Multi-walled carbon nanotube physicochemical properties predict pulmonary inflammation and genotoxicity

Sarah S. Poulsen, Petra Jackson, Kirsten Kling, Kristina B. Knudsen, Vidar Skaug, Zdenka O. Kyjovska, Birthe L. Thomsen, Per Axel Clausen, Rambabu Atluri, Trine Berthing, Stefan Bengtson, Henrik Wolff, Keld A. Jensen, Håkan Wallin, Ulla Vogel

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Lung deposition of multi-walled carbon nanotubes (MWCNT) induces pulmonary toxicity. Commercial MWCNT vary greatly in physicochemical properties and consequently in biological effects. To identify determinants of MWCNT-induced toxicity, we analyzed the effects of pulmonary exposure to 10 commercial MWCNT (supplied in three groups of different dimensions, with one pristine and two/three surface modified in each group). We characterized morphology, chemical composition, surface area and functionalization levels. MWCNT were deposited in lungs of female C57BL/6J mice by intratracheal instillation of 0, 6, 18 or 54 μg/mouse. Pulmonary inflammation (neutrophil influx in bronchoalveolar lavage (BAL)) and genotoxicity were determined on day 1, 28 or 92. Histopathology of the lungs was performed on day 28 and 92. All MWCNT induced similar histological changes. Lymphocytic aggregates were detected for all MWCNT on day 28 and 92. Using adjusted, multiple regression analyses, inflammation and genotoxicity were related to dose, time and physicochemical properties. The specific surface area (BET) was identified as a positive predictor of pulmonary inflammation on all post-exposure days. In addition, length significantly predicted pulmonary inflammation, whereas surface oxidation (–OH and –COOH) was predictor of lowered inflammation on day 28. BET surface area, and therefore diameter, significantly predicted genotoxicity in BAL fluid cells and lung tissue such that lower BET surface area or correspondingly larger diameter was associated with increased genotoxicity. This study provides information on possible toxicity-driving physicochemical properties of MWCNT. The results may contribute to safe-by-design manufacturing of MWCNT, thereby minimizing adverse effects.
OriginalsprogEngelsk
TidsskriftNanotoxicology
Vol/bind10
Udgave nummer9
Sider (fra-til)1263-1275
Antal sider13
ISSN1743-5390
DOI
StatusUdgivet - 2016
Udgivet eksterntJa

Citer dette

Poulsen, S. S., Jackson, P., Kling, K., Knudsen, K. B., Skaug, V., Kyjovska, Z. O., ... Vogel, U. (2016). Multi-walled carbon nanotube physicochemical properties predict pulmonary inflammation and genotoxicity. Nanotoxicology, 10(9), 1263-1275. https://doi.org/10.1080/17435390.2016.1202351
Poulsen, Sarah S. ; Jackson, Petra ; Kling, Kirsten ; Knudsen, Kristina B. ; Skaug, Vidar ; Kyjovska, Zdenka O. ; Thomsen, Birthe L. ; Clausen, Per Axel ; Atluri, Rambabu ; Berthing, Trine ; Bengtson, Stefan ; Wolff, Henrik ; Jensen, Keld A. ; Wallin, Håkan ; Vogel, Ulla. / Multi-walled carbon nanotube physicochemical properties predict pulmonary inflammation and genotoxicity. I: Nanotoxicology. 2016 ; Bind 10, Nr. 9. s. 1263-1275.
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title = "Multi-walled carbon nanotube physicochemical properties predict pulmonary inflammation and genotoxicity",
abstract = "Lung deposition of multi-walled carbon nanotubes (MWCNT) induces pulmonary toxicity. Commercial MWCNT vary greatly in physicochemical properties and consequently in biological effects. To identify determinants of MWCNT-induced toxicity, we analyzed the effects of pulmonary exposure to 10 commercial MWCNT (supplied in three groups of different dimensions, with one pristine and two/three surface modified in each group). We characterized morphology, chemical composition, surface area and functionalization levels. MWCNT were deposited in lungs of female C57BL/6J mice by intratracheal instillation of 0, 6, 18 or 54 μg/mouse. Pulmonary inflammation (neutrophil influx in bronchoalveolar lavage (BAL)) and genotoxicity were determined on day 1, 28 or 92. Histopathology of the lungs was performed on day 28 and 92. All MWCNT induced similar histological changes. Lymphocytic aggregates were detected for all MWCNT on day 28 and 92. Using adjusted, multiple regression analyses, inflammation and genotoxicity were related to dose, time and physicochemical properties. The specific surface area (BET) was identified as a positive predictor of pulmonary inflammation on all post-exposure days. In addition, length significantly predicted pulmonary inflammation, whereas surface oxidation (–OH and –COOH) was predictor of lowered inflammation on day 28. BET surface area, and therefore diameter, significantly predicted genotoxicity in BAL fluid cells and lung tissue such that lower BET surface area or correspondingly larger diameter was associated with increased genotoxicity. This study provides information on possible toxicity-driving physicochemical properties of MWCNT. The results may contribute to safe-by-design manufacturing of MWCNT, thereby minimizing adverse effects.",
author = "Poulsen, {Sarah S.} and Petra Jackson and Kirsten Kling and Knudsen, {Kristina B.} and Vidar Skaug and Kyjovska, {Zdenka O.} and Thomsen, {Birthe L.} and Clausen, {Per Axel} and Rambabu Atluri and Trine Berthing and Stefan Bengtson and Henrik Wolff and Jensen, {Keld A.} and H{\aa}kan Wallin and Ulla Vogel",
year = "2016",
doi = "10.1080/17435390.2016.1202351",
language = "English",
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Poulsen, SS, Jackson, P, Kling, K, Knudsen, KB, Skaug, V, Kyjovska, ZO, Thomsen, BL, Clausen, PA, Atluri, R, Berthing, T, Bengtson, S, Wolff, H, Jensen, KA, Wallin, H & Vogel, U 2016, 'Multi-walled carbon nanotube physicochemical properties predict pulmonary inflammation and genotoxicity' Nanotoxicology, bind 10, nr. 9, s. 1263-1275. https://doi.org/10.1080/17435390.2016.1202351

