Localization of microRNA-375 in perinatal rat pancreas

Louise Larsen, Maiken Worsøe Rosenstierne, Jens Høiriis Nielsen, Louise Torp Dalgaard

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

MicroRNA (miRNA)-375 is highly expressed in the pancreatic endocrine islets. Maintaining appropriate miR-375 levels is very important for beta cell development, function and proliferation. The aim of the current study
was to investigate the regulation and localization of miR-375 in rat perinatal pancreas at embryonic day 20 (E20), postnatal day 0 (D0) and day 2 (D2). Expression levels of miR-375 were measured by in situ hybridization
on fixed neonatal rat pancreas. Interestingly, while miR-375 was detectable at robust levels at all three time points, the major site of expression of miR-375 at D0 and D2 was in pancreatic exocrine cells. Our data show that miR-375 has a dynamic change of expression in pancreatic exocrine tissue during the perinatal period. Moreover, these findings indicate that pancreatic endocrine cells
may not always be the major source of expression of miR-375 in pancreas. We suggest that the marked change of miR-375 levels in exocrine cells following birth could regulate processes involved in the adaptation of the exocrine pancreas to digestion of external nutrients derived from milk.
OriginalsprogEngelsk
TidsskriftNon-Coding RNAs in Endocrinology
Vol/bind3
Udgave nummer1
Antal sider4
ISSN2300-4258
DOI
StatusUdgivet - 2018

Citer dette

Larsen, Louise ; Rosenstierne, Maiken Worsøe ; Nielsen, Jens Høiriis ; Dalgaard, Louise Torp. / Localization of microRNA-375 in perinatal rat pancreas. I: Non-Coding RNAs in Endocrinology. 2018 ; Bind 3, Nr. 1.
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title = "Localization of microRNA-375 in perinatal rat pancreas",
abstract = "MicroRNA (miRNA)-375 is highly expressed in the pancreatic endocrine islets. Maintaining appropriate miR-375 levels is very important for beta cell development, function and proliferation. The aim of the current studywas to investigate the regulation and localization of miR-375 in rat perinatal pancreas at embryonic day 20 (E20), postnatal day 0 (D0) and day 2 (D2). Expression levels of miR-375 were measured by in situ hybridizationon fixed neonatal rat pancreas. Interestingly, while miR-375 was detectable at robust levels at all three time points, the major site of expression of miR-375 at D0 and D2 was in pancreatic exocrine cells. Our data show that miR-375 has a dynamic change of expression in pancreatic exocrine tissue during the perinatal period. Moreover, these findings indicate that pancreatic endocrine cellsmay not always be the major source of expression of miR-375 in pancreas. We suggest that the marked change of miR-375 levels in exocrine cells following birth could regulate processes involved in the adaptation of the exocrine pancreas to digestion of external nutrients derived from milk.",
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Localization of microRNA-375 in perinatal rat pancreas. / Larsen, Louise; Rosenstierne, Maiken Worsøe; Nielsen, Jens Høiriis; Dalgaard, Louise Torp.

I: Non-Coding RNAs in Endocrinology, Bind 3, Nr. 1, 2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Localization of microRNA-375 in perinatal rat pancreas

AU - Larsen, Louise

AU - Rosenstierne, Maiken Worsøe

AU - Nielsen, Jens Høiriis

AU - Dalgaard, Louise Torp

PY - 2018

Y1 - 2018

N2 - MicroRNA (miRNA)-375 is highly expressed in the pancreatic endocrine islets. Maintaining appropriate miR-375 levels is very important for beta cell development, function and proliferation. The aim of the current studywas to investigate the regulation and localization of miR-375 in rat perinatal pancreas at embryonic day 20 (E20), postnatal day 0 (D0) and day 2 (D2). Expression levels of miR-375 were measured by in situ hybridizationon fixed neonatal rat pancreas. Interestingly, while miR-375 was detectable at robust levels at all three time points, the major site of expression of miR-375 at D0 and D2 was in pancreatic exocrine cells. Our data show that miR-375 has a dynamic change of expression in pancreatic exocrine tissue during the perinatal period. Moreover, these findings indicate that pancreatic endocrine cellsmay not always be the major source of expression of miR-375 in pancreas. We suggest that the marked change of miR-375 levels in exocrine cells following birth could regulate processes involved in the adaptation of the exocrine pancreas to digestion of external nutrients derived from milk.

AB - MicroRNA (miRNA)-375 is highly expressed in the pancreatic endocrine islets. Maintaining appropriate miR-375 levels is very important for beta cell development, function and proliferation. The aim of the current studywas to investigate the regulation and localization of miR-375 in rat perinatal pancreas at embryonic day 20 (E20), postnatal day 0 (D0) and day 2 (D2). Expression levels of miR-375 were measured by in situ hybridizationon fixed neonatal rat pancreas. Interestingly, while miR-375 was detectable at robust levels at all three time points, the major site of expression of miR-375 at D0 and D2 was in pancreatic exocrine cells. Our data show that miR-375 has a dynamic change of expression in pancreatic exocrine tissue during the perinatal period. Moreover, these findings indicate that pancreatic endocrine cellsmay not always be the major source of expression of miR-375 in pancreas. We suggest that the marked change of miR-375 levels in exocrine cells following birth could regulate processes involved in the adaptation of the exocrine pancreas to digestion of external nutrients derived from milk.

U2 - 10.1515/micrnado-2018-0001

DO - 10.1515/micrnado-2018-0001

M3 - Journal article

VL - 3

JO - Non-Coding RNAs in Endocrinology

JF - Non-Coding RNAs in Endocrinology

SN - 2300-4258

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ER -