Multilocus sequence typing and biocide tolerance of Arcobacter butzleri from Danish broiler carcasses

Louise Hesselbjerg Rasmussen, Jette Kjeldgaard, Jens Peter Christensen, Hanne Ingmer

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Background
    Arcobacter spp. have in recent years received increasing interest as potential emerging enteropathogens and zoonotic agents. They are associated with various animals including poultry and can be isolated from meat products. The possibilities of persistence and cross-contamination in slaughterhouses during meat processing are not well established. We have evaluated the occurrence and persistence of Arcobacter spp. in a Danish slaughterhouse and determined the sensitivity of isolates to sodium hypochlorite, a commonly used biocide.
    Results
    Arcobacter contamination was examined in a broiler slaughterhouse by selective enrichment of 235 swabs from the processing line during two production days and after sanitizing in between. In total 13.6% of samples were positive for A. butzleri with the majority (29 of 32 isolates) originating from the evisceration machine. No Arcobacter spp. was isolated after cleaning. A. butzleri isolates confirmed by PCR were typed by multilocus sequence typing (MLST) resulting in 10 new sequence types (STs). Two sequence types were isolated on both processing days. Minimum inhibitory concentration (MIC) to sodium hypochlorite was determined to 0.5% hypochlorite biocide (500 ppm chlorine) for most isolates, which allows growth of A. butzleri within the working concentration of the biocide (0.2 - 0.5%).
    Conclusions
    A. butzleri was readily isolated from a Danish broiler slaughterhouse, primarily in the evisceration machine. Typing by MLST showed high strain variability but the recurrence of two STs indicate that some persistence or cross-contamination takes place. Importantly, the isolates tolerated sodium hypochlorite, a biocide commonly employed in slaughterhouse sanitizing, at levels close to the disinfection concentration, and thus, A. butzleri may survive the disinfection process although this was not observed in our study.
    OriginalsprogEngelsk
    TidsskriftBMC Research Notes
    Vol/bind6
    Udgave nummer322
    ISSN1756-0500
    DOI
    StatusUdgivet - 2013

    Emneord

    • Arcobacter butzleri
    • MLST
    • Chicken slaughterhouse
    • MIC
    • Sodium hypochlorite

    Citer dette

    Rasmussen, Louise Hesselbjerg ; Kjeldgaard, Jette ; Christensen, Jens Peter ; Ingmer, Hanne. / Multilocus sequence typing and biocide tolerance of Arcobacter butzleri from Danish broiler carcasses. I: BMC Research Notes. 2013 ; Bind 6, Nr. 322.
    @article{5de0afca541642a0b1e0439711f6a42d,
    title = "Multilocus sequence typing and biocide tolerance of Arcobacter butzleri from Danish broiler carcasses",
    abstract = "BackgroundArcobacter spp. have in recent years received increasing interest as potential emerging enteropathogens and zoonotic agents. They are associated with various animals including poultry and can be isolated from meat products. The possibilities of persistence and cross-contamination in slaughterhouses during meat processing are not well established. We have evaluated the occurrence and persistence of Arcobacter spp. in a Danish slaughterhouse and determined the sensitivity of isolates to sodium hypochlorite, a commonly used biocide. ResultsArcobacter contamination was examined in a broiler slaughterhouse by selective enrichment of 235 swabs from the processing line during two production days and after sanitizing in between. In total 13.6{\%} of samples were positive for A. butzleri with the majority (29 of 32 isolates) originating from the evisceration machine. No Arcobacter spp. was isolated after cleaning. A. butzleri isolates confirmed by PCR were typed by multilocus sequence typing (MLST) resulting in 10 new sequence types (STs). Two sequence types were isolated on both processing days. Minimum inhibitory concentration (MIC) to sodium hypochlorite was determined to 0.5{\%} hypochlorite biocide (500 ppm chlorine) for most isolates, which allows growth of A. butzleri within the working concentration of the biocide (0.2 - 0.5{\%}). ConclusionsA. butzleri was readily isolated from a Danish broiler slaughterhouse, primarily in the evisceration machine. Typing by MLST showed high strain variability but the recurrence of two STs indicate that some persistence or cross-contamination takes place. Importantly, the isolates tolerated sodium hypochlorite, a biocide commonly employed in slaughterhouse sanitizing, at levels close to the disinfection concentration, and thus, A. butzleri may survive the disinfection process although this was not observed in our study.",
    keywords = "Arcobacter butzleri, MLST, Chicken slaughterhouse, MIC, Sodium hypochlorite, Arcobacter butzleri, MLST, Chicken slaughterhouse, MIC, Sodium hypochlorite",
    author = "Rasmussen, {Louise Hesselbjerg} and Jette Kjeldgaard and Christensen, {Jens Peter} and Hanne Ingmer",
    year = "2013",
    doi = "10.1186/1756-0500-6-322",
    language = "English",
    volume = "6",
    journal = "BMC Research Notes",
    issn = "1756-0500",
    publisher = "BioMed Central Ltd.",
    number = "322",

    }

    Multilocus sequence typing and biocide tolerance of Arcobacter butzleri from Danish broiler carcasses. / Rasmussen, Louise Hesselbjerg; Kjeldgaard, Jette; Christensen, Jens Peter; Ingmer, Hanne.

