In vivo retention of ingested Au NPs by Daphnia magna

No evidence for trans-epithelial alimentary uptake

Farhan R. Khan, Gabrielle M. Kennaway, Marie-Noelle Croteau, Agnieszka Dybowska, Brian Smith, Antonio J. A. Nogueira, Philip S Rainbow, Samuel Luoma, Eugenia Valsami-Jones

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    In vivo studies with Daphnia magna remain inconclusive as to whether engineered nanoparticles (NPs) are internalized into tissues after ingestion. Here we used a three-pronged approach to study the in vivo retention and efflux kinetics of 20 nm citrate stabilized Au NPs ingested by this key aquatic species. Daphnids were exposed to suspended particles (600 μg L−1) for 5 h after which they were depurated for 24 h in clean water containing algae. Light microscopy was used to follow the passage of Au NPs through the gastrointestinal tract, Au body burdens were determined by ICP-MS (inductively coupled plasma mass spectrometry), and transmission electron microscopy (TEM) was used to examine the presence and distribution of Au NPs in tissues. Results revealed that the elimination of Au NPs was bi-phasic. The fast elimination phase lasted <1 h and the rate constant at which Au (of Au NPs) was eliminated was 1.12 ± 0.34 h−1 (±SE) which accounted for ∼75% of the ingested Au. The remaining ∼25% of the ingested Au NPs was eliminated at a 100-fold slower rate. TEM analysis revealed that Au NPs in the midgut were in close proximity to the peritrophic membrane after 1 and 24 h of depuration. There were no observations of Au NP uptake at the microvilli. Thus, although Au NPs were retained in the gut lumen, there was no observable internalization into the gut epithelial cells. Similar to carbon nanotubes and CuO NPs, our findings indicate that in daphnids the in vivo retention of Au NPs does not necessarily result in their internalization
    OriginalsprogEngelsk
    TidsskriftChemosphere
    Vol/bind100
    Sider (fra-til)97-104
    ISSN0045-6535
    DOI
    StatusUdgivet - 2014

    Citer dette

    Khan, F. R., Kennaway, G. M., Croteau, M-N., Dybowska, A., Smith, B., Nogueira, A. J. A., ... Valsami-Jones, E. (2014). In vivo retention of ingested Au NPs by Daphnia magna: No evidence for trans-epithelial alimentary uptake. Chemosphere, 100, 97-104. https://doi.org/10.1016/j.chemosphere.2013.12.051
    Khan, Farhan R. ; Kennaway, Gabrielle M. ; Croteau, Marie-Noelle ; Dybowska, Agnieszka ; Smith, Brian ; Nogueira, Antonio J. A. ; Rainbow, Philip S ; Luoma, Samuel ; Valsami-Jones, Eugenia. / In vivo retention of ingested Au NPs by Daphnia magna : No evidence for trans-epithelial alimentary uptake. I: Chemosphere. 2014 ; Bind 100. s. 97-104.
    @article{972b1f16c6af4cdb8ce0c534246962d6,
    title = "In vivo retention of ingested Au NPs by Daphnia magna: No evidence for trans-epithelial alimentary uptake",
    abstract = "In vivo studies with Daphnia magna remain inconclusive as to whether engineered nanoparticles (NPs) are internalized into tissues after ingestion. Here we used a three-pronged approach to study the in vivo retention and efflux kinetics of 20 nm citrate stabilized Au NPs ingested by this key aquatic species. Daphnids were exposed to suspended particles (600 μg L−1) for 5 h after which they were depurated for 24 h in clean water containing algae. Light microscopy was used to follow the passage of Au NPs through the gastrointestinal tract, Au body burdens were determined by ICP-MS (inductively coupled plasma mass spectrometry), and transmission electron microscopy (TEM) was used to examine the presence and distribution of Au NPs in tissues. Results revealed that the elimination of Au NPs was bi-phasic. The fast elimination phase lasted <1 h and the rate constant at which Au (of Au NPs) was eliminated was 1.12 ± 0.34 h−1 (±SE) which accounted for ∼75{\%} of the ingested Au. The remaining ∼25{\%} of the ingested Au NPs was eliminated at a 100-fold slower rate. TEM analysis revealed that Au NPs in the midgut were in close proximity to the peritrophic membrane after 1 and 24 h of depuration. There were no observations of Au NP uptake at the microvilli. Thus, although Au NPs were retained in the gut lumen, there was no observable internalization into the gut epithelial cells. Similar to carbon nanotubes and CuO NPs, our findings indicate that in daphnids the in vivo retention of Au NPs does not necessarily result in their internalization",
    author = "Khan, {Farhan R.} and Kennaway, {Gabrielle M.} and Marie-Noelle Croteau and Agnieszka Dybowska and Brian Smith and Nogueira, {Antonio J. A.} and Rainbow, {Philip S} and Samuel Luoma and Eugenia Valsami-Jones",
    year = "2014",
    doi = "10.1016/j.chemosphere.2013.12.051",
    language = "English",
    volume = "100",
    pages = "97--104",
    journal = "Chemosphere",
    issn = "0045-6535",
    publisher = "Pergamon Press",

