Mutation Spectrum in FE1-Muta (TM) Mouse Lung Epithelial Cells Exposed to Nanoparticulate Carbon Black

Nicklas Raun Jacobsen, Paul A. White, John Gingerich, Peter Møller, Anne Thoustrup Saber, George R. Douglas, Ulla Vogel, Håkan Wallin

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    It has been shown previously that carbon black (CB), Printex 90 exposure induces cII and lacZ mutants in the FE1-Muta (TM) Mouse lung epithelial cell line and causes oxidatively damaged DNA and the production of reactive oxygen species (ROS). The purpose of this study was to determine the mutation spectrum in the cII gene of Printex 90 exposed cells. Cells exposed to CB have a substantially different mutation spectrum in the cII gene compared with vehicle exposed controls. The mutation spectra differ both in the positions (P < 0.0001) and types of the mutations (P < 0.0001). Exposure to Printex 90 increased the number of single base deletions by 2.3-fold and larger deletions by 1.9-fold. Most single base deletions were within two repetitive sequences in cII, but the large deletions were not. The mechanism behind the large deletions is not yet known. The largest increases in base substitutions were observed in G:C -> T:A, G:C -> C:G, and A:T -> T:A transversion mutations; this is in keeping with a genetic finger print of ROS and is further substantiated by the observations that Printex 90 generates ROS and oxidatively damaged DNA
    OriginalsprogEngelsk
    TidsskriftEnvironmental and Molecular Mutagenesis
    Vol/bind52
    Udgave nummer4
    Sider (fra-til)331-337
    ISSN0893-6692
    DOI
    StatusUdgivet - 2011

    Citer dette

    Jacobsen, N. R., White, P. A., Gingerich, J., Møller, P., Saber, A. T., Douglas, G. R., ... Wallin, H. (2011). Mutation Spectrum in FE1-Muta (TM) Mouse Lung Epithelial Cells Exposed to Nanoparticulate Carbon Black. Environmental and Molecular Mutagenesis, 52(4), 331-337 . https://doi.org/10.1002/em.20629
    Jacobsen, Nicklas Raun ; White, Paul A. ; Gingerich, John ; Møller, Peter ; Saber, Anne Thoustrup ; Douglas, George R. ; Vogel, Ulla ; Wallin, Håkan. / Mutation Spectrum in FE1-Muta (TM) Mouse Lung Epithelial Cells Exposed to Nanoparticulate Carbon Black. I: Environmental and Molecular Mutagenesis. 2011 ; Bind 52, Nr. 4. s. 331-337 .
    @article{fa53fb66b7164e3d90e7a5e42962b4d5,
    title = "Mutation Spectrum in FE1-Muta (TM) Mouse Lung Epithelial Cells Exposed to Nanoparticulate Carbon Black",
    abstract = "It has been shown previously that carbon black (CB), Printex 90 exposure induces cII and lacZ mutants in the FE1-Muta (TM) Mouse lung epithelial cell line and causes oxidatively damaged DNA and the production of reactive oxygen species (ROS). The purpose of this study was to determine the mutation spectrum in the cII gene of Printex 90 exposed cells. Cells exposed to CB have a substantially different mutation spectrum in the cII gene compared with vehicle exposed controls. The mutation spectra differ both in the positions (P < 0.0001) and types of the mutations (P < 0.0001). Exposure to Printex 90 increased the number of single base deletions by 2.3-fold and larger deletions by 1.9-fold. Most single base deletions were within two repetitive sequences in cII, but the large deletions were not. The mechanism behind the large deletions is not yet known. The largest increases in base substitutions were observed in G:C -> T:A, G:C -> C:G, and A:T -> T:A transversion mutations; this is in keeping with a genetic finger print of ROS and is further substantiated by the observations that Printex 90 generates ROS and oxidatively damaged DNA",
    keywords = "deletions, mutation spectrum analysis, transversion mutations, oxidative damage",
    author = "Jacobsen, {Nicklas Raun} and White, {Paul A.} and John Gingerich and Peter M{\o}ller and Saber, {Anne Thoustrup} and Douglas, {George R.} and Ulla Vogel and H{\aa}kan Wallin",
    year = "2011",
    doi = "10.1002/em.20629",
    language = "English",
    volume = "52",
    pages = "331--337",
    journal = "Environmental and Molecular Mutagenesis",
    issn = "0893-6692",
    publisher = "John/Wiley & Sons, Inc. John/Wiley & Sons Ltd.",
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    Jacobsen, NR, White, PA, Gingerich, J, Møller, P, Saber, AT, Douglas, GR, Vogel, U & Wallin, H 2011, 'Mutation Spectrum in FE1-Muta (TM) Mouse Lung Epithelial Cells Exposed to Nanoparticulate Carbon Black', Environmental and Molecular Mutagenesis, bind 52, nr. 4, s. 331-337 . https://doi.org/10.1002/em.20629

