Interaction Free Energies of Eight Sodium Salts and a Phosphatidylcholine Membrane

Chunhua Wang, Ying Ge, John Mortensen, Peter Westh

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Many recent reports have discussed specific effects of anions on the properties of lipid membranes and possible roles of such effects within biochemistry. One key parameter in both theoretical and experimental treatments of membrane–salt interactions is the net affinity, that is, the free energy of interaction. This parameter has been analyzed theoretically and by modeling of experimental data but not directly measured even for simple salt–membrane systems. To provide such information, we have studied the interaction of unilamellar liposomes of dimyristoyl phosphatidylcholine (DMPC) and eight sodium salts by dialysis equilibrium measurements. This method provides model free thermodynamic data and allows investigations in the dilute concentration range where solution nonideality and perturbation of membrane structure is limited. The transfer free energy of DMPC from water to salt solutions, Δμ2,trans, was found to scale proportionally to the salt concentration, m3, in the 0–50 mmolal range, and we suggest that the slope, Δμ2,trans/outm3, represents an experimental salt–membrane interaction potential. The values of Δμ2,trans/outm3 for sodium salts of monovalent anions ranged from +2.2 kJ (mol·molal)−1 for F– to −16.9 kJ (mol·molal)−1 for SCN–, thus confirming a distinct membrane specificity among the anions. For Na2SO4, the interaction was strongly unfavorable with Δμ2,trans/outm3 = +20.6 kJ (mol·molal)−1. The dialysis data were supplemented with calorimetric measurements and analyzed with respect to a two-domain model.
OriginalsprogEngelsk
TidsskriftJournal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical
Vol/bind115
Udgave nummer33
Sider (fra-til)9955-9961
ISSN1520-6106
DOI
StatusUdgivet - 2011

Citer dette

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title = "Interaction Free Energies of Eight Sodium Salts and a Phosphatidylcholine Membrane",
abstract = "Many recent reports have discussed specific effects of anions on the properties of lipid membranes and possible roles of such effects within biochemistry. One key parameter in both theoretical and experimental treatments of membrane–salt interactions is the net affinity, that is, the free energy of interaction. This parameter has been analyzed theoretically and by modeling of experimental data but not directly measured even for simple salt–membrane systems. To provide such information, we have studied the interaction of unilamellar liposomes of dimyristoyl phosphatidylcholine (DMPC) and eight sodium salts by dialysis equilibrium measurements. This method provides model free thermodynamic data and allows investigations in the dilute concentration range where solution nonideality and perturbation of membrane structure is limited. The transfer free energy of DMPC from water to salt solutions, Δμ2,trans, was found to scale proportionally to the salt concentration, m3, in the 0–50 mmolal range, and we suggest that the slope, Δμ2,trans/outm3, represents an experimental salt–membrane interaction potential. The values of Δμ2,trans/outm3 for sodium salts of monovalent anions ranged from +2.2 kJ (mol·molal)−1 for F– to −16.9 kJ (mol·molal)−1 for SCN–, thus confirming a distinct membrane specificity among the anions. For Na2SO4, the interaction was strongly unfavorable with Δμ2,trans/outm3 = +20.6 kJ (mol·molal)−1. The dialysis data were supplemented with calorimetric measurements and analyzed with respect to a two-domain model.",
author = "Chunhua Wang and Ying Ge and John Mortensen and Peter Westh",
year = "2011",
doi = "10.1021/jp112203p",
language = "English",
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journal = "Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical",
issn = "1520-6106",
publisher = "American Chemical Society",
number = "33",

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Interaction Free Energies of Eight Sodium Salts and a Phosphatidylcholine Membrane. / Wang, Chunhua; Ge, Ying; Mortensen, John; Westh, Peter.

I: Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical, Bind 115, Nr. 33, 2011, s. 9955-9961.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Interaction Free Energies of Eight Sodium Salts and a Phosphatidylcholine Membrane

AU - Wang, Chunhua

AU - Ge, Ying

AU - Mortensen, John

AU - Westh, Peter

PY - 2011

Y1 - 2011

N2 - Many recent reports have discussed specific effects of anions on the properties of lipid membranes and possible roles of such effects within biochemistry. One key parameter in both theoretical and experimental treatments of membrane–salt interactions is the net affinity, that is, the free energy of interaction. This parameter has been analyzed theoretically and by modeling of experimental data but not directly measured even for simple salt–membrane systems. To provide such information, we have studied the interaction of unilamellar liposomes of dimyristoyl phosphatidylcholine (DMPC) and eight sodium salts by dialysis equilibrium measurements. This method provides model free thermodynamic data and allows investigations in the dilute concentration range where solution nonideality and perturbation of membrane structure is limited. The transfer free energy of DMPC from water to salt solutions, Δμ2,trans, was found to scale proportionally to the salt concentration, m3, in the 0–50 mmolal range, and we suggest that the slope, Δμ2,trans/outm3, represents an experimental salt–membrane interaction potential. The values of Δμ2,trans/outm3 for sodium salts of monovalent anions ranged from +2.2 kJ (mol·molal)−1 for F– to −16.9 kJ (mol·molal)−1 for SCN–, thus confirming a distinct membrane specificity among the anions. For Na2SO4, the interaction was strongly unfavorable with Δμ2,trans/outm3 = +20.6 kJ (mol·molal)−1. The dialysis data were supplemented with calorimetric measurements and analyzed with respect to a two-domain model.

AB - Many recent reports have discussed specific effects of anions on the properties of lipid membranes and possible roles of such effects within biochemistry. One key parameter in both theoretical and experimental treatments of membrane–salt interactions is the net affinity, that is, the free energy of interaction. This parameter has been analyzed theoretically and by modeling of experimental data but not directly measured even for simple salt–membrane systems. To provide such information, we have studied the interaction of unilamellar liposomes of dimyristoyl phosphatidylcholine (DMPC) and eight sodium salts by dialysis equilibrium measurements. This method provides model free thermodynamic data and allows investigations in the dilute concentration range where solution nonideality and perturbation of membrane structure is limited. The transfer free energy of DMPC from water to salt solutions, Δμ2,trans, was found to scale proportionally to the salt concentration, m3, in the 0–50 mmolal range, and we suggest that the slope, Δμ2,trans/outm3, represents an experimental salt–membrane interaction potential. The values of Δμ2,trans/outm3 for sodium salts of monovalent anions ranged from +2.2 kJ (mol·molal)−1 for F– to −16.9 kJ (mol·molal)−1 for SCN–, thus confirming a distinct membrane specificity among the anions. For Na2SO4, the interaction was strongly unfavorable with Δμ2,trans/outm3 = +20.6 kJ (mol·molal)−1. The dialysis data were supplemented with calorimetric measurements and analyzed with respect to a two-domain model.

U2 - 10.1021/jp112203p

DO - 10.1021/jp112203p

M3 - Journal article

VL - 115

SP - 9955

EP - 9961

JO - Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical

JF - Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical

SN - 1520-6106

IS - 33

ER -