Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites

Selwyn Quan, Ole Skovgaard, Robert E McLaughlin, Ed T Buurman, Catherine L Squires

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Single-copy rrn strains facilitate genetic ribosomal studies in Escherichia coli. Consecutive markerless deletion of rrn operons resulted in slower growth upon inactivation of the fourth copy, which was reversed by supplying transfer RNA genes encoded in rrn operons in trans. Removal of the sixth, penultimate rrn copy led to a reduced growth rate due to limited rrn gene dosage. Whole-genome sequencing of variants of single-copy rrn strains revealed duplications of large stretches of genomic DNA. The combination of selective pressure, resulting from the decreased growth rate, and the six identical remaining scar sequences, facilitating homologous recombination events, presumably leads to elevated genomic instability.
    OriginalsprogEngelsk
    TidsskriftG3: Genes, Genomes, Genetics
    Vol/bind5
    Udgave nummer12
    Sider (fra-til)2255-2257
    Antal sider3
    ISSN2160-1836
    DOI
    StatusUdgivet - 2015

    Citer dette

    Quan, Selwyn ; Skovgaard, Ole ; McLaughlin, Robert E ; Buurman, Ed T ; Squires, Catherine L. / Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites. I: G3: Genes, Genomes, Genetics. 2015 ; Bind 5, Nr. 12. s. 2255-2257.
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    title = "Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites",
    abstract = "Single-copy rrn strains facilitate genetic ribosomal studies in Escherichia coli. Consecutive markerless deletion of rrn operons resulted in slower growth upon inactivation of the fourth copy, which was reversed by supplying transfer RNA genes encoded in rrn operons in trans. Removal of the sixth, penultimate rrn copy led to a reduced growth rate due to limited rrn gene dosage. Whole-genome sequencing of variants of single-copy rrn strains revealed duplications of large stretches of genomic DNA. The combination of selective pressure, resulting from the decreased growth rate, and the six identical remaining scar sequences, facilitating homologous recombination events, presumably leads to elevated genomic instability",
    author = "Selwyn Quan and Ole Skovgaard and McLaughlin, {Robert E} and Buurman, {Ed T} and Squires, {Catherine L}",
    year = "2015",
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    Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites. / Quan, Selwyn; Skovgaard, Ole; McLaughlin, Robert E; Buurman, Ed T; Squires, Catherine L.

    I: G3: Genes, Genomes, Genetics, Bind 5, Nr. 12, 2015, s. 2255-2257.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites

    AU - Quan, Selwyn

    AU - Skovgaard, Ole

    AU - McLaughlin, Robert E

    AU - Buurman, Ed T

    AU - Squires, Catherine L

    PY - 2015

    Y1 - 2015

    N2 - Single-copy rrn strains facilitate genetic ribosomal studies in Escherichia coli. Consecutive markerless deletion of rrn operons resulted in slower growth upon inactivation of the fourth copy, which was reversed by supplying transfer RNA genes encoded in rrn operons in trans. Removal of the sixth, penultimate rrn copy led to a reduced growth rate due to limited rrn gene dosage. Whole-genome sequencing of variants of single-copy rrn strains revealed duplications of large stretches of genomic DNA. The combination of selective pressure, resulting from the decreased growth rate, and the six identical remaining scar sequences, facilitating homologous recombination events, presumably leads to elevated genomic instability

    AB - Single-copy rrn strains facilitate genetic ribosomal studies in Escherichia coli. Consecutive markerless deletion of rrn operons resulted in slower growth upon inactivation of the fourth copy, which was reversed by supplying transfer RNA genes encoded in rrn operons in trans. Removal of the sixth, penultimate rrn copy led to a reduced growth rate due to limited rrn gene dosage. Whole-genome sequencing of variants of single-copy rrn strains revealed duplications of large stretches of genomic DNA. The combination of selective pressure, resulting from the decreased growth rate, and the six identical remaining scar sequences, facilitating homologous recombination events, presumably leads to elevated genomic instability

    U2 - doi:10.1534/g3.115.022301

    DO - doi:10.1534/g3.115.022301

    M3 - Journal article

    VL - 5

    SP - 2255

    EP - 2257

    JO - G3: Genes, Genomes, Genetics

    JF - G3: Genes, Genomes, Genetics

    SN - 2160-1836

    IS - 12

    ER -