Investigation of peel and leaf essential oils of Citrus clementina Hort. ex Tan. growing in the south of Vietnam

Thao-Tran Thi Nguyen, Thanh-Tram Thi Tran, Tra-My Hua, Tai The Diep, Duy-Khiem Nguyen Chau, Fritz Duus, Thach Ngoc Le

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The essential oils from peels and leaves of Citrus clementine Hort. ex Tan. were extracted via two methods: conventional hydrodistillation (CHD) and microwave-assisted hydrodistillation (MAHD). Their physicochemical properties were investigated. Their chemical compositions of the oils were determined by GC/FID and GC/MS. A total of forty-one and seventy-seven compounds of the total essential oil composition of the peels and leaves respectively were identified. The peel oils were dominated by monoterpene hydrocarbons in which limonene was the main component (95.48% [CHD], and 95.03% [MAHD]). The leaf oils were also dominated by sesquiterpene hydrocarbons with sabinene (26.02% [CHD], and 19.52% [MAHD]), β-elemene (10.50% [CHD], and 18.04% [MAHD]), linalool (9.88% [CHD], and 7.51% [MAHD]), (E)-β-ocimene (5.87% [CHD], and 5.00% [MAHD]), β-caryophyllene (4.04% [CHD], and 7.49% [MAHD]), and δ-selinene (4.76% [CHD], and 8.02% [MAHD]) as major components. The oil contents of peels were obtained with 5.31% and 5.67% by CHD method and MAHD method, respectively. The oil contents of the leaves were obtained with 0.33% and 0.20% by CHD method and MAHD method, respectively. The antimicrobial activity (disc diffusion antibiotic sensitivity assay) of the oils was investigated against six reference test organisms including two Gram (+) bacteria (Bacillus subtilis, Staphylococcus aureus), three Gram (-) bacteria (Escherichia coli, Pseudomonas aeruginosa, Shigella flexneri), and one fungus (Candida albicans).
OriginalsprogEngelsk
TidsskriftJournal of Essential Oil Research
Vol/bind28
Udgave nummer2
Sider (fra-til)96-103
ISSN1041-2905
DOI
StatusUdgivet - 2016

Emneord

  • Clementine peel and leaf oils
  • Citrus clementina Hort. ex Tan
  • limonene
  • sabinene
  • β-elemene
  • conventional hydrodistillation
  • microwave-assisted hydrodistillation

Citer dette

Nguyen, Thao-Tran Thi ; Thi Tran, Thanh-Tram ; Hua, Tra-My ; Diep, Tai The ; Chau, Duy-Khiem Nguyen ; Duus, Fritz ; Le, Thach Ngoc. / Investigation of peel and leaf essential oils of Citrus clementina Hort. ex Tan. growing in the south of Vietnam. I: Journal of Essential Oil Research. 2016 ; Bind 28, Nr. 2. s. 96-103.
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title = "Investigation of peel and leaf essential oils of Citrus clementina Hort. ex Tan. growing in the south of Vietnam",
abstract = "The essential oils from peels and leaves of Citrus clementine Hort. ex Tan. were extracted via two methods: conventional hydrodistillation (CHD) and microwave-assisted hydrodistillation (MAHD). Their physicochemical properties were investigated. Their chemical compositions of the oils were determined by GC/FID and GC/MS. A total of forty-one and seventy-seven compounds of the total essential oil composition of the peels and leaves respectively were identified. The peel oils were dominated by monoterpene hydrocarbons in which limonene was the main component (95.48{\%} [CHD], and 95.03{\%} [MAHD]). The leaf oils were also dominated by sesquiterpene hydrocarbons with sabinene (26.02{\%} [CHD], and 19.52{\%} [MAHD]), β-elemene (10.50{\%} [CHD], and 18.04{\%} [MAHD]), linalool (9.88{\%} [CHD], and 7.51{\%} [MAHD]), (E)-β-ocimene (5.87{\%} [CHD], and 5.00{\%} [MAHD]), β-caryophyllene (4.04{\%} [CHD], and 7.49{\%} [MAHD]), and δ-selinene (4.76{\%} [CHD], and 8.02{\%} [MAHD]) as major components. The oil contents of peels were obtained with 5.31{\%} and 5.67{\%} by CHD method and MAHD method, respectively. The oil contents of the leaves were obtained with 0.33{\%} and 0.20{\%} by CHD method and MAHD method, respectively. The antimicrobial activity (disc diffusion antibiotic sensitivity assay) of the oils was investigated against six reference test organisms including two Gram (+) bacteria (Bacillus subtilis, Staphylococcus aureus), three Gram (-) bacteria (Escherichia coli, Pseudomonas aeruginosa, Shigella flexneri), and one fungus (Candida albicans).",
keywords = "Clementine peel and leaf oils, Citrus clementina Hort. ex Tan, limonene, sabinene, β-elemene, conventional hydrodistillation, microwave-assisted hydrodistillation",
author = "Nguyen, {Thao-Tran Thi} and {Thi Tran}, Thanh-Tram and Tra-My Hua and Diep, {Tai The} and Chau, {Duy-Khiem Nguyen} and Fritz Duus and Le, {Thach Ngoc}",
year = "2016",
doi = "10.1080/10412905.2015.1083491",
language = "English",
volume = "28",
pages = "96--103",
journal = "Journal of Essential Oil Research",
issn = "1041-2905",
publisher = "Taylor & Francis Inc.",
number = "2",

