Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community

Lea Ellegaard-Jensen, Henriette Selck, A Prieme, Daniel Salvito, Valery E. Forbes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The fate of the fragrance material, acetyl cedrene (AC), in sediment was examined in a 16 day laboratory experiment using the sediment microbial community subjected to the following treatments: AC (nominal concentration; 0 and 50 microg g(-1) dw sediment) and macrofaunal worms (with/without Capitella teleta (formerly Capitella sp. I)). Furthermore effects of AC on microbial respiration in the system were determined by examining CO(2) flux. T-RFLP (terminal restriction fragment length polymorphism) was used to analyze PCR (polymerase chain reaction) amplified 16S DNA gene fragments from the sediments to detect changes in the structure and diversity of the bacterial community. In addition, survival of C. teleta in sediment was determined. Lastly, we examined how the interactions between microbes and C. teleta in the sediment affected the above-mentioned parameters. The results showed that there was an interaction between worm treatment and time of sampling on the loss of AC from the sediment. This was caused by AC loss initially being fastest in the sediment with C. teleta present, but at experimental termination there was no significant difference between the two treatments (i.e., with/without worms) in the amount of AC remaining in the sediment. Survival of C. teleta was significantly reduced by AC at experimental termination, but neither microbial respiration nor structure and diversity of the bacterial community were significantly affected
OriginalsprogEngelsk
TidsskriftEcotoxicology
Vol/bind19
Udgave nummer6
Sider (fra-til)1046-1058
Antal sider13
ISSN0963-9292
DOI
StatusUdgivet - 2010

Citer dette

Ellegaard-Jensen, Lea ; Selck, Henriette ; Prieme, A ; Salvito, Daniel ; Forbes, Valery E. / Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community. I: Ecotoxicology. 2010 ; Bind 19, Nr. 6. s. 1046-1058.
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title = "Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community",
abstract = "The fate of the fragrance material, acetyl cedrene (AC), in sediment was examined in a 16 day laboratory experiment using the sediment microbial community subjected to the following treatments: AC (nominal concentration; 0 and 50 microg g(-1) dw sediment) and macrofaunal worms (with/without Capitella teleta (formerly Capitella sp. I)). Furthermore effects of AC on microbial respiration in the system were determined by examining CO(2) flux. T-RFLP (terminal restriction fragment length polymorphism) was used to analyze PCR (polymerase chain reaction) amplified 16S DNA gene fragments from the sediments to detect changes in the structure and diversity of the bacterial community. In addition, survival of C. teleta in sediment was determined. Lastly, we examined how the interactions between microbes and C. teleta in the sediment affected the above-mentioned parameters. The results showed that there was an interaction between worm treatment and time of sampling on the loss of AC from the sediment. This was caused by AC loss initially being fastest in the sediment with C. teleta present, but at experimental termination there was no significant difference between the two treatments (i.e., with/without worms) in the amount of AC remaining in the sediment. Survival of C. teleta was significantly reduced by AC at experimental termination, but neither microbial respiration nor structure and diversity of the bacterial community were significantly affected",
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author = "Lea Ellegaard-Jensen and Henriette Selck and A Prieme and Daniel Salvito and Forbes, {Valery E.}",
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Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community. / Ellegaard-Jensen, Lea; Selck, Henriette; Prieme, A; Salvito, Daniel; Forbes, Valery E.

I: Ecotoxicology, Bind 19, Nr. 6, 2010, s. 1046-1058.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community

AU - Ellegaard-Jensen, Lea

AU - Selck, Henriette

AU - Prieme, A

AU - Salvito, Daniel

AU - Forbes, Valery E.

PY - 2010

Y1 - 2010

N2 - The fate of the fragrance material, acetyl cedrene (AC), in sediment was examined in a 16 day laboratory experiment using the sediment microbial community subjected to the following treatments: AC (nominal concentration; 0 and 50 microg g(-1) dw sediment) and macrofaunal worms (with/without Capitella teleta (formerly Capitella sp. I)). Furthermore effects of AC on microbial respiration in the system were determined by examining CO(2) flux. T-RFLP (terminal restriction fragment length polymorphism) was used to analyze PCR (polymerase chain reaction) amplified 16S DNA gene fragments from the sediments to detect changes in the structure and diversity of the bacterial community. In addition, survival of C. teleta in sediment was determined. Lastly, we examined how the interactions between microbes and C. teleta in the sediment affected the above-mentioned parameters. The results showed that there was an interaction between worm treatment and time of sampling on the loss of AC from the sediment. This was caused by AC loss initially being fastest in the sediment with C. teleta present, but at experimental termination there was no significant difference between the two treatments (i.e., with/without worms) in the amount of AC remaining in the sediment. Survival of C. teleta was significantly reduced by AC at experimental termination, but neither microbial respiration nor structure and diversity of the bacterial community were significantly affected

AB - The fate of the fragrance material, acetyl cedrene (AC), in sediment was examined in a 16 day laboratory experiment using the sediment microbial community subjected to the following treatments: AC (nominal concentration; 0 and 50 microg g(-1) dw sediment) and macrofaunal worms (with/without Capitella teleta (formerly Capitella sp. I)). Furthermore effects of AC on microbial respiration in the system were determined by examining CO(2) flux. T-RFLP (terminal restriction fragment length polymorphism) was used to analyze PCR (polymerase chain reaction) amplified 16S DNA gene fragments from the sediments to detect changes in the structure and diversity of the bacterial community. In addition, survival of C. teleta in sediment was determined. Lastly, we examined how the interactions between microbes and C. teleta in the sediment affected the above-mentioned parameters. The results showed that there was an interaction between worm treatment and time of sampling on the loss of AC from the sediment. This was caused by AC loss initially being fastest in the sediment with C. teleta present, but at experimental termination there was no significant difference between the two treatments (i.e., with/without worms) in the amount of AC remaining in the sediment. Survival of C. teleta was significantly reduced by AC at experimental termination, but neither microbial respiration nor structure and diversity of the bacterial community were significantly affected

KW - ecotoxicology

KW - fragrance materials

KW - sediment

U2 - 10.1007/s10646-010-0486-z

DO - 10.1007/s10646-010-0486-z

M3 - Journal article

VL - 19

SP - 1046

EP - 1058

JO - Ecotoxicology

JF - Ecotoxicology

SN - 0963-9292

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ER -