Model Membranes and Antimicrobial Activities of pH-Sensitive Copolymers

Greice K. Saraiva; Valdomiro V. de Souza; Luciana C. de Oliveira; Alyne Procópio; Maisa T. Martins; Isabel N. Aidar; Ronaldo C.F. Yi; Caroline D. Lacerda; Gustavo P.B. Carretero; Rafael B. de Lira; Karin A. Riske; Roberto K. Salinas; Hernan Chaimovich; Fábio H. Florenzano; Iolanda M. Cuccovia

doi:10.21577/0103-5053.20230079

Model Membranes and Antimicrobial Activities of pH-Sensitive Copolymers

Greice K. Saraiva, Valdomiro V. de Souza, Luciana C. de Oliveira, Alyne Procópio, Maisa T. Martins, Isabel N. Aidar, Ronaldo C.F. Yi, Caroline D. Lacerda, Gustavo P.B. Carretero, Rafael B. de Lira, Karin A. Riske, Roberto K. Salinas, Hernan Chaimovich, Fábio H. Florenzano, Iolanda M. Cuccovia^*

^*Corresponding author

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Polymers are options as antimicrobials for skin protection, antifouling surfaces, and fabrics. Here we analyzed the interaction of polymers based on poly(methacrylate) (PMMA) and poly((dimethylamino ethyl) methacrylate) (PDMAEMA) with model membranes and bacteria. We used the homopolymers PMMA, PDMAEMA, and the diblock copolymer(s) prepared with different PMMAm:PDMAEMAn ratios (m/n). The interactions of PDMAEMA and PMMAm-b-PDMAEMAn with large unilamellar vesicles (LUVs) prepared with phosphatidylcholine and phosphatidylglycerol at different pHs, were analyzed by nuclear magnetic resonance (NMR), dynamic light scattering, and zeta potential. These polymers promoted LUVs leakage of a fluorescent probe (5,6-carboxyfluorescein) localized exclusively in the internal aqueous compartment. Interestingly, all copolymers exhibit a bell-shaped pH dependence for the polymer-induced LUVs leakage. The interaction of the positively charged polymers and the pH effect was also demonstrated using giant unilamellar vesicles. These copolymers inhibited bacterial growth in the micromolar range and can be used to prevent bacterial growth on surfaces.

Originalsprog	Engelsk
Tidsskrift	Journal of the Brazilian Chemical Society
Vol/bind	34
Udgave nummer	12
Sider (fra-til)	1863-1876
Antal sider	14
ISSN	0103-5053
DOI	https://doi.org/10.21577/0103-5053.20230079
Status	Udgivet - 2023
Udgivet eksternt	Ja

Bibliografisk note

Funding Information:
IMC was responsible for conceptualization; GKS for lead investigation; VVS, AP, MTM, INA, RCFY, CDL, GPBC, RBL, LCO, KAR, RKS for support investigation; LCO, KAR, RKS, HC, FHF, IMC for formal analysis (equal); IMC, FHF for funding acquisition; LCO, RKS, HC, FHF, IMC for writing original draft and writing-review and editing.

Funding Information:
The authors thank Analytical Instrumentation Center of the University of São Paulo for NMR instrumentation. FHF thanks the National Council for Scientific and Technological Development, CNPq (grant No. 457733/2014-4), IMC, HC, and FHF thank FAPESP (Proc. 2013/08166-5). IMC thanks CNPq (grant No. 465259/2014-6). IMC and HC thank the Coordination for the Improvement of Higher Education Personnel (CAPES), the National Institute of Science and Technology Complex Fluids (INCT-FCx), and NAP-FCx (Núcleo de Apoio à Pesquisa de Fluidos Complexos da Universidade de São Paulo). G.P.B. Carretero thanks CAPES: INCT -Institutos Nacionais de Ciência e Tecnologia (Proc. 88887.137085/2017-00) and FAPESP (2018/15230-5). GKVS thanks CAPES (proc. no. 23038.004630/2014-35, Projeto Biocomputacional) and CNPq (proc. 457733/2014-4). VVS acknowledges CNPq for his fellowship. We thank Peter Park for his helpful suggestions.

