Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogaster

Kamilla Sofie Pedersen, Torsten Nygaard Kristensen, Volker Loeschcke, Bent O Petersen, Jens Ø Duus, Niels Chr Nielsen, Anders Malmendal

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

While the population genetics of inbreeding is fairly well understood, the effects of inbreeding on the physiological and biochemical levels are not. Here we have investigated the effects of inbreeding on the Drosophila melanogaster metabolome. Metabolite fingerprints in males from five outbred and five inbred lines were studied by nuclear magnetic resonance spectroscopy after exposure to benign temperature, heat stress, or cold stress. In both the absence and the presence of temperature stress, metabolite levels were significantly different among inbred and outbred lines. The major effect of inbreeding was increased levels of maltose and decreased levels of 3-hydroxykynurenine and a galactoside [1-O-(4-O-(2-aminoethyl phosphate)-beta-d-galactopyranosyl)-x-glycerol] synthesized exclusively in the paragonial glands of Drosophila species, including D. melanogaster. The metabolomic effect of inbreeding at the benign temperature was related to gene expression data from the same inbred and outbred lines. Both gene expression and metabolite data indicate that fundamental metabolic processes are changed or modified by inbreeding. Apart from affecting mean metabolite levels, inbreeding led to an increased between-line variation in metabolite profiles compared to outbred lines. In contrast to previous observations revealing interactions between inbreeding and environmental stress on gene expression patterns and life-history traits, the effect of inbreeding on the metabolite profile was similar across the different temperature treatments.
OriginalsprogEngelsk
TidsskriftGenetics
Vol/bind180
Udgave nummer2
Sider (fra-til)1233-43
Antal sider11
ISSN0016-6731
DOI
StatusUdgivet - 1 okt. 2008
Udgivet eksterntJa

Citer dette

Pedersen, K. S., Kristensen, T. N., Loeschcke, V., Petersen, B. O., Duus, J. Ø., Nielsen, N. C., & Malmendal, A. (2008). Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogaster. Genetics, 180(2), 1233-43. https://doi.org/10.1534/genetics.108.089144
Pedersen, Kamilla Sofie ; Kristensen, Torsten Nygaard ; Loeschcke, Volker ; Petersen, Bent O ; Duus, Jens Ø ; Nielsen, Niels Chr ; Malmendal, Anders. / Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogaster. I: Genetics. 2008 ; Bind 180, Nr. 2. s. 1233-43.
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abstract = "While the population genetics of inbreeding is fairly well understood, the effects of inbreeding on the physiological and biochemical levels are not. Here we have investigated the effects of inbreeding on the Drosophila melanogaster metabolome. Metabolite fingerprints in males from five outbred and five inbred lines were studied by nuclear magnetic resonance spectroscopy after exposure to benign temperature, heat stress, or cold stress. In both the absence and the presence of temperature stress, metabolite levels were significantly different among inbred and outbred lines. The major effect of inbreeding was increased levels of maltose and decreased levels of 3-hydroxykynurenine and a galactoside [1-O-(4-O-(2-aminoethyl phosphate)-beta-d-galactopyranosyl)-x-glycerol] synthesized exclusively in the paragonial glands of Drosophila species, including D. melanogaster. The metabolomic effect of inbreeding at the benign temperature was related to gene expression data from the same inbred and outbred lines. Both gene expression and metabolite data indicate that fundamental metabolic processes are changed or modified by inbreeding. Apart from affecting mean metabolite levels, inbreeding led to an increased between-line variation in metabolite profiles compared to outbred lines. In contrast to previous observations revealing interactions between inbreeding and environmental stress on gene expression patterns and life-history traits, the effect of inbreeding on the metabolite profile was similar across the different temperature treatments.",
author = "Pedersen, {Kamilla Sofie} and Kristensen, {Torsten Nygaard} and Volker Loeschcke and Petersen, {Bent O} and Duus, {Jens {\O}} and Nielsen, {Niels Chr} and Anders Malmendal",
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Pedersen, KS, Kristensen, TN, Loeschcke, V, Petersen, BO, Duus, JØ, Nielsen, NC & Malmendal, A 2008, 'Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogaster', Genetics, bind 180, nr. 2, s. 1233-43. https://doi.org/10.1534/genetics.108.089144

Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogaster. / Pedersen, Kamilla Sofie; Kristensen, Torsten Nygaard; Loeschcke, Volker; Petersen, Bent O; Duus, Jens Ø; Nielsen, Niels Chr; Malmendal, Anders.

