TY - JOUR
T1 - Metabolic characterization of rumen epithelial tissue from dairy calves fed different starter diets using 1H NMR spectroscopy
AU - Bertram, H.C.
AU - Kristensen, Niels Bastian
AU - Vestergaard, M.
AU - Jensen, S.K.
AU - Sehested, J.
AU - Nielsen, N.C.
AU - Malmendal, A.
PY - 2009
Y1 - 2009
N2 - The metabolic profile of calf rumen epithelial tissue was for the first time characterized by 1H NMR spectroscopy of chloroform/methanol extracts. The metabolite profiles comprised a number of amino acids, creatine, taurine, short-chain fatty acids and triglycerides. The effects of two dietary interventions; i) four levels of milk allowance with a concomitant different uptake of starter concentrate, and ii) two diets with varying content of starch and fibre in the concentrate, were elucidated. Partial least square regression analysis revealed that the intensity of NMR signals assigned to leucine, isoleucine and valine (0.90 \ppm), propionate (1.06 and 2.18 \ppm), lactate (1.32 \ppm), butyrate (1.56 \ppm), acetate (1.93 \ppm), glutamine/glutamate (2.18, 2.35 and 3.75 \ppm), creatine (3.04 and 3.94 \ppm) and glycine (3.55 \ppm) decreased with increasing milk allowance. In addition, the analysis revealed that the main difference between the two diets was the content of propionate in the epithelia tissue extracts. Previous morphological analyses of the same rumen epithelia were not able to detect any significant effects of either milk allowance or dietary starch content. Accordingly, the present study demonstrated that 1H NMR spectroscopy applied on extracts is a useful tool for metabolite profiling of epithelial tissue and for following the development of epithelia tissue in young calves, and that the technique may be more sensitive to dietary effects than morphological studies. textcopyright 2008 Elsevier B.V. All rights reserved.
AB - The metabolic profile of calf rumen epithelial tissue was for the first time characterized by 1H NMR spectroscopy of chloroform/methanol extracts. The metabolite profiles comprised a number of amino acids, creatine, taurine, short-chain fatty acids and triglycerides. The effects of two dietary interventions; i) four levels of milk allowance with a concomitant different uptake of starter concentrate, and ii) two diets with varying content of starch and fibre in the concentrate, were elucidated. Partial least square regression analysis revealed that the intensity of NMR signals assigned to leucine, isoleucine and valine (0.90 \ppm), propionate (1.06 and 2.18 \ppm), lactate (1.32 \ppm), butyrate (1.56 \ppm), acetate (1.93 \ppm), glutamine/glutamate (2.18, 2.35 and 3.75 \ppm), creatine (3.04 and 3.94 \ppm) and glycine (3.55 \ppm) decreased with increasing milk allowance. In addition, the analysis revealed that the main difference between the two diets was the content of propionate in the epithelia tissue extracts. Previous morphological analyses of the same rumen epithelia were not able to detect any significant effects of either milk allowance or dietary starch content. Accordingly, the present study demonstrated that 1H NMR spectroscopy applied on extracts is a useful tool for metabolite profiling of epithelial tissue and for following the development of epithelia tissue in young calves, and that the technique may be more sensitive to dietary effects than morphological studies. textcopyright 2008 Elsevier B.V. All rights reserved.
KW - Cattle
KW - Extracts
KW - Metabonomics
KW - Milk allowance
KW - NMR
KW - Short-chain fatty acids
KW - Starch
U2 - 10.1016/j.livsci.2008.05.001
DO - 10.1016/j.livsci.2008.05.001
M3 - Journal article
VL - 120
SP - 127
EP - 134
JO - Livestock Science
JF - Livestock Science
SN - 1871-1413
IS - 1-2
ER -