Inhibition of Gas Hydrate Nucleation and Growth

Efficacy of an Antifreeze Protein from the Longhorn Beetle Rhagium mordax

Dennis Steven Friis, Erlend Kristiansen, Hans Ramløv, Christine Malmros, Pei Cheng Chua, Nagu Daraboina, Malcolm Kelland, Nicolas von Solms, John Woodley

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures by preventing tiny ice crystals in solution from growing as the solution is cooled below its freezing temperature. This inhibition of ice growth is called antifreeze activity, and in particular, certain insect AFPs show very high antifreeze activity. Recent studies have shown AFPs to be promising candidates as green and environmentally benign inhibitors for gas hydrate formation. Here we show that an insect antifreeze protein from the longhorn beetle, Rhagium mordax (RmAFP1), the most potent protein yet found for freezing inhibition, can inhibit methane hydrates as effectively as the synthetic polymeric inhibitor polyvinylpyrrolidone (PVP). In high pressure rocking cell experiments, onset hydrate nucleation temperatures and growth profiles showed repeatable results. RmAFP1 clearly showed inhibition of hydrates compared to amino acids (l-valine and l-threonine) and the protein bovine serum albumin (BSA). This indicates that proteins or amino acids do not generally inhibit hydrate formation. The promising performance of RmAFP1 as a new green kinetic hydrate inhibitor could further the development and increased production of green hydrate inhibitors.
    OriginalsprogEngelsk
    TidsskriftEnergy & Fuels
    Vol/bind28
    Udgave nummer6
    Sider (fra-til)3666-3672
    Antal sider7
    ISSN0887-0624
    DOI
    StatusUdgivet - 19 jun. 2014

    Citer dette

    Friis, Dennis Steven ; Kristiansen, Erlend ; Ramløv, Hans ; Malmros, Christine ; Chua, Pei Cheng ; Daraboina, Nagu ; Kelland, Malcolm ; von Solms, Nicolas ; Woodley, John. / Inhibition of Gas Hydrate Nucleation and Growth : Efficacy of an Antifreeze Protein from the Longhorn Beetle Rhagium mordax. I: Energy & Fuels. 2014 ; Bind 28, Nr. 6. s. 3666-3672.
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    title = "Inhibition of Gas Hydrate Nucleation and Growth: Efficacy of an Antifreeze Protein from the Longhorn Beetle Rhagium mordax",
    abstract = "Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures by preventing tiny ice crystals in solution from growing as the solution is cooled below its freezing temperature. This inhibition of ice growth is called antifreeze activity, and in particular, certain insect AFPs show very high antifreeze activity. Recent studies have shown AFPs to be promising candidates as green and environmentally benign inhibitors for gas hydrate formation. Here we show that an insect antifreeze protein from the longhorn beetle, Rhagium mordax (RmAFP1), the most potent protein yet found for freezing inhibition, can inhibit methane hydrates as effectively as the synthetic polymeric inhibitor polyvinylpyrrolidone (PVP). In high pressure rocking cell experiments, onset hydrate nucleation temperatures and growth profiles showed repeatable results. RmAFP1 clearly showed inhibition of hydrates compared to amino acids (l-valine and l-threonine) and the protein bovine serum albumin (BSA). This indicates that proteins or amino acids do not generally inhibit hydrate formation. The promising performance of RmAFP1 as a new green kinetic hydrate inhibitor could further the development and increased production of green hydrate inhibitors.",
    author = "Friis, {Dennis Steven} and Erlend Kristiansen and Hans Raml{\o}v and Christine Malmros and Chua, {Pei Cheng} and Nagu Daraboina and Malcolm Kelland and {von Solms}, Nicolas and John Woodley",
    year = "2014",
    month = "6",
    day = "19",
    doi = "10.1021/ef500349w",
    language = "English",
    volume = "28",
    pages = "3666--3672",
    journal = "Energy & Fuels",
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    Friis, DS, Kristiansen, E, Ramløv, H, Malmros, C, Chua, PC, Daraboina, N, Kelland, M, von Solms, N & Woodley, J 2014, 'Inhibition of Gas Hydrate Nucleation and Growth: Efficacy of an Antifreeze Protein from the Longhorn Beetle Rhagium mordax', Energy & Fuels, bind 28, nr. 6, s. 3666-3672. https://doi.org/10.1021/ef500349w

