects of diet on the plasma metabolite profile, and partial least squares regression discriminant analysis on the spectra revealed that the intensity of the spectral region at 3.29 ppm dominated the differentiation between the two diets, as the rye diet was associated with higher spectral intensity in this region. The 3.29 ppm signal is ascribed to N(CH(3))(3) protons in betaine, which may be an important contributor to the health promoting effects of rye.",
author = "Bertram, {Hanne Christine S.} and Anders Malmendal and Nielsen, {Niels Chr} and Straadt, {Ida Krestine} and Torben Larsen and Knudsen, {Knud Erik Bach} and Laerke, {Helle Nygaard}",
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NMR-based metabonomics reveals that plasma betaine increases upon intake of high-fiber rye buns in hypercholesterolemic pigs. / Bertram, Hanne Christine S.; Malmendal, Anders; Nielsen, Niels Chr; Straadt, Ida Krestine; Larsen, Torben; Knudsen, Knud Erik Bach; Laerke, Helle Nygaard.

I: Molecular Nutrition & Food Research (Online), Bind 53, Nr. 8, 2009, s. 1055-62.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - NMR-based metabonomics reveals that plasma betaine increases upon intake of high-fiber rye buns in hypercholesterolemic pigs

AU - Bertram, Hanne Christine S.

AU - Malmendal, Anders

AU - Nielsen, Niels Chr

AU - Straadt, Ida Krestine

AU - Larsen, Torben

AU - Knudsen, Knud Erik Bach

AU - Laerke, Helle Nygaard

PY - 2009

Y1 - 2009

N2 - This study presents an NMR-based metabonomic approach to explore the overall endogenous biochemical effects of a rye versus wheat-based fiber-rich diet in hypercholesterolemic pigs. The pigs were fed high-fat, high-cholesterol rye- (n = 9) or wheat- (n = 8) based buns with similar levels of dietary fiber for 9-10 wk. Fasting plasma samples were collected 2 days before and after 8 and 12 days on the experimental diets, while postprandial samples taken after 58-67 days, and( 1)H NMR spectra were acquired on these. Principal component analysis on the obtained NMR spectra demonstrated clear effects of diet on the plasma metabolite profile, and partial least squares regression discriminant analysis on the spectra revealed that the intensity of the spectral region at 3.29 ppm dominated the differentiation between the two diets, as the rye diet was associated with higher spectral intensity in this region. The 3.29 ppm signal is ascribed to N(CH(3))(3) protons in betaine, which may be an important contributor to the health promoting effects of rye.

AB - This study presents an NMR-based metabonomic approach to explore the overall endogenous biochemical effects of a rye versus wheat-based fiber-rich diet in hypercholesterolemic pigs. The pigs were fed high-fat, high-cholesterol rye- (n = 9) or wheat- (n = 8) based buns with similar levels of dietary fiber for 9-10 wk. Fasting plasma samples were collected 2 days before and after 8 and 12 days on the experimental diets, while postprandial samples taken after 58-67 days, and( 1)H NMR spectra were acquired on these. Principal component analysis on the obtained NMR spectra demonstrated clear effects of diet on the plasma metabolite profile, and partial least squares regression discriminant analysis on the spectra revealed that the intensity of the spectral region at 3.29 ppm dominated the differentiation between the two diets, as the rye diet was associated with higher spectral intensity in this region. The 3.29 ppm signal is ascribed to N(CH(3))(3) protons in betaine, which may be an important contributor to the health promoting effects of rye.

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DO - 10.1002/mnfr.200800344

M3 - Journal article

VL - 53

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JO - Molecular Nutrition & Food Research (Online)

JF - Molecular Nutrition & Food Research (Online)

SN - 1613-4133

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