Inhibition of methane hydrate nucleation and growth by an antifreeze protein

Liang Mu, Hans Ramløv, Max Søgaard, Thomas Jørgensen, Willem A. de Jong, Nicolas von Solms

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures. They constitute a class of promising candidates as environmentally kinetic hydrate inhibitors (KHIs). In this study, the effectiveness of an insect cell expressed novel monomeric streptavidin fusion protein version of Rhagium mordax RmAFP1 antifreeze protein (mSA-RmAFP1), and four amino acids (histidine, lysine, tyrosine and proline), on CH4 hydrate nucleation, growth and decomposition was investigated using a rocking cell apparatus, then compared with the commercial inhibitors Polyvinylpyrrolidone (PVP) and Luvicap Bio. It was found that CH4 hydrate nucleation and growth exhibited good repeatable results under experimental conditions. The results showed that 2250 ppm mSA-RmAFP1 can inhibit CH4 hydrate nucleation as effectively as PVP at the same concentration. The histidine, lysine, tyrosine and proline exhibited weak inhibition effect on CH4 hydrate nucleation. The mSA-RmAFP1 decreased CH4 hydrate growth rate and production in the fresh and memory solutions. The CH4 hydrate formed in the solutions containing various tested KHIs present slightly lower onset decomposition temperatures than the non-inhibited system under experimental conditions. The promising performance of the insect cell expressed mSA-RmAFP1 could promote the further development of green hydrate inhibitors. The production of this protein through insect cell line fermentation provides a platform for the future production and optimization of AFPs for hydrate inhibition.
OriginalsprogEngelsk
Artikelnummer106388
TidsskriftJournal of Petroleum Science and Engineering
Vol/bind2019
Udgave nummer183
ISSN0920-4105
DOI
StatusUdgivet - dec. 2019

Emneord

  • Antifreeze protein
  • Methane hydrate
  • inhibition

Citer dette

Mu, Liang ; Ramløv, Hans ; Søgaard, Max ; Jørgensen, Thomas ; de Jong, Willem A. ; von Solms, Nicolas. / Inhibition of methane hydrate nucleation and growth by an antifreeze protein. I: Journal of Petroleum Science and Engineering. 2019 ; Bind 2019, Nr. 183.
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abstract = "Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures. They constitute a class of promising candidates as environmentally kinetic hydrate inhibitors (KHIs). In this study, the effectiveness of an insect cell expressed novel monomeric streptavidin fusion protein version of Rhagium mordax RmAFP1 antifreeze protein (mSA-RmAFP1), and four amino acids (histidine, lysine, tyrosine and proline), on CH4 hydrate nucleation, growth and decomposition was investigated using a rocking cell apparatus, then compared with the commercial inhibitors Polyvinylpyrrolidone (PVP) and Luvicap Bio. It was found that CH4 hydrate nucleation and growth exhibited good repeatable results under experimental conditions. The results showed that 2250 ppm mSA-RmAFP1 can inhibit CH4 hydrate nucleation as effectively as PVP at the same concentration. The histidine, lysine, tyrosine and proline exhibited weak inhibition effect on CH4 hydrate nucleation. The mSA-RmAFP1 decreased CH4 hydrate growth rate and production in the fresh and memory solutions. The CH4 hydrate formed in the solutions containing various tested KHIs present slightly lower onset decomposition temperatures than the non-inhibited system under experimental conditions. The promising performance of the insect cell expressed mSA-RmAFP1 could promote the further development of green hydrate inhibitors. The production of this protein through insect cell line fermentation provides a platform for the future production and optimization of AFPs for hydrate inhibition.",
keywords = "Antifreeze protein, Methane hydrate, inhibition",
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year = "2019",
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Inhibition of methane hydrate nucleation and growth by an antifreeze protein. / Mu, Liang; Ramløv, Hans; Søgaard, Max; Jørgensen, Thomas; de Jong, Willem A.; von Solms, Nicolas.

I: Journal of Petroleum Science and Engineering, Bind 2019, Nr. 183, 106388, 12.2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Inhibition of methane hydrate nucleation and growth by an antifreeze protein

AU - Mu, Liang

AU - Ramløv, Hans

AU - Søgaard, Max

AU - Jørgensen, Thomas

AU - de Jong, Willem A.

AU - von Solms, Nicolas

PY - 2019/12

Y1 - 2019/12

N2 - Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures. They constitute a class of promising candidates as environmentally kinetic hydrate inhibitors (KHIs). In this study, the effectiveness of an insect cell expressed novel monomeric streptavidin fusion protein version of Rhagium mordax RmAFP1 antifreeze protein (mSA-RmAFP1), and four amino acids (histidine, lysine, tyrosine and proline), on CH4 hydrate nucleation, growth and decomposition was investigated using a rocking cell apparatus, then compared with the commercial inhibitors Polyvinylpyrrolidone (PVP) and Luvicap Bio. It was found that CH4 hydrate nucleation and growth exhibited good repeatable results under experimental conditions. The results showed that 2250 ppm mSA-RmAFP1 can inhibit CH4 hydrate nucleation as effectively as PVP at the same concentration. The histidine, lysine, tyrosine and proline exhibited weak inhibition effect on CH4 hydrate nucleation. The mSA-RmAFP1 decreased CH4 hydrate growth rate and production in the fresh and memory solutions. The CH4 hydrate formed in the solutions containing various tested KHIs present slightly lower onset decomposition temperatures than the non-inhibited system under experimental conditions. The promising performance of the insect cell expressed mSA-RmAFP1 could promote the further development of green hydrate inhibitors. The production of this protein through insect cell line fermentation provides a platform for the future production and optimization of AFPs for hydrate inhibition.

AB - Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures. They constitute a class of promising candidates as environmentally kinetic hydrate inhibitors (KHIs). In this study, the effectiveness of an insect cell expressed novel monomeric streptavidin fusion protein version of Rhagium mordax RmAFP1 antifreeze protein (mSA-RmAFP1), and four amino acids (histidine, lysine, tyrosine and proline), on CH4 hydrate nucleation, growth and decomposition was investigated using a rocking cell apparatus, then compared with the commercial inhibitors Polyvinylpyrrolidone (PVP) and Luvicap Bio. It was found that CH4 hydrate nucleation and growth exhibited good repeatable results under experimental conditions. The results showed that 2250 ppm mSA-RmAFP1 can inhibit CH4 hydrate nucleation as effectively as PVP at the same concentration. The histidine, lysine, tyrosine and proline exhibited weak inhibition effect on CH4 hydrate nucleation. The mSA-RmAFP1 decreased CH4 hydrate growth rate and production in the fresh and memory solutions. The CH4 hydrate formed in the solutions containing various tested KHIs present slightly lower onset decomposition temperatures than the non-inhibited system under experimental conditions. The promising performance of the insect cell expressed mSA-RmAFP1 could promote the further development of green hydrate inhibitors. The production of this protein through insect cell line fermentation provides a platform for the future production and optimization of AFPs for hydrate inhibition.

KW - Antifreeze protein

KW - Methane hydrate

KW - inhibition

U2 - 10.1016/j.petrol.2019.106388

DO - 10.1016/j.petrol.2019.106388

M3 - Journal article

VL - 2019

JO - Journal of Petroleum Science and Engineering

JF - Journal of Petroleum Science and Engineering

SN - 0920-4105

IS - 183

M1 - 106388

ER -