Maternal inhalation of surface-coated nanosized titanium dioxide (UV-Titan) in C57BL/6 mice: effects in prenatally exposed offspring on hepatic DNA damage and gene expression

Ole Andersen, Petra Jackson, Sabina Halappanavar, Karin Sørig Hougaard, Andrew Williams, Anne Mette Madsen, Jacob Stuart Lamson, Caroline Yauk, Erik Håkan Richard Wallin, Ulla Birgitte Vogel

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    We investigated effects of maternal pulmonary exposure to titanium dioxide (UV-Titan) on prenatally exposed offspring. Time-mated mice (C57BL/6BomTac) were inhalation exposed (1 h/day to 42 mg UV-Titan/m3 aerosolised powder or filtered air) during gestation days (GDs) 8–18. We evaluated DNA strand breaks using the comet assay in bronchoalveolar lavage (BAL) cells and livers of the time-mated mice (5 and 26–27 days after inhalation exposure), and in livers of the offspring (post-natal days (PND) 2 and 22). We also analysed hepatic gene expression in newborns using DNA microarrays. UV-Titan exposure did not induce DNA strand breaks in time-mated mice or their offspring. Transcriptional profiling of newborn livers revealed changes in the gene expression related to the retinoic acid signalling pathway in the females, while gene expression in male offspring was unaffected. Changes may be a secondary response to maternal inflammation although no direct link was evident through gene expression analysis.
    OriginalsprogEngelsk
    TidsskriftNanotoxicology
    Vol/bind7
    Udgave nummer1
    Sider (fra-til)85-96
    ISSN1743-5390
    DOI
    StatusUdgivet - feb. 2013

    Emneord

    • In utero exposure
    • lung inflammation
    • DNA strand breaks
    • retinoic acid pathway
    • acute-phase response

    Citer dette

    Andersen, Ole ; Jackson, Petra ; Halappanavar, Sabina ; Sørig Hougaard, Karin ; Williams, Andrew ; Madsen, Anne Mette ; Stuart Lamson, Jacob ; Yauk, Caroline ; Wallin, Erik Håkan Richard ; Vogel, Ulla Birgitte. / Maternal inhalation of surface-coated nanosized titanium dioxide (UV-Titan) in C57BL/6 mice: effects in prenatally exposed offspring on hepatic DNA damage and gene expression. I: Nanotoxicology. 2013 ; Bind 7, Nr. 1. s. 85-96.
    @article{43fd65dba94649cf92d8a860315d1c5b,
    title = "Maternal inhalation of surface-coated nanosized titanium dioxide (UV-Titan) in C57BL/6 mice: effects in prenatally exposed offspring on hepatic DNA damage and gene expression",
    abstract = "We investigated effects of maternal pulmonary exposure to titanium dioxide (UV-Titan) on prenatally exposed offspring. Time-mated mice (C57BL/6BomTac) were inhalation exposed (1 h/day to 42 mg UV-Titan/m3 aerosolised powder or filtered air) during gestation days (GDs) 8–18. We evaluated DNA strand breaks using the comet assay in bronchoalveolar lavage (BAL) cells and livers of the time-mated mice (5 and 26–27 days after inhalation exposure), and in livers of the offspring (post-natal days (PND) 2 and 22). We also analysed hepatic gene expression in newborns using DNA microarrays. UV-Titan exposure did not induce DNA strand breaks in time-mated mice or their offspring. Transcriptional profiling of newborn livers revealed changes in the gene expression related to the retinoic acid signalling pathway in the females, while gene expression in male offspring was unaffected. Changes may be a secondary response to maternal inflammation although no direct link was evident through gene expression analysis.",
    keywords = "In utero exposure, lung inflammation, DNA strand breaks, retinoic acid pathway, acute-phase response, In utero exposure, lung inflammation, DNA strand breaks, retinoic acid pathway, acute-phase response",
    author = "Ole Andersen and Petra Jackson and Sabina Halappanavar and {S{\o}rig Hougaard}, Karin and Andrew Williams and Madsen, {Anne Mette} and {Stuart Lamson}, Jacob and Caroline Yauk and Wallin, {Erik H{\aa}kan Richard} and Vogel, {Ulla Birgitte}",
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    month = "2",
    doi = "10.3109/17435390.2011.633715",
    language = "English",
    volume = "7",
    pages = "85--96",
    journal = "Nanotoxicology",
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    Andersen, O, Jackson, P, Halappanavar, S, Sørig Hougaard, K, Williams, A, Madsen, AM, Stuart Lamson, J, Yauk, C, Wallin, EHR & Vogel, UB 2013, 'Maternal inhalation of surface-coated nanosized titanium dioxide (UV-Titan) in C57BL/6 mice: effects in prenatally exposed offspring on hepatic DNA damage and gene expression', Nanotoxicology, bind 7, nr. 1, s. 85-96. https://doi.org/10.3109/17435390.2011.633715

