Metabolic and functional characterization of effects of developmental temperature in Drosophila melanogaster

Mads F Schou, Torsten Nygaard Kristensen, Anders Pedersen, Goran B Karlsson, Volker Loeschcke, Anders Malmendal

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The ability of ectotherms to respond to changes in their thermal environment through plastic mechanisms is central to their adaptive capability. However, we still lack knowledge on physiological and functional responses by which ectotherms acclimate to temperatures during development, and in particular, how physiological stress at extreme temperatures may counteract beneficial acclimation responses at benign temperatures. We exposed Drosophila melanogaster to ten developmental temperatures covering their entire permissible temperature range. We obtained metabolic profiles and reaction norms for several functional traits: egg-to-adult viability, developmental time, and heat and cold tolerance. Females were more heat tolerant than males, whereas no sexual dimorphism was found in cold tolerance. A group of metabolites, mainly free amino acids, had linear reaction norms. Several energy carrying molecules, as well as some sugars, showed distinct inverted u-shaped norms of reaction across the thermal range, resulting in a positive correlation between metabolite intensities and egg-to-adult viability. At extreme temperatures, low levels of these metabolites were interpreted as a response characteristic of costs of homeostatic perturbations. Our results provide novel insights into a range of metabolites reported to be central for the acclimation response, and suggest several new candidate metabolites. Low and high temperatures result in different adaptive physiological responses, but they also have commonalities likely to be a result of the failure to compensate for the physiological stress. We suggest that the regulation of metabolites that are tightly connected to the performance curve is important for the ability of ectotherms to cope with variation in temperature.

OriginalsprogEngelsk
TidsskriftAmerican Journal of Physiology: Regulatory, Integrative and Comparative Physiology
Vol/bind312
Udgave nummer2
Sider (fra-til)R211-R222
ISSN0363-6119
DOI
StatusUdgivet - feb. 2017
Udgivet eksterntJa

Citer dette

Schou, Mads F ; Kristensen, Torsten Nygaard ; Pedersen, Anders ; Karlsson, Goran B ; Loeschcke, Volker ; Malmendal, Anders. / Metabolic and functional characterization of effects of developmental temperature in Drosophila melanogaster. I: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology. 2017 ; Bind 312, Nr. 2. s. R211-R222.
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title = "Metabolic and functional characterization of effects of developmental temperature in Drosophila melanogaster",
abstract = "The ability of ectotherms to respond to changes in their thermal environment through plastic mechanisms is central to their adaptive capability. However, we still lack knowledge on physiological and functional responses by which ectotherms acclimate to temperatures during development, and in particular, how physiological stress at extreme temperatures may counteract beneficial acclimation responses at benign temperatures. We exposed Drosophila melanogaster to ten developmental temperatures covering their entire permissible temperature range. We obtained metabolic profiles and reaction norms for several functional traits: egg-to-adult viability, developmental time, and heat and cold tolerance. Females were more heat tolerant than males, whereas no sexual dimorphism was found in cold tolerance. A group of metabolites, mainly free amino acids, had linear reaction norms. Several energy carrying molecules, as well as some sugars, showed distinct inverted u-shaped norms of reaction across the thermal range, resulting in a positive correlation between metabolite intensities and egg-to-adult viability. At extreme temperatures, low levels of these metabolites were interpreted as a response characteristic of costs of homeostatic perturbations. Our results provide novel insights into a range of metabolites reported to be central for the acclimation response, and suggest several new candidate metabolites. Low and high temperatures result in different adaptive physiological responses, but they also have commonalities likely to be a result of the failure to compensate for the physiological stress. We suggest that the regulation of metabolites that are tightly connected to the performance curve is important for the ability of ectotherms to cope with variation in temperature.",
author = "Schou, {Mads F} and Kristensen, {Torsten Nygaard} and Anders Pedersen and Karlsson, {Goran B} and Volker Loeschcke and Anders Malmendal",
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Metabolic and functional characterization of effects of developmental temperature in Drosophila melanogaster. / Schou, Mads F; Kristensen, Torsten Nygaard; Pedersen, Anders; Karlsson, Goran B; Loeschcke, Volker; Malmendal, Anders.

I: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, Bind 312, Nr. 2, 02.2017, s. R211-R222.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Metabolic and functional characterization of effects of developmental temperature in Drosophila melanogaster

AU - Schou, Mads F

AU - Kristensen, Torsten Nygaard

AU - Pedersen, Anders

AU - Karlsson, Goran B

AU - Loeschcke, Volker

AU - Malmendal, Anders

N1 - Copyright © 2016, American Journal of Physiology-Regulatory, Integrative and Comparative Physiology.

PY - 2017/2

Y1 - 2017/2

N2 - The ability of ectotherms to respond to changes in their thermal environment through plastic mechanisms is central to their adaptive capability. However, we still lack knowledge on physiological and functional responses by which ectotherms acclimate to temperatures during development, and in particular, how physiological stress at extreme temperatures may counteract beneficial acclimation responses at benign temperatures. We exposed Drosophila melanogaster to ten developmental temperatures covering their entire permissible temperature range. We obtained metabolic profiles and reaction norms for several functional traits: egg-to-adult viability, developmental time, and heat and cold tolerance. Females were more heat tolerant than males, whereas no sexual dimorphism was found in cold tolerance. A group of metabolites, mainly free amino acids, had linear reaction norms. Several energy carrying molecules, as well as some sugars, showed distinct inverted u-shaped norms of reaction across the thermal range, resulting in a positive correlation between metabolite intensities and egg-to-adult viability. At extreme temperatures, low levels of these metabolites were interpreted as a response characteristic of costs of homeostatic perturbations. Our results provide novel insights into a range of metabolites reported to be central for the acclimation response, and suggest several new candidate metabolites. Low and high temperatures result in different adaptive physiological responses, but they also have commonalities likely to be a result of the failure to compensate for the physiological stress. We suggest that the regulation of metabolites that are tightly connected to the performance curve is important for the ability of ectotherms to cope with variation in temperature.

AB - The ability of ectotherms to respond to changes in their thermal environment through plastic mechanisms is central to their adaptive capability. However, we still lack knowledge on physiological and functional responses by which ectotherms acclimate to temperatures during development, and in particular, how physiological stress at extreme temperatures may counteract beneficial acclimation responses at benign temperatures. We exposed Drosophila melanogaster to ten developmental temperatures covering their entire permissible temperature range. We obtained metabolic profiles and reaction norms for several functional traits: egg-to-adult viability, developmental time, and heat and cold tolerance. Females were more heat tolerant than males, whereas no sexual dimorphism was found in cold tolerance. A group of metabolites, mainly free amino acids, had linear reaction norms. Several energy carrying molecules, as well as some sugars, showed distinct inverted u-shaped norms of reaction across the thermal range, resulting in a positive correlation between metabolite intensities and egg-to-adult viability. At extreme temperatures, low levels of these metabolites were interpreted as a response characteristic of costs of homeostatic perturbations. Our results provide novel insights into a range of metabolites reported to be central for the acclimation response, and suggest several new candidate metabolites. Low and high temperatures result in different adaptive physiological responses, but they also have commonalities likely to be a result of the failure to compensate for the physiological stress. We suggest that the regulation of metabolites that are tightly connected to the performance curve is important for the ability of ectotherms to cope with variation in temperature.

U2 - 10.1152/ajpregu.00268.2016

DO - 10.1152/ajpregu.00268.2016

M3 - Journal article

VL - 312

SP - R211-R222

JO - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology

JF - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology

SN - 0363-6119

IS - 2

ER -