    Mutation Spectrum in FE1-Muta (TM) Mouse Lung Epithelial Cells Exposed to Nanoparticulate Carbon Black. / Jacobsen, Nicklas Raun; White, Paul A. ; Gingerich, John; Møller, Peter; Saber, Anne Thoustrup; Douglas, George R.; Vogel, Ulla; Wallin, Håkan.

    I: Environmental and Molecular Mutagenesis, Bind 52, Nr. 4, 2011, s. 331-337 .

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Mutation Spectrum in FE1-Muta (TM) Mouse Lung Epithelial Cells Exposed to Nanoparticulate Carbon Black

    AU - Jacobsen, Nicklas Raun

    AU - White, Paul A.

    AU - Gingerich, John

    AU - Møller, Peter

    AU - Saber, Anne Thoustrup

    AU - Douglas, George R.

    AU - Vogel, Ulla

    AU - Wallin, Håkan

    PY - 2011

    Y1 - 2011

    N2 - It has been shown previously that carbon black (CB), Printex 90 exposure induces cII and lacZ mutants in the FE1-Muta (TM) Mouse lung epithelial cell line and causes oxidatively damaged DNA and the production of reactive oxygen species (ROS). The purpose of this study was to determine the mutation spectrum in the cII gene of Printex 90 exposed cells. Cells exposed to CB have a substantially different mutation spectrum in the cII gene compared with vehicle exposed controls. The mutation spectra differ both in the positions (P < 0.0001) and types of the mutations (P < 0.0001). Exposure to Printex 90 increased the number of single base deletions by 2.3-fold and larger deletions by 1.9-fold. Most single base deletions were within two repetitive sequences in cII, but the large deletions were not. The mechanism behind the large deletions is not yet known. The largest increases in base substitutions were observed in G:C -> T:A, G:C -> C:G, and A:T -> T:A transversion mutations; this is in keeping with a genetic finger print of ROS and is further substantiated by the observations that Printex 90 generates ROS and oxidatively damaged DNA

    AB - It has been shown previously that carbon black (CB), Printex 90 exposure induces cII and lacZ mutants in the FE1-Muta (TM) Mouse lung epithelial cell line and causes oxidatively damaged DNA and the production of reactive oxygen species (ROS). The purpose of this study was to determine the mutation spectrum in the cII gene of Printex 90 exposed cells. Cells exposed to CB have a substantially different mutation spectrum in the cII gene compared with vehicle exposed controls. The mutation spectra differ both in the positions (P < 0.0001) and types of the mutations (P < 0.0001). Exposure to Printex 90 increased the number of single base deletions by 2.3-fold and larger deletions by 1.9-fold. Most single base deletions were within two repetitive sequences in cII, but the large deletions were not. The mechanism behind the large deletions is not yet known. The largest increases in base substitutions were observed in G:C -> T:A, G:C -> C:G, and A:T -> T:A transversion mutations; this is in keeping with a genetic finger print of ROS and is further substantiated by the observations that Printex 90 generates ROS and oxidatively damaged DNA

    KW - deletions

    KW - mutation spectrum analysis

    KW - transversion mutations

    KW - oxidative damage

    U2 - 10.1002/em.20629

    DO - 10.1002/em.20629

    M3 - Journal article

    VL - 52

    SP - 331

    EP - 337

    JO - Environmental and Molecular Mutagenesis

    JF - Environmental and Molecular Mutagenesis

    SN - 0893-6692

    IS - 4

    ER -