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Investigation of peel and leaf essential oils of Citrus clementina Hort. ex Tan. growing in the south of Vietnam. / Nguyen, Thao-Tran Thi; Thi Tran, Thanh-Tram; Hua, Tra-My; Diep, Tai The; Chau, Duy-Khiem Nguyen; Duus, Fritz; Le, Thach Ngoc.

I: Journal of Essential Oil Research, Bind 28, Nr. 2, 2016, s. 96-103.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Investigation of peel and leaf essential oils of Citrus clementina Hort. ex Tan. growing in the south of Vietnam

AU - Nguyen, Thao-Tran Thi

AU - Thi Tran, Thanh-Tram

AU - Hua, Tra-My

AU - Diep, Tai The

AU - Chau, Duy-Khiem Nguyen

AU - Duus, Fritz

AU - Le, Thach Ngoc

PY - 2016

Y1 - 2016

N2 - The essential oils from peels and leaves of Citrus clementine Hort. ex Tan. were extracted via two methods: conventional hydrodistillation (CHD) and microwave-assisted hydrodistillation (MAHD). Their physicochemical properties were investigated. Their chemical compositions of the oils were determined by GC/FID and GC/MS. A total of forty-one and seventy-seven compounds of the total essential oil composition of the peels and leaves respectively were identified. The peel oils were dominated by monoterpene hydrocarbons in which limonene was the main component (95.48% [CHD], and 95.03% [MAHD]). The leaf oils were also dominated by sesquiterpene hydrocarbons with sabinene (26.02% [CHD], and 19.52% [MAHD]), β-elemene (10.50% [CHD], and 18.04% [MAHD]), linalool (9.88% [CHD], and 7.51% [MAHD]), (E)-β-ocimene (5.87% [CHD], and 5.00% [MAHD]), β-caryophyllene (4.04% [CHD], and 7.49% [MAHD]), and δ-selinene (4.76% [CHD], and 8.02% [MAHD]) as major components. The oil contents of peels were obtained with 5.31% and 5.67% by CHD method and MAHD method, respectively. The oil contents of the leaves were obtained with 0.33% and 0.20% by CHD method and MAHD method, respectively. The antimicrobial activity (disc diffusion antibiotic sensitivity assay) of the oils was investigated against six reference test organisms including two Gram (+) bacteria (Bacillus subtilis, Staphylococcus aureus), three Gram (-) bacteria (Escherichia coli, Pseudomonas aeruginosa, Shigella flexneri), and one fungus (Candida albicans).

AB - The essential oils from peels and leaves of Citrus clementine Hort. ex Tan. were extracted via two methods: conventional hydrodistillation (CHD) and microwave-assisted hydrodistillation (MAHD). Their physicochemical properties were investigated. Their chemical compositions of the oils were determined by GC/FID and GC/MS. A total of forty-one and seventy-seven compounds of the total essential oil composition of the peels and leaves respectively were identified. The peel oils were dominated by monoterpene hydrocarbons in which limonene was the main component (95.48% [CHD], and 95.03% [MAHD]). The leaf oils were also dominated by sesquiterpene hydrocarbons with sabinene (26.02% [CHD], and 19.52% [MAHD]), β-elemene (10.50% [CHD], and 18.04% [MAHD]), linalool (9.88% [CHD], and 7.51% [MAHD]), (E)-β-ocimene (5.87% [CHD], and 5.00% [MAHD]), β-caryophyllene (4.04% [CHD], and 7.49% [MAHD]), and δ-selinene (4.76% [CHD], and 8.02% [MAHD]) as major components. The oil contents of peels were obtained with 5.31% and 5.67% by CHD method and MAHD method, respectively. The oil contents of the leaves were obtained with 0.33% and 0.20% by CHD method and MAHD method, respectively. The antimicrobial activity (disc diffusion antibiotic sensitivity assay) of the oils was investigated against six reference test organisms including two Gram (+) bacteria (Bacillus subtilis, Staphylococcus aureus), three Gram (-) bacteria (Escherichia coli, Pseudomonas aeruginosa, Shigella flexneri), and one fungus (Candida albicans).

KW - Clementine peel and leaf oils

KW - Citrus clementina Hort. ex Tan

KW - limonene

KW - sabinene

KW - β-elemene

KW - conventional hydrodistillation

KW - microwave-assisted hydrodistillation

U2 - 10.1080/10412905.2015.1083491

DO - 10.1080/10412905.2015.1083491

M3 - Journal article

VL - 28

SP - 96

EP - 103

JO - Journal of Essential Oil Research

JF - Journal of Essential Oil Research

SN - 1041-2905

IS - 2

ER -