The efficient bioactivation of CO2provides an alternative new strategy for producing high value chemicals and fuels. Normally,in vitroactivation of CO2can be achieved by using formate dehydrogenase (FDH). However, the CO2solubility and the activity of commercial FDHCb remain a tough challenge for the efficient bioactivation of CO2. Here, we report a new “ionozyme” strategy created by using ionic liquids (ILs) as a solvent and enzyme stabilizer, resulting in a 142.3-fold increase in CO2conversion over FDHCb. The ionozyme (FDHPa-[CH][Pro]-[TMGH][PhO]) was first developed by combing a discovered novel FDHPa with an efficient synergistic ionic microenvironment. The remarkable performance of this ionozyme is attributed to forming key intermediates [CH][ProCOO] and [TMGH][PhOCOO], stabilizing the enzyme structure with increased solvation structure, and shortening the distance (3.9 Å) between NADH and CO2to favor the hydride transfer by facilitating their relative orientation and forming new hydrogen bonds at the active sites. This bioactivation of CO2by this specific ionozyme represents ideal starting points for the sustainable carbon cycle.
|Status||Udgivet - 21 sep. 2021|
Bibliografisk noteFunding Information:
This work was supported by the Dalian National Laboratory for Clean Energy (DNL) Cooperation Fund, CAS (DNL201909), the Natural Science Foundation of Beijing (2204097), the Innovation Academy for Green Manufacture, CAS (IAGM2020C19), and the CAS Pioneer Hundred Program.