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    Khan, FR, Kennaway, GM, Croteau, M-N, Dybowska, A, Smith, B, Nogueira, AJA, Rainbow, PS, Luoma, S & Valsami-Jones, E 2014, 'In vivo retention of ingested Au NPs by Daphnia magna: No evidence for trans-epithelial alimentary uptake', Chemosphere, bind 100, s. 97-104. https://doi.org/10.1016/j.chemosphere.2013.12.051

    In vivo retention of ingested Au NPs by Daphnia magna : No evidence for trans-epithelial alimentary uptake. / Khan, Farhan R.; Kennaway, Gabrielle M. ; Croteau, Marie-Noelle ; Dybowska, Agnieszka; Smith, Brian; Nogueira, Antonio J. A. ; Rainbow, Philip S; Luoma, Samuel; Valsami-Jones, Eugenia.

    I: Chemosphere, Bind 100, 2014, s. 97-104.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - In vivo retention of ingested Au NPs by Daphnia magna

    T2 - No evidence for trans-epithelial alimentary uptake

    AU - Khan, Farhan R.

    AU - Kennaway, Gabrielle M.

    AU - Croteau, Marie-Noelle

    AU - Dybowska, Agnieszka

    AU - Smith, Brian

    AU - Nogueira, Antonio J. A.

    AU - Rainbow, Philip S

    AU - Luoma, Samuel

    AU - Valsami-Jones, Eugenia

    PY - 2014

    Y1 - 2014

    N2 - In vivo studies with Daphnia magna remain inconclusive as to whether engineered nanoparticles (NPs) are internalized into tissues after ingestion. Here we used a three-pronged approach to study the in vivo retention and efflux kinetics of 20 nm citrate stabilized Au NPs ingested by this key aquatic species. Daphnids were exposed to suspended particles (600 μg L−1) for 5 h after which they were depurated for 24 h in clean water containing algae. Light microscopy was used to follow the passage of Au NPs through the gastrointestinal tract, Au body burdens were determined by ICP-MS (inductively coupled plasma mass spectrometry), and transmission electron microscopy (TEM) was used to examine the presence and distribution of Au NPs in tissues. Results revealed that the elimination of Au NPs was bi-phasic. The fast elimination phase lasted <1 h and the rate constant at which Au (of Au NPs) was eliminated was 1.12 ± 0.34 h−1 (±SE) which accounted for ∼75% of the ingested Au. The remaining ∼25% of the ingested Au NPs was eliminated at a 100-fold slower rate. TEM analysis revealed that Au NPs in the midgut were in close proximity to the peritrophic membrane after 1 and 24 h of depuration. There were no observations of Au NP uptake at the microvilli. Thus, although Au NPs were retained in the gut lumen, there was no observable internalization into the gut epithelial cells. Similar to carbon nanotubes and CuO NPs, our findings indicate that in daphnids the in vivo retention of Au NPs does not necessarily result in their internalization

    AB - In vivo studies with Daphnia magna remain inconclusive as to whether engineered nanoparticles (NPs) are internalized into tissues after ingestion. Here we used a three-pronged approach to study the in vivo retention and efflux kinetics of 20 nm citrate stabilized Au NPs ingested by this key aquatic species. Daphnids were exposed to suspended particles (600 μg L−1) for 5 h after which they were depurated for 24 h in clean water containing algae. Light microscopy was used to follow the passage of Au NPs through the gastrointestinal tract, Au body burdens were determined by ICP-MS (inductively coupled plasma mass spectrometry), and transmission electron microscopy (TEM) was used to examine the presence and distribution of Au NPs in tissues. Results revealed that the elimination of Au NPs was bi-phasic. The fast elimination phase lasted <1 h and the rate constant at which Au (of Au NPs) was eliminated was 1.12 ± 0.34 h−1 (±SE) which accounted for ∼75% of the ingested Au. The remaining ∼25% of the ingested Au NPs was eliminated at a 100-fold slower rate. TEM analysis revealed that Au NPs in the midgut were in close proximity to the peritrophic membrane after 1 and 24 h of depuration. There were no observations of Au NP uptake at the microvilli. Thus, although Au NPs were retained in the gut lumen, there was no observable internalization into the gut epithelial cells. Similar to carbon nanotubes and CuO NPs, our findings indicate that in daphnids the in vivo retention of Au NPs does not necessarily result in their internalization

    U2 - 10.1016/j.chemosphere.2013.12.051

    DO - 10.1016/j.chemosphere.2013.12.051

    M3 - Journal article

    VL - 100

    SP - 97

    EP - 104

    JO - Chemosphere

    JF - Chemosphere

    SN - 0045-6535

    ER -