Metabolic characterization of rumen epithelial tissue from dairy calves fed different starter diets using 1H NMR spectroscopy

H.C. Bertram, N.B. Kristensen, M. Vestergaard, S.K. Jensen, J. Sehested, N.C. Nielsen, A. Malmendal

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The metabolic profile of calf rumen epithelial tissue was for the first time characterized by 1H NMR spectroscopy of chloroform/methanol extracts. The metabolite profiles comprised a number of amino acids, creatine, taurine, short-chain fatty acids and triglycerides. The effects of two dietary interventions; i) four levels of milk allowance with a concomitant different uptake of starter concentrate, and ii) two diets with varying content of starch and fibre in the concentrate, were elucidated. Partial least square regression analysis revealed that the intensity of NMR signals assigned to leucine, isoleucine and valine (0.90 \ppm), propionate (1.06 and 2.18 \ppm), lactate (1.32 \ppm), butyrate (1.56 \ppm), acetate (1.93 \ppm), glutamine/glutamate (2.18, 2.35 and 3.75 \ppm), creatine (3.04 and 3.94 \ppm) and glycine (3.55 \ppm) decreased with increasing milk allowance. In addition, the analysis revealed that the main difference between the two diets was the content of propionate in the epithelia tissue extracts. Previous morphological analyses of the same rumen epithelia were not able to detect any significant effects of either milk allowance or dietary starch content. Accordingly, the present study demonstrated that 1H NMR spectroscopy applied on extracts is a useful tool for metabolite profiling of epithelial tissue and for following the development of epithelia tissue in young calves, and that the technique may be more sensitive to dietary effects than morphological studies. textcopyright 2008 Elsevier B.V. All rights reserved.
OriginalsprogEngelsk
TidsskriftLivestock Science
Vol/bind120
Udgave nummer1-2
DOI
StatusUdgivet - 2009

Emneord

  • Cattle
  • Extracts
  • Metabonomics
  • Milk allowance
  • NMR
  • Short-chain fatty acids
  • Starch

Citer dette

@article{a2b53723cac74a21821691a3e56ca436,
title = "Metabolic characterization of rumen epithelial tissue from dairy calves fed different starter diets using 1H NMR spectroscopy",
abstract = "The metabolic profile of calf rumen epithelial tissue was for the first time characterized by 1H NMR spectroscopy of chloroform/methanol extracts. The metabolite profiles comprised a number of amino acids, creatine, taurine, short-chain fatty acids and triglycerides. The effects of two dietary interventions; i) four levels of milk allowance with a concomitant different uptake of starter concentrate, and ii) two diets with varying content of starch and fibre in the concentrate, were elucidated. Partial least square regression analysis revealed that the intensity of NMR signals assigned to leucine, isoleucine and valine (0.90 \ppm), propionate (1.06 and 2.18 \ppm), lactate (1.32 \ppm), butyrate (1.56 \ppm), acetate (1.93 \ppm), glutamine/glutamate (2.18, 2.35 and 3.75 \ppm), creatine (3.04 and 3.94 \ppm) and glycine (3.55 \ppm) decreased with increasing milk allowance. In addition, the analysis revealed that the main difference between the two diets was the content of propionate in the epithelia tissue extracts. Previous morphological analyses of the same rumen epithelia were not able to detect any significant effects of either milk allowance or dietary starch content. Accordingly, the present study demonstrated that 1H NMR spectroscopy applied on extracts is a useful tool for metabolite profiling of epithelial tissue and for following the development of epithelia tissue in young calves, and that the technique may be more sensitive to dietary effects than morphological studies. textcopyright 2008 Elsevier B.V. All rights reserved.",
keywords = "Cattle, Extracts, Metabonomics, Milk allowance, NMR, Short-chain fatty acids, Starch",
author = "H.C. Bertram and N.B. Kristensen and M. Vestergaard and S.K. Jensen and J. Sehested and N.C. Nielsen and A. Malmendal",
year = "2009",
doi = "10.1016/j.livsci.2008.05.001",
language = "English",
volume = "120",
journal = "Livestock Science",
number = "1-2",

}

Metabolic characterization of rumen epithelial tissue from dairy calves fed different starter diets using 1H NMR spectroscopy. / Bertram, H.C.; Kristensen, N.B.; Vestergaard, M.; Jensen, S.K.; Sehested, J.; Nielsen, N.C.; Malmendal, A.