Multi-walled carbon nanotube physicochemical properties predict pulmonary inflammation and genotoxicity. / Poulsen, Sarah S.; Jackson, Petra; Kling, Kirsten; Knudsen, Kristina B.; Skaug, Vidar; Kyjovska, Zdenka O.; Thomsen, Birthe L.; Clausen, Per Axel; Atluri, Rambabu; Berthing, Trine; Bengtson, Stefan; Wolff, Henrik; Jensen, Keld A.; Wallin, Håkan; Vogel, Ulla.

I: Nanotoxicology, Bind 10, Nr. 9, 2016, s. 1263-1275.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Multi-walled carbon nanotube physicochemical properties predict pulmonary inflammation and genotoxicity

AU - Poulsen, Sarah S.

AU - Jackson, Petra

AU - Kling, Kirsten

AU - Knudsen, Kristina B.

AU - Skaug, Vidar

AU - Kyjovska, Zdenka O.

AU - Thomsen, Birthe L.

AU - Clausen, Per Axel

AU - Atluri, Rambabu

AU - Berthing, Trine

AU - Bengtson, Stefan

AU - Wolff, Henrik

AU - Jensen, Keld A.

AU - Wallin, Håkan

AU - Vogel, Ulla

PY - 2016

Y1 - 2016

N2 - Lung deposition of multi-walled carbon nanotubes (MWCNT) induces pulmonary toxicity. Commercial MWCNT vary greatly in physicochemical properties and consequently in biological effects. To identify determinants of MWCNT-induced toxicity, we analyzed the effects of pulmonary exposure to 10 commercial MWCNT (supplied in three groups of different dimensions, with one pristine and two/three surface modified in each group). We characterized morphology, chemical composition, surface area and functionalization levels. MWCNT were deposited in lungs of female C57BL/6J mice by intratracheal instillation of 0, 6, 18 or 54 μg/mouse. Pulmonary inflammation (neutrophil influx in bronchoalveolar lavage (BAL)) and genotoxicity were determined on day 1, 28 or 92. Histopathology of the lungs was performed on day 28 and 92. All MWCNT induced similar histological changes. Lymphocytic aggregates were detected for all MWCNT on day 28 and 92. Using adjusted, multiple regression analyses, inflammation and genotoxicity were related to dose, time and physicochemical properties. The specific surface area (BET) was identified as a positive predictor of pulmonary inflammation on all post-exposure days. In addition, length significantly predicted pulmonary inflammation, whereas surface oxidation (–OH and –COOH) was predictor of lowered inflammation on day 28. BET surface area, and therefore diameter, significantly predicted genotoxicity in BAL fluid cells and lung tissue such that lower BET surface area or correspondingly larger diameter was associated with increased genotoxicity. This study provides information on possible toxicity-driving physicochemical properties of MWCNT. The results may contribute to safe-by-design manufacturing of MWCNT, thereby minimizing adverse effects.

AB - Lung deposition of multi-walled carbon nanotubes (MWCNT) induces pulmonary toxicity. Commercial MWCNT vary greatly in physicochemical properties and consequently in biological effects. To identify determinants of MWCNT-induced toxicity, we analyzed the effects of pulmonary exposure to 10 commercial MWCNT (supplied in three groups of different dimensions, with one pristine and two/three surface modified in each group). We characterized morphology, chemical composition, surface area and functionalization levels. MWCNT were deposited in lungs of female C57BL/6J mice by intratracheal instillation of 0, 6, 18 or 54 μg/mouse. Pulmonary inflammation (neutrophil influx in bronchoalveolar lavage (BAL)) and genotoxicity were determined on day 1, 28 or 92. Histopathology of the lungs was performed on day 28 and 92. All MWCNT induced similar histological changes. Lymphocytic aggregates were detected for all MWCNT on day 28 and 92. Using adjusted, multiple regression analyses, inflammation and genotoxicity were related to dose, time and physicochemical properties. The specific surface area (BET) was identified as a positive predictor of pulmonary inflammation on all post-exposure days. In addition, length significantly predicted pulmonary inflammation, whereas surface oxidation (–OH and –COOH) was predictor of lowered inflammation on day 28. BET surface area, and therefore diameter, significantly predicted genotoxicity in BAL fluid cells and lung tissue such that lower BET surface area or correspondingly larger diameter was associated with increased genotoxicity. This study provides information on possible toxicity-driving physicochemical properties of MWCNT. The results may contribute to safe-by-design manufacturing of MWCNT, thereby minimizing adverse effects.

U2 - 10.1080/17435390.2016.1202351

DO - 10.1080/17435390.2016.1202351

M3 - Journal article

VL - 10

SP - 1263

EP - 1275

JO - Nanotoxicology

JF - Nanotoxicology

SN - 1743-5390

IS - 9

ER -