Emneord

antimicrobial polymers
block copolymers
copolymer-induced vesicle leakage
membrane binding polymers
model membranes
pH-sensitive polymers

Link til dokument

10.21577/0103-5053.20230079

Citer dette

Saraiva, G. K., de Souza, V. V., de Oliveira, L. C., Procópio, A., Martins, M. T., Aidar, I. N., Yi, R. C. F., Lacerda, C. D., Carretero, G. P. B., de Lira, R. B., Riske, K. A., Salinas, R. K., Chaimovich, H., Florenzano, F. H., & Cuccovia, I. M. (2023). Model Membranes and Antimicrobial Activities of pH-Sensitive Copolymers. Journal of the Brazilian Chemical Society, 34(12), 1863-1876. https://doi.org/10.21577/0103-5053.20230079

@article{e039a937754e4ac3bb94167dea94c140,

title = "Model Membranes and Antimicrobial Activities of pH-Sensitive Copolymers",

abstract = "Polymers are options as antimicrobials for skin protection, antifouling surfaces, and fabrics. Here we analyzed the interaction of polymers based on poly(methacrylate) (PMMA) and poly((dimethylamino ethyl) methacrylate) (PDMAEMA) with model membranes and bacteria. We used the homopolymers PMMA, PDMAEMA, and the diblock copolymer(s) prepared with different PMMAm:PDMAEMAn ratios (m/n). The interactions of PDMAEMA and PMMAm-b-PDMAEMAn with large unilamellar vesicles (LUVs) prepared with phosphatidylcholine and phosphatidylglycerol at different pHs, were analyzed by nuclear magnetic resonance (NMR), dynamic light scattering, and zeta potential. These polymers promoted LUVs leakage of a fluorescent probe (5,6-carboxyfluorescein) localized exclusively in the internal aqueous compartment. Interestingly, all copolymers exhibit a bell-shaped pH dependence for the polymer-induced LUVs leakage. The interaction of the positively charged polymers and the pH effect was also demonstrated using giant unilamellar vesicles. These copolymers inhibited bacterial growth in the micromolar range and can be used to prevent bacterial growth on surfaces.",

keywords = "antimicrobial polymers, block copolymers, copolymer-induced vesicle leakage, membrane binding polymers, model membranes, pH-sensitive polymers, antimicrobial polymers, block copolymers, copolymer-induced vesicle leakage, membrane binding polymers, model membranes, pH-sensitive polymers",

author = "Saraiva, {Greice K.} and {de Souza}, {Valdomiro V.} and {de Oliveira}, {Luciana C.} and Alyne Proc{\'o}pio and Martins, {Maisa T.} and Aidar, {Isabel N.} and Yi, {Ronaldo C.F.} and Lacerda, {Caroline D.} and Carretero, {Gustavo P.B.} and {de Lira}, {Rafael B.} and Riske, {Karin A.} and Salinas, {Roberto K.} and Hernan Chaimovich and Florenzano, {F{\'a}bio H.} and Cuccovia, {Iolanda M.}",

note = "Funding Information: The authors thank Analytical Instrumentation Center of the University of S{\~a}o Paulo for NMR instrumentation. FHF thanks the National Council for Scientific and Technological Development, CNPq (grant No. 457733/2014-4), IMC, HC, and FHF thank FAPESP (Proc. 2013/08166-5). IMC thanks CNPq (grant No. 465259/2014-6). IMC and HC thank the Coordination for the Improvement of Higher Education Personnel (CAPES), the National Institute of Science and Technology Complex Fluids (INCT-FCx), and NAP-FCx (N{\'u}cleo de Apoio {\`a} Pesquisa de Fluidos Complexos da Universidade de S{\~a}o Paulo). G.P.B. Carretero thanks CAPES: INCT -Institutos Nacionais de Ci{\^e}ncia e Tecnologia (Proc. 88887.137085/2017-00) and FAPESP (2018/15230-5). GKVS thanks CAPES (proc. no. 23038.004630/2014-35, Projeto Biocomputacional) and CNPq (proc. 457733/2014-4).",

year = "2023",

doi = "10.21577/0103-5053.20230079",

language = "English",

volume = "34",

pages = "1863--1876",

journal = "Journal of the Brazilian Chemical Society",

issn = "0103-5053",

number = "12",

}

Saraiva, GK, de Souza, VV, de Oliveira, LC, Procópio, A, Martins, MT, Aidar, IN, Yi, RCF, Lacerda, CD, Carretero, GPB, de Lira, RB, Riske, KA, Salinas, RK, Chaimovich, H, Florenzano, FH & Cuccovia, IM 2023, 'Model Membranes and Antimicrobial Activities of pH-Sensitive Copolymers', Journal of the Brazilian Chemical Society, bind 34, nr. 12, s. 1863-1876. https://doi.org/10.21577/0103-5053.20230079

TY - JOUR

T1 - Model Membranes and Antimicrobial Activities of pH-Sensitive Copolymers

AU - Saraiva, Greice K.

AU - de Souza, Valdomiro V.