    I: BMC Research Notes, Bind 6, Nr. 322, 2013.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Multilocus sequence typing and biocide tolerance of Arcobacter butzleri from Danish broiler carcasses

    AU - Rasmussen, Louise Hesselbjerg

    AU - Kjeldgaard, Jette

    AU - Christensen, Jens Peter

    AU - Ingmer, Hanne

    PY - 2013

    Y1 - 2013

    N2 - BackgroundArcobacter spp. have in recent years received increasing interest as potential emerging enteropathogens and zoonotic agents. They are associated with various animals including poultry and can be isolated from meat products. The possibilities of persistence and cross-contamination in slaughterhouses during meat processing are not well established. We have evaluated the occurrence and persistence of Arcobacter spp. in a Danish slaughterhouse and determined the sensitivity of isolates to sodium hypochlorite, a commonly used biocide. ResultsArcobacter contamination was examined in a broiler slaughterhouse by selective enrichment of 235 swabs from the processing line during two production days and after sanitizing in between. In total 13.6% of samples were positive for A. butzleri with the majority (29 of 32 isolates) originating from the evisceration machine. No Arcobacter spp. was isolated after cleaning. A. butzleri isolates confirmed by PCR were typed by multilocus sequence typing (MLST) resulting in 10 new sequence types (STs). Two sequence types were isolated on both processing days. Minimum inhibitory concentration (MIC) to sodium hypochlorite was determined to 0.5% hypochlorite biocide (500 ppm chlorine) for most isolates, which allows growth of A. butzleri within the working concentration of the biocide (0.2 - 0.5%). ConclusionsA. butzleri was readily isolated from a Danish broiler slaughterhouse, primarily in the evisceration machine. Typing by MLST showed high strain variability but the recurrence of two STs indicate that some persistence or cross-contamination takes place. Importantly, the isolates tolerated sodium hypochlorite, a biocide commonly employed in slaughterhouse sanitizing, at levels close to the disinfection concentration, and thus, A. butzleri may survive the disinfection process although this was not observed in our study.

    AB - BackgroundArcobacter spp. have in recent years received increasing interest as potential emerging enteropathogens and zoonotic agents. They are associated with various animals including poultry and can be isolated from meat products. The possibilities of persistence and cross-contamination in slaughterhouses during meat processing are not well established. We have evaluated the occurrence and persistence of Arcobacter spp. in a Danish slaughterhouse and determined the sensitivity of isolates to sodium hypochlorite, a commonly used biocide. ResultsArcobacter contamination was examined in a broiler slaughterhouse by selective enrichment of 235 swabs from the processing line during two production days and after sanitizing in between. In total 13.6% of samples were positive for A. butzleri with the majority (29 of 32 isolates) originating from the evisceration machine. No Arcobacter spp. was isolated after cleaning. A. butzleri isolates confirmed by PCR were typed by multilocus sequence typing (MLST) resulting in 10 new sequence types (STs). Two sequence types were isolated on both processing days. Minimum inhibitory concentration (MIC) to sodium hypochlorite was determined to 0.5% hypochlorite biocide (500 ppm chlorine) for most isolates, which allows growth of A. butzleri within the working concentration of the biocide (0.2 - 0.5%). ConclusionsA. butzleri was readily isolated from a Danish broiler slaughterhouse, primarily in the evisceration machine. Typing by MLST showed high strain variability but the recurrence of two STs indicate that some persistence or cross-contamination takes place. Importantly, the isolates tolerated sodium hypochlorite, a biocide commonly employed in slaughterhouse sanitizing, at levels close to the disinfection concentration, and thus, A. butzleri may survive the disinfection process although this was not observed in our study.

    KW - Arcobacter butzleri

    KW - MLST

    KW - Chicken slaughterhouse

    KW - MIC

    KW - Sodium hypochlorite

    KW - Arcobacter butzleri

    KW - MLST

    KW - Chicken slaughterhouse

    KW - MIC

    KW - Sodium hypochlorite

    U2 - 10.1186/1756-0500-6-322

    DO - 10.1186/1756-0500-6-322

    M3 - Journal article

    VL - 6

    JO - BMC Research Notes

    JF - BMC Research Notes

    SN - 1756-0500

    IS - 322

    ER -