TY - JOUR
T1 - Levels of Circulating miR-122 are Associated with Weight Loss and Metabolic Syndrome
AU - Hess, Anne Lundby
AU - Larsen, Lesli Hingstrup
AU - Bækgaard Udesen, Pernille
AU - Sanz, Yolanda
AU - Larsen, Thomas Meinert
AU - Dalgaard, Louise Torp
PY - 2020
Y1 - 2020
N2 - Objective
This study investigated whether the levels of specific serum microRNAs (miRNAs) were altered following diet‐induced weight loss and whether the serum miRNAs differed in the presence of the metabolic syndrome.
Methods
The study was a weight loss intervention trial with a prescribed energy deficit of approximately 500 kcal/d. Levels of 22 miRNAs were determined in serum samples from 85 participants with overweight or obesity. miRNAs were analyzed using TaqMan Array miRNA Cards and normalized to the geometric mean of spiked‐in ath‐miR‐159a and U6 small nuclear RNA using the ΔCT method.
Results
The average weight loss was 5.7 kg (P < 0.001). miR‐122‐5p (−0.18 ± 0.06 log fold relative to initial, P < 0.01) and miR‐193a‐5p (−0.12 ± 0.04, P < 0.01) levels decreased in response to weight loss. miR‐126a‐3p (0.11 ± 0.04, P = 0.01) and miR‐222‐3p (1.51 ± 0.12, P < 0.001) levels increased. Furthermore, a higher level of miR‐122‐5p was observed at baseline in participants with the metabolic syndrome compared with participants without (0.28 ± 0.08, P < 0.01).
Conclusions
Changes in circulating miR‐122‐5p, miR‐126a‐3p, miR‐193a‐5p, and miR‐222‐3p in response to diet‐induced weight loss are demonstrated. Furthermore, assessment of miR‐122‐5p could be an indicator of an adverse metabolic health status independent of obesity.
AB - Objective
This study investigated whether the levels of specific serum microRNAs (miRNAs) were altered following diet‐induced weight loss and whether the serum miRNAs differed in the presence of the metabolic syndrome.
Methods
The study was a weight loss intervention trial with a prescribed energy deficit of approximately 500 kcal/d. Levels of 22 miRNAs were determined in serum samples from 85 participants with overweight or obesity. miRNAs were analyzed using TaqMan Array miRNA Cards and normalized to the geometric mean of spiked‐in ath‐miR‐159a and U6 small nuclear RNA using the ΔCT method.
Results
The average weight loss was 5.7 kg (P < 0.001). miR‐122‐5p (−0.18 ± 0.06 log fold relative to initial, P < 0.01) and miR‐193a‐5p (−0.12 ± 0.04, P < 0.01) levels decreased in response to weight loss. miR‐126a‐3p (0.11 ± 0.04, P = 0.01) and miR‐222‐3p (1.51 ± 0.12, P < 0.001) levels increased. Furthermore, a higher level of miR‐122‐5p was observed at baseline in participants with the metabolic syndrome compared with participants without (0.28 ± 0.08, P < 0.01).
Conclusions
Changes in circulating miR‐122‐5p, miR‐126a‐3p, miR‐193a‐5p, and miR‐222‐3p in response to diet‐induced weight loss are demonstrated. Furthermore, assessment of miR‐122‐5p could be an indicator of an adverse metabolic health status independent of obesity.
U2 - 10.1002/oby.22704
DO - 10.1002/oby.22704
M3 - Journal article
VL - 28
SP - 493
EP - 501
JO - Obesity
JF - Obesity
SN - 1930-7381
IS - 3
ER -