AU - de Oliveira, Luciana C.

AU - Procópio, Alyne

AU - Martins, Maisa T.

AU - Aidar, Isabel N.

AU - Yi, Ronaldo C.F.

AU - Lacerda, Caroline D.

AU - Carretero, Gustavo P.B.

AU - de Lira, Rafael B.

AU - Riske, Karin A.

AU - Salinas, Roberto K.

AU - Chaimovich, Hernan

AU - Florenzano, Fábio H.

AU - Cuccovia, Iolanda M.

N1 - Funding Information: The authors thank Analytical Instrumentation Center of the University of São Paulo for NMR instrumentation. FHF thanks the National Council for Scientific and Technological Development, CNPq (grant No. 457733/2014-4), IMC, HC, and FHF thank FAPESP (Proc. 2013/08166-5). IMC thanks CNPq (grant No. 465259/2014-6). IMC and HC thank the Coordination for the Improvement of Higher Education Personnel (CAPES), the National Institute of Science and Technology Complex Fluids (INCT-FCx), and NAP-FCx (Núcleo de Apoio à Pesquisa de Fluidos Complexos da Universidade de São Paulo). G.P.B. Carretero thanks CAPES: INCT -Institutos Nacionais de Ciência e Tecnologia (Proc. 88887.137085/2017-00) and FAPESP (2018/15230-5). GKVS thanks CAPES (proc. no. 23038.004630/2014-35, Projeto Biocomputacional) and CNPq (proc. 457733/2014-4).

PY - 2023

Y1 - 2023

N2 - Polymers are options as antimicrobials for skin protection, antifouling surfaces, and fabrics. Here we analyzed the interaction of polymers based on poly(methacrylate) (PMMA) and poly((dimethylamino ethyl) methacrylate) (PDMAEMA) with model membranes and bacteria. We used the homopolymers PMMA, PDMAEMA, and the diblock copolymer(s) prepared with different PMMAm:PDMAEMAn ratios (m/n). The interactions of PDMAEMA and PMMAm-b-PDMAEMAn with large unilamellar vesicles (LUVs) prepared with phosphatidylcholine and phosphatidylglycerol at different pHs, were analyzed by nuclear magnetic resonance (NMR), dynamic light scattering, and zeta potential. These polymers promoted LUVs leakage of a fluorescent probe (5,6-carboxyfluorescein) localized exclusively in the internal aqueous compartment. Interestingly, all copolymers exhibit a bell-shaped pH dependence for the polymer-induced LUVs leakage. The interaction of the positively charged polymers and the pH effect was also demonstrated using giant unilamellar vesicles. These copolymers inhibited bacterial growth in the micromolar range and can be used to prevent bacterial growth on surfaces.

AB - Polymers are options as antimicrobials for skin protection, antifouling surfaces, and fabrics. Here we analyzed the interaction of polymers based on poly(methacrylate) (PMMA) and poly((dimethylamino ethyl) methacrylate) (PDMAEMA) with model membranes and bacteria. We used the homopolymers PMMA, PDMAEMA, and the diblock copolymer(s) prepared with different PMMAm:PDMAEMAn ratios (m/n). The interactions of PDMAEMA and PMMAm-b-PDMAEMAn with large unilamellar vesicles (LUVs) prepared with phosphatidylcholine and phosphatidylglycerol at different pHs, were analyzed by nuclear magnetic resonance (NMR), dynamic light scattering, and zeta potential. These polymers promoted LUVs leakage of a fluorescent probe (5,6-carboxyfluorescein) localized exclusively in the internal aqueous compartment. Interestingly, all copolymers exhibit a bell-shaped pH dependence for the polymer-induced LUVs leakage. The interaction of the positively charged polymers and the pH effect was also demonstrated using giant unilamellar vesicles. These copolymers inhibited bacterial growth in the micromolar range and can be used to prevent bacterial growth on surfaces.

KW - antimicrobial polymers

KW - block copolymers

KW - copolymer-induced vesicle leakage

KW - membrane binding polymers

KW - model membranes

KW - pH-sensitive polymers

KW - antimicrobial polymers

KW - block copolymers

KW - copolymer-induced vesicle leakage

KW - membrane binding polymers

KW - model membranes

KW - pH-sensitive polymers

U2 - 10.21577/0103-5053.20230079

DO - 10.21577/0103-5053.20230079

M3 - Journal article

AN - SCOPUS:85176218952

SN - 0103-5053

VL - 34

SP - 1863

EP - 1876

JO - Journal of the Brazilian Chemical Society

JF - Journal of the Brazilian Chemical Society

IS - 12

ER -