Influence of copper oxide nanoparticle shape on bioaccumulation, cellular internalization and effects in the estuarine sediment-dwelling polychaete, Nereis diversicolor

Amalie Thit, Agnieszka Dybowska, Carsten Købler, Gabrielle M. Kennaway, Henriette Selck

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

CuO nanoparticles (NPs) released into the aquatic environment will likely accumulate in the sediment. Here we synthesized and characterized CuO NPs with different shapes and thus sizes: spheres, rods and spindles. Nereis diversicolor were exposed for 10 days to control sediment or sediment spiked with CuO NPs or aqueous Cu (Cu-Aq, CuCl2) at 7, 70 and 140 mg Cu g1 dw sediment. Cu from all Cu treatments accumulated in worms in a concentration-dependent manner. Only Cu-Aq decreased burrowing, suggesting that worms avoid Cu when added to sediment as Cu-Aq, but not CuO NPs. Transmission Electron Microscopy of gut sections indicated limited presence of CuO NP-like objects in the gut lumen, but evidence on whether accumulated Cu from CuO NP exposure was internalized as particles was not conclusive. Overall, bioavailability and avoidance was not influenced by particle shape or size, whereas Cu form (Cu-Aq vs particulate) and exposure concentration had significant impact
OriginalsprogEngelsk
TidsskriftMarine Environmental Research
Vol/bind111
Sider (fra-til)89-98
ISSN0141-1136
DOI
StatusUdgivet - 2015

Citer dette

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title = "Influence of copper oxide nanoparticle shape on bioaccumulation, cellular internalization and effects in the estuarine sediment-dwelling polychaete, Nereis diversicolor",
abstract = "CuO nanoparticles (NPs) released into the aquatic environment will likely accumulate in the sediment. Here we synthesized and characterized CuO NPs with different shapes and thus sizes: spheres, rods and spindles. Nereis diversicolor were exposed for 10 days to control sediment or sediment spiked with CuO NPs or aqueous Cu (Cu-Aq, CuCl2) at 7, 70 and 140 μg Cu g−1 dw sediment. Cu from all Cu treatments accumulated in worms in a concentration-dependent manner. Only Cu-Aq decreased burrowing, suggesting that worms avoid Cu when added to sediment as Cu-Aq, but not CuO NPs. Transmission Electron Microscopy of gut sections indicated limited presence of CuO NP-like objects in the gut lumen, but evidence on whether accumulated Cu from CuO NP exposure was internalized as particles was not conclusive. Overall, bioavailability and avoidance was not influenced by particle shape or size, whereas Cu form (Cu-Aq vs particulate) and exposure concentration had significant impact",
author = "Amalie Thit and Agnieszka Dybowska and Carsten K{\o}bler and Kennaway, {Gabrielle M.} and Henriette Selck",
year = "2015",
doi = "10.1016/j.marenvres.2015.06.009",
language = "English",
volume = "111",
pages = "89--98",
journal = "Marine Environmental Research",
issn = "0141-1136",
publisher = "Elsevier Ltd",

}

Influence of copper oxide nanoparticle shape on bioaccumulation, cellular internalization and effects in the estuarine sediment-dwelling polychaete, Nereis diversicolor. / Thit, Amalie ; Dybowska, Agnieszka; Købler, Carsten; Kennaway, Gabrielle M. ; Selck, Henriette.

I: Marine Environmental Research, Bind 111, 2015, s. 89-98.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Influence of copper oxide nanoparticle shape on bioaccumulation, cellular internalization and effects in the estuarine sediment-dwelling polychaete, Nereis diversicolor

AU - Thit, Amalie

AU - Dybowska, Agnieszka

AU - Købler, Carsten

AU - Kennaway, Gabrielle M.

AU - Selck, Henriette

PY - 2015

Y1 - 2015

N2 - CuO nanoparticles (NPs) released into the aquatic environment will likely accumulate in the sediment. Here we synthesized and characterized CuO NPs with different shapes and thus sizes: spheres, rods and spindles. Nereis diversicolor were exposed for 10 days to control sediment or sediment spiked with CuO NPs or aqueous Cu (Cu-Aq, CuCl2) at 7, 70 and 140 μg Cu g−1 dw sediment. Cu from all Cu treatments accumulated in worms in a concentration-dependent manner. Only Cu-Aq decreased burrowing, suggesting that worms avoid Cu when added to sediment as Cu-Aq, but not CuO NPs. Transmission Electron Microscopy of gut sections indicated limited presence of CuO NP-like objects in the gut lumen, but evidence on whether accumulated Cu from CuO NP exposure was internalized as particles was not conclusive. Overall, bioavailability and avoidance was not influenced by particle shape or size, whereas Cu form (Cu-Aq vs particulate) and exposure concentration had significant impact

AB - CuO nanoparticles (NPs) released into the aquatic environment will likely accumulate in the sediment. Here we synthesized and characterized CuO NPs with different shapes and thus sizes: spheres, rods and spindles. Nereis diversicolor were exposed for 10 days to control sediment or sediment spiked with CuO NPs or aqueous Cu (Cu-Aq, CuCl2) at 7, 70 and 140 μg Cu g−1 dw sediment. Cu from all Cu treatments accumulated in worms in a concentration-dependent manner. Only Cu-Aq decreased burrowing, suggesting that worms avoid Cu when added to sediment as Cu-Aq, but not CuO NPs. Transmission Electron Microscopy of gut sections indicated limited presence of CuO NP-like objects in the gut lumen, but evidence on whether accumulated Cu from CuO NP exposure was internalized as particles was not conclusive. Overall, bioavailability and avoidance was not influenced by particle shape or size, whereas Cu form (Cu-Aq vs particulate) and exposure concentration had significant impact

U2 - 10.1016/j.marenvres.2015.06.009

DO - 10.1016/j.marenvres.2015.06.009

M3 - Journal article

VL - 111

SP - 89

EP - 98

JO - Marine Environmental Research

JF - Marine Environmental Research

SN - 0141-1136

ER -