Abstract
MicroRNAs (miRNAs) are elements of the gene regulatory network and manipulating their abundance is essential toward elucidating their role in patho-physiological conditions. We present a detailed workflow that identifies important miRNAs using a machine learning algorithm. We then provide optimized techniques to validate the identified miRNAs through over-expression/loss-of-function studies. Overall, these protocols apply to any field in biology where high-dimensional data are produced. For complete details on the use and execution of this protocol, please refer to Wong et al. (2021a).
Originalsprog | Engelsk |
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Artikelnummer | 100910 |
Tidsskrift | STAR protocols |
Vol/bind | 2 |
Udgave nummer | 4 |
ISSN | 2666-1667 |
DOI | |
Status | Udgivet - 17 dec. 2021 |