Metabolomics of early myocardial ischemia

Rasmus Bo Hasselbalch, Jonas Henrik Kristensen, Nina Strandkjær, Nicoline Jørgensen, Henning Bundgaard, Anders Malmendal, Kasper Karmark Iversen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstract

INTRODUCTION: Diagnosing myocardial infarction is difficult during the initial phase. As, acute myocardial ischemia is associated with changes in metabolic pathways, metabolomics may provide ways of identifying early stages of ischemia. We investigated the changes in metabolites after induced ischemia in humans using nuclear magnetic resonance spectroscopy (NMR). METHODS: We included patients undergoing elective coronary angiography showing normal coronary arteries. These were randomized into 4 groups and underwent coronary artery occlusion for 0, 30, 60 or 90 s. Blood was collected over the next 3 h and analyzed using NMR. We used 2-way ANOVA of time from baseline- and treatment group to find metabolites that changed significantly following the intervention and principal component analysis (PCA) to investigate changes between the 90 s ischemia- and control groups at 15 and 60 min after intervention. RESULTS: We included 34 patients. The most pronounced changes were observed in the lipid metabolism where 38 of 112 lipoprotein parameters (34%) showed a significant difference between the patients exposed to ischemia and the control group. There was a decrease in total plasma triglycerides over the first hour followed by a normalization. The principal component analysis showed a effects of the treatment after just 15 min. These effects were dominated by changes in high-density lipoprotein. An increase in lactic acid levels was detected surprisingly late, 1-2 h after the ischemia. CONCLUSION: We investigated the earliest changes in metabolites of patients undergoing brief myocardial ischemia and found that ischemia led to changes throughout the lipid metabolism as early as 15 min post-intervention.

OriginalsprogEngelsk
Artikelnummer33
TidsskriftMetabolomics : Official journal of the Metabolomic Society
Vol/bind19
Udgave nummer4
Sider (fra-til)33
Antal sider1
ISSN1573-3890
DOI
StatusUdgivet - 1 apr. 2023

Emneord

  • Coronary angiography
  • Metabolomics
  • Myocardial infarction
  • Myocardial ischemia
  • NMR

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