    Maternal inhalation of surface-coated nanosized titanium dioxide (UV-Titan) in C57BL/6 mice: effects in prenatally exposed offspring on hepatic DNA damage and gene expression. / Andersen, Ole; Jackson, Petra; Halappanavar, Sabina; Sørig Hougaard, Karin ; Williams, Andrew; Madsen, Anne Mette ; Stuart Lamson, Jacob ; Yauk, Caroline ; Wallin, Erik Håkan Richard; Vogel, Ulla Birgitte.

    I: Nanotoxicology, Bind 7, Nr. 1, 02.2013, s. 85-96.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Maternal inhalation of surface-coated nanosized titanium dioxide (UV-Titan) in C57BL/6 mice: effects in prenatally exposed offspring on hepatic DNA damage and gene expression

    AU - Andersen, Ole

    AU - Jackson, Petra

    AU - Halappanavar, Sabina

    AU - Sørig Hougaard, Karin

    AU - Williams, Andrew

    AU - Madsen, Anne Mette

    AU - Stuart Lamson, Jacob

    AU - Yauk, Caroline

    AU - Wallin, Erik Håkan Richard

    AU - Vogel, Ulla Birgitte

    PY - 2013/2

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    N2 - We investigated effects of maternal pulmonary exposure to titanium dioxide (UV-Titan) on prenatally exposed offspring. Time-mated mice (C57BL/6BomTac) were inhalation exposed (1 h/day to 42 mg UV-Titan/m3 aerosolised powder or filtered air) during gestation days (GDs) 8–18. We evaluated DNA strand breaks using the comet assay in bronchoalveolar lavage (BAL) cells and livers of the time-mated mice (5 and 26–27 days after inhalation exposure), and in livers of the offspring (post-natal days (PND) 2 and 22). We also analysed hepatic gene expression in newborns using DNA microarrays. UV-Titan exposure did not induce DNA strand breaks in time-mated mice or their offspring. Transcriptional profiling of newborn livers revealed changes in the gene expression related to the retinoic acid signalling pathway in the females, while gene expression in male offspring was unaffected. Changes may be a secondary response to maternal inflammation although no direct link was evident through gene expression analysis.

    AB - We investigated effects of maternal pulmonary exposure to titanium dioxide (UV-Titan) on prenatally exposed offspring. Time-mated mice (C57BL/6BomTac) were inhalation exposed (1 h/day to 42 mg UV-Titan/m3 aerosolised powder or filtered air) during gestation days (GDs) 8–18. We evaluated DNA strand breaks using the comet assay in bronchoalveolar lavage (BAL) cells and livers of the time-mated mice (5 and 26–27 days after inhalation exposure), and in livers of the offspring (post-natal days (PND) 2 and 22). We also analysed hepatic gene expression in newborns using DNA microarrays. UV-Titan exposure did not induce DNA strand breaks in time-mated mice or their offspring. Transcriptional profiling of newborn livers revealed changes in the gene expression related to the retinoic acid signalling pathway in the females, while gene expression in male offspring was unaffected. Changes may be a secondary response to maternal inflammation although no direct link was evident through gene expression analysis.

    KW - In utero exposure

    KW - lung inflammation

    KW - DNA strand breaks

    KW - retinoic acid pathway

    KW - acute-phase response

    KW - In utero exposure

    KW - lung inflammation

    KW - DNA strand breaks

    KW - retinoic acid pathway

    KW - acute-phase response

    U2 - 10.3109/17435390.2011.633715

    DO - 10.3109/17435390.2011.633715

    M3 - Journal article

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    SP - 85

    EP - 96

    JO - Nanotoxicology

    JF - Nanotoxicology

    SN - 1743-5390

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    ER -