Maternal inhalation of surface-coated nanosized titanium dioxide (UV-Titan) in C57BL/6 mice: effects in prenatally exposed offspring on hepatic DNA damage and gene expression

Ole Andersen, Petra Jackson, Sabina Halappanavar, Karin Sørig Hougaard, Andrew Williams, Anne Mette Madsen, Jacob Stuart Lamson, Caroline Yauk, Erik Håkan Richard Wallin, Ulla Birgitte Vogel

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    Abstract

    We investigated effects of maternal pulmonary exposure to titanium dioxide (UV-Titan) on prenatally exposed offspring. Time-mated mice (C57BL/6BomTac) were inhalation exposed (1 h/day to 42 mg UV-Titan/m3 aerosolised powder or filtered air) during gestation days (GDs) 8–18. We evaluated DNA strand breaks using the comet assay in bronchoalveolar lavage (BAL) cells and livers of the time-mated mice (5 and 26–27 days after inhalation exposure), and in livers of the offspring (post-natal days (PND) 2 and 22). We also analysed hepatic gene expression in newborns using DNA microarrays. UV-Titan exposure did not induce DNA strand breaks in time-mated mice or their offspring. Transcriptional profiling of newborn livers revealed changes in the gene expression related to the retinoic acid signalling pathway in the females, while gene expression in male offspring was unaffected. Changes may be a secondary response to maternal inflammation although no direct link was evident through gene expression analysis.
    OriginalsprogEngelsk
    TidsskriftNanotoxicology
    Vol/bind7
    Udgave nummer1
    Sider (fra-til)85-96
    ISSN1743-5390
    DOI
    StatusUdgivet - feb. 2013

    Emneord

    • In utero exposure
    • lung inflammation
    • DNA strand breaks
    • retinoic acid pathway
    • acute-phase response

    Citer dette

    Andersen, O., Jackson, P., Halappanavar, S., Sørig Hougaard, K., Williams, A., Madsen, A. M., Stuart Lamson, J., Yauk, C., Wallin, E. H. R., & Vogel, U. B. (2013). Maternal inhalation of surface-coated nanosized titanium dioxide (UV-Titan) in C57BL/6 mice: effects in prenatally exposed offspring on hepatic DNA damage and gene expression. Nanotoxicology, 7(1), 85-96. https://doi.org/10.3109/17435390.2011.633715