I: Livestock Science, Bind 120, Nr. 1-2, 2009.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Metabolic characterization of rumen epithelial tissue from dairy calves fed different starter diets using 1H NMR spectroscopy

AU - Bertram, H.C.

AU - Kristensen, N.B.

AU - Vestergaard, M.

AU - Jensen, S.K.

AU - Sehested, J.

AU - Nielsen, N.C.

AU - Malmendal, A.

PY - 2009

Y1 - 2009

N2 - The metabolic profile of calf rumen epithelial tissue was for the first time characterized by 1H NMR spectroscopy of chloroform/methanol extracts. The metabolite profiles comprised a number of amino acids, creatine, taurine, short-chain fatty acids and triglycerides. The effects of two dietary interventions; i) four levels of milk allowance with a concomitant different uptake of starter concentrate, and ii) two diets with varying content of starch and fibre in the concentrate, were elucidated. Partial least square regression analysis revealed that the intensity of NMR signals assigned to leucine, isoleucine and valine (0.90 \ppm), propionate (1.06 and 2.18 \ppm), lactate (1.32 \ppm), butyrate (1.56 \ppm), acetate (1.93 \ppm), glutamine/glutamate (2.18, 2.35 and 3.75 \ppm), creatine (3.04 and 3.94 \ppm) and glycine (3.55 \ppm) decreased with increasing milk allowance. In addition, the analysis revealed that the main difference between the two diets was the content of propionate in the epithelia tissue extracts. Previous morphological analyses of the same rumen epithelia were not able to detect any significant effects of either milk allowance or dietary starch content. Accordingly, the present study demonstrated that 1H NMR spectroscopy applied on extracts is a useful tool for metabolite profiling of epithelial tissue and for following the development of epithelia tissue in young calves, and that the technique may be more sensitive to dietary effects than morphological studies. textcopyright 2008 Elsevier B.V. All rights reserved.

AB - The metabolic profile of calf rumen epithelial tissue was for the first time characterized by 1H NMR spectroscopy of chloroform/methanol extracts. The metabolite profiles comprised a number of amino acids, creatine, taurine, short-chain fatty acids and triglycerides. The effects of two dietary interventions; i) four levels of milk allowance with a concomitant different uptake of starter concentrate, and ii) two diets with varying content of starch and fibre in the concentrate, were elucidated. Partial least square regression analysis revealed that the intensity of NMR signals assigned to leucine, isoleucine and valine (0.90 \ppm), propionate (1.06 and 2.18 \ppm), lactate (1.32 \ppm), butyrate (1.56 \ppm), acetate (1.93 \ppm), glutamine/glutamate (2.18, 2.35 and 3.75 \ppm), creatine (3.04 and 3.94 \ppm) and glycine (3.55 \ppm) decreased with increasing milk allowance. In addition, the analysis revealed that the main difference between the two diets was the content of propionate in the epithelia tissue extracts. Previous morphological analyses of the same rumen epithelia were not able to detect any significant effects of either milk allowance or dietary starch content. Accordingly, the present study demonstrated that 1H NMR spectroscopy applied on extracts is a useful tool for metabolite profiling of epithelial tissue and for following the development of epithelia tissue in young calves, and that the technique may be more sensitive to dietary effects than morphological studies. textcopyright 2008 Elsevier B.V. All rights reserved.

KW - Cattle

KW - Extracts

KW - Metabonomics

KW - Milk allowance

KW - NMR

KW - Short-chain fatty acids

KW - Starch

U2 - 10.1016/j.livsci.2008.05.001

DO - 10.1016/j.livsci.2008.05.001

M3 - Journal article

VL - 120

JO - Livestock Science

JF - Livestock Science

IS - 1-2

ER -