Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites

Selwyn Quan, Ole Skovgaard, Robert E McLaughlin, Ed T Buurman, Catherine L Squires

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Abstract

    Single-copy rrn strains facilitate genetic ribosomal studies in Escherichia coli. Consecutive markerless deletion of rrn operons resulted in slower growth upon inactivation of the fourth copy, which was reversed by supplying transfer RNA genes encoded in rrn operons in trans. Removal of the sixth, penultimate rrn copy led to a reduced growth rate due to limited rrn gene dosage. Whole-genome sequencing of variants of single-copy rrn strains revealed duplications of large stretches of genomic DNA. The combination of selective pressure, resulting from the decreased growth rate, and the six identical remaining scar sequences, facilitating homologous recombination events, presumably leads to elevated genomic instability.
    OriginalsprogEngelsk
    TidsskriftG3: Genes, Genomes, Genetics
    Vol/bind5
    Udgave nummer12
    Sider (fra-til)2255-2257
    Antal sider3
    ISSN2160-1836
    DOI
    StatusUdgivet - 2015

    Citer dette