I: Genetics, Bind 180, Nr. 2, 01.10.2008, s. 1233-43.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogaster

AU - Pedersen, Kamilla Sofie

AU - Kristensen, Torsten Nygaard

AU - Loeschcke, Volker

AU - Petersen, Bent O

AU - Duus, Jens Ø

AU - Nielsen, Niels Chr

AU - Malmendal, Anders

PY - 2008/10/1

Y1 - 2008/10/1

N2 - While the population genetics of inbreeding is fairly well understood, the effects of inbreeding on the physiological and biochemical levels are not. Here we have investigated the effects of inbreeding on the Drosophila melanogaster metabolome. Metabolite fingerprints in males from five outbred and five inbred lines were studied by nuclear magnetic resonance spectroscopy after exposure to benign temperature, heat stress, or cold stress. In both the absence and the presence of temperature stress, metabolite levels were significantly different among inbred and outbred lines. The major effect of inbreeding was increased levels of maltose and decreased levels of 3-hydroxykynurenine and a galactoside [1-O-(4-O-(2-aminoethyl phosphate)-beta-d-galactopyranosyl)-x-glycerol] synthesized exclusively in the paragonial glands of Drosophila species, including D. melanogaster. The metabolomic effect of inbreeding at the benign temperature was related to gene expression data from the same inbred and outbred lines. Both gene expression and metabolite data indicate that fundamental metabolic processes are changed or modified by inbreeding. Apart from affecting mean metabolite levels, inbreeding led to an increased between-line variation in metabolite profiles compared to outbred lines. In contrast to previous observations revealing interactions between inbreeding and environmental stress on gene expression patterns and life-history traits, the effect of inbreeding on the metabolite profile was similar across the different temperature treatments.

AB - While the population genetics of inbreeding is fairly well understood, the effects of inbreeding on the physiological and biochemical levels are not. Here we have investigated the effects of inbreeding on the Drosophila melanogaster metabolome. Metabolite fingerprints in males from five outbred and five inbred lines were studied by nuclear magnetic resonance spectroscopy after exposure to benign temperature, heat stress, or cold stress. In both the absence and the presence of temperature stress, metabolite levels were significantly different among inbred and outbred lines. The major effect of inbreeding was increased levels of maltose and decreased levels of 3-hydroxykynurenine and a galactoside [1-O-(4-O-(2-aminoethyl phosphate)-beta-d-galactopyranosyl)-x-glycerol] synthesized exclusively in the paragonial glands of Drosophila species, including D. melanogaster. The metabolomic effect of inbreeding at the benign temperature was related to gene expression data from the same inbred and outbred lines. Both gene expression and metabolite data indicate that fundamental metabolic processes are changed or modified by inbreeding. Apart from affecting mean metabolite levels, inbreeding led to an increased between-line variation in metabolite profiles compared to outbred lines. In contrast to previous observations revealing interactions between inbreeding and environmental stress on gene expression patterns and life-history traits, the effect of inbreeding on the metabolite profile was similar across the different temperature treatments.

U2 - 10.1534/genetics.108.089144

DO - 10.1534/genetics.108.089144

M3 - Journal article

VL - 180

SP - 1233

EP - 1243

JO - Genetics (Print)

JF - Genetics (Print)

SN - 0016-6731

IS - 2

ER -

Pedersen KS, Kristensen TN, Loeschcke V, Petersen BO, Duus JØ, Nielsen NC et al. Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogaster. Genetics. 2008 okt 1;180(2):1233-43. https://doi.org/10.1534/genetics.108.089144