    Inhibition of Gas Hydrate Nucleation and Growth : Efficacy of an Antifreeze Protein from the Longhorn Beetle Rhagium mordax. / Friis, Dennis Steven; Kristiansen, Erlend; Ramløv, Hans; Malmros, Christine; Chua, Pei Cheng; Daraboina, Nagu; Kelland, Malcolm; von Solms, Nicolas; Woodley, John.

    I: Energy & Fuels, Bind 28, Nr. 6, 19.06.2014, s. 3666-3672.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Inhibition of Gas Hydrate Nucleation and Growth

    T2 - Efficacy of an Antifreeze Protein from the Longhorn Beetle Rhagium mordax

    AU - Friis, Dennis Steven

    AU - Kristiansen, Erlend

    AU - Ramløv, Hans

    AU - Malmros, Christine

    AU - Chua, Pei Cheng

    AU - Daraboina, Nagu

    AU - Kelland, Malcolm

    AU - von Solms, Nicolas

    AU - Woodley, John

    PY - 2014/6/19

    Y1 - 2014/6/19

    N2 - Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures by preventing tiny ice crystals in solution from growing as the solution is cooled below its freezing temperature. This inhibition of ice growth is called antifreeze activity, and in particular, certain insect AFPs show very high antifreeze activity. Recent studies have shown AFPs to be promising candidates as green and environmentally benign inhibitors for gas hydrate formation. Here we show that an insect antifreeze protein from the longhorn beetle, Rhagium mordax (RmAFP1), the most potent protein yet found for freezing inhibition, can inhibit methane hydrates as effectively as the synthetic polymeric inhibitor polyvinylpyrrolidone (PVP). In high pressure rocking cell experiments, onset hydrate nucleation temperatures and growth profiles showed repeatable results. RmAFP1 clearly showed inhibition of hydrates compared to amino acids (l-valine and l-threonine) and the protein bovine serum albumin (BSA). This indicates that proteins or amino acids do not generally inhibit hydrate formation. The promising performance of RmAFP1 as a new green kinetic hydrate inhibitor could further the development and increased production of green hydrate inhibitors.

    AB - Antifreeze proteins (AFPs) are characterized by their ability to protect organisms from subfreezing temperatures by preventing tiny ice crystals in solution from growing as the solution is cooled below its freezing temperature. This inhibition of ice growth is called antifreeze activity, and in particular, certain insect AFPs show very high antifreeze activity. Recent studies have shown AFPs to be promising candidates as green and environmentally benign inhibitors for gas hydrate formation. Here we show that an insect antifreeze protein from the longhorn beetle, Rhagium mordax (RmAFP1), the most potent protein yet found for freezing inhibition, can inhibit methane hydrates as effectively as the synthetic polymeric inhibitor polyvinylpyrrolidone (PVP). In high pressure rocking cell experiments, onset hydrate nucleation temperatures and growth profiles showed repeatable results. RmAFP1 clearly showed inhibition of hydrates compared to amino acids (l-valine and l-threonine) and the protein bovine serum albumin (BSA). This indicates that proteins or amino acids do not generally inhibit hydrate formation. The promising performance of RmAFP1 as a new green kinetic hydrate inhibitor could further the development and increased production of green hydrate inhibitors.

    U2 - 10.1021/ef500349w

    DO - 10.1021/ef500349w

    M3 - Journal article

    VL - 28

    SP - 3666

    EP - 3672

    JO - Energy & Fuels

    JF - Energy & Fuels

    SN - 0887-0624

    IS - 6

    ER -