In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs

J F Young, H C Bertram, P K Theil, A-G D Petersen, K A Poulsen, M Rasmussen, Anders Malmendal, N C Nielsen, M Vestergaard, N Oksbjerg

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Duroc and Landrace pigs as well as primary myotubes from these breeds were used to investigate mechanisms behind differences in their response to creatine monohydrate (CMH). Pigs were supplemented with 0, 12.5, 25 or 50g CMH/d for 5 days (n=10 per treatment and breed). Plasma levels of creatine increased dose-dependently in both breeds, while muscle-creatine phosphate content increased only in the Duroc pigs. (1)H NMR metabolic profiling showed a tendency towards clustering according to CMH supplementation only among Duroc pigs, revealing a stronger response compared to Landrace pigs. The abundance of insulin-like growth factor I and myostatin mRNA was decreased by CMH supplementation while that of type 1 IGF-receptor and creatine transporter was unaffected. Protein synthesis, increased in the myotubes from both breeds, indicating protein accretion, but no effect was observed on the mRNA abundance of IGF-I, type 1 IGF-receptor, myostatin or the creatine transporter in myotubes.

OriginalsprogEngelsk
TidsskriftMeat Science
Vol/bind76
Udgave nummer2
Sider (fra-til)342-51
Antal sider10
ISSN0309-1740
DOI
StatusUdgivet - jun. 2007
Udgivet eksterntJa

Citer dette

Young, J. F., Bertram, H. C., Theil, P. K., Petersen, A-G. D., Poulsen, K. A., Rasmussen, M., ... Oksbjerg, N. (2007). In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs. Meat Science, 76(2), 342-51. https://doi.org/10.1016/j.meatsci.2006.11.015
Young, J F ; Bertram, H C ; Theil, P K ; Petersen, A-G D ; Poulsen, K A ; Rasmussen, M ; Malmendal, Anders ; Nielsen, N C ; Vestergaard, M ; Oksbjerg, N. / In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs. I: Meat Science. 2007 ; Bind 76, Nr. 2. s. 342-51.
@article{7832ef4c0e1b495db50155686e05031e,
title = "In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs",
abstract = "Duroc and Landrace pigs as well as primary myotubes from these breeds were used to investigate mechanisms behind differences in their response to creatine monohydrate (CMH). Pigs were supplemented with 0, 12.5, 25 or 50g CMH/d for 5 days (n=10 per treatment and breed). Plasma levels of creatine increased dose-dependently in both breeds, while muscle-creatine phosphate content increased only in the Duroc pigs. (1)H NMR metabolic profiling showed a tendency towards clustering according to CMH supplementation only among Duroc pigs, revealing a stronger response compared to Landrace pigs. The abundance of insulin-like growth factor I and myostatin mRNA was decreased by CMH supplementation while that of type 1 IGF-receptor and creatine transporter was unaffected. Protein synthesis, increased in the myotubes from both breeds, indicating protein accretion, but no effect was observed on the mRNA abundance of IGF-I, type 1 IGF-receptor, myostatin or the creatine transporter in myotubes.",
author = "Young, {J F} and Bertram, {H C} and Theil, {P K} and Petersen, {A-G D} and Poulsen, {K A} and M Rasmussen and Anders Malmendal and Nielsen, {N C} and M Vestergaard and N Oksbjerg",
year = "2007",
month = "6",
doi = "10.1016/j.meatsci.2006.11.015",
language = "English",
volume = "76",
pages = "342--51",
journal = "Meat Science",
issn = "0309-1740",
publisher = "Elsevier BV",
number = "2",

}

Young, JF, Bertram, HC, Theil, PK, Petersen, A-GD, Poulsen, KA, Rasmussen, M, Malmendal, A, Nielsen, NC, Vestergaard, M & Oksbjerg, N 2007, 'In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs', Meat Science, bind 76, nr. 2, s. 342-51. https://doi.org/10.1016/j.meatsci.2006.11.015

In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs. / Young, J F; Bertram, H C; Theil, P K; Petersen, A-G D; Poulsen, K A; Rasmussen, M; Malmendal, Anders; Nielsen, N C; Vestergaard, M; Oksbjerg, N.

I: Meat Science, Bind 76, Nr. 2, 06.2007, s. 342-51.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs

AU - Young, J F

AU - Bertram, H C

AU - Theil, P K

AU - Petersen, A-G D

AU - Poulsen, K A

AU - Rasmussen, M

AU - Malmendal, Anders

AU - Nielsen, N C

AU - Vestergaard, M

AU - Oksbjerg, N

PY - 2007/6

Y1 - 2007/6

N2 - Duroc and Landrace pigs as well as primary myotubes from these breeds were used to investigate mechanisms behind differences in their response to creatine monohydrate (CMH). Pigs were supplemented with 0, 12.5, 25 or 50g CMH/d for 5 days (n=10 per treatment and breed). Plasma levels of creatine increased dose-dependently in both breeds, while muscle-creatine phosphate content increased only in the Duroc pigs. (1)H NMR metabolic profiling showed a tendency towards clustering according to CMH supplementation only among Duroc pigs, revealing a stronger response compared to Landrace pigs. The abundance of insulin-like growth factor I and myostatin mRNA was decreased by CMH supplementation while that of type 1 IGF-receptor and creatine transporter was unaffected. Protein synthesis, increased in the myotubes from both breeds, indicating protein accretion, but no effect was observed on the mRNA abundance of IGF-I, type 1 IGF-receptor, myostatin or the creatine transporter in myotubes.

AB - Duroc and Landrace pigs as well as primary myotubes from these breeds were used to investigate mechanisms behind differences in their response to creatine monohydrate (CMH). Pigs were supplemented with 0, 12.5, 25 or 50g CMH/d for 5 days (n=10 per treatment and breed). Plasma levels of creatine increased dose-dependently in both breeds, while muscle-creatine phosphate content increased only in the Duroc pigs. (1)H NMR metabolic profiling showed a tendency towards clustering according to CMH supplementation only among Duroc pigs, revealing a stronger response compared to Landrace pigs. The abundance of insulin-like growth factor I and myostatin mRNA was decreased by CMH supplementation while that of type 1 IGF-receptor and creatine transporter was unaffected. Protein synthesis, increased in the myotubes from both breeds, indicating protein accretion, but no effect was observed on the mRNA abundance of IGF-I, type 1 IGF-receptor, myostatin or the creatine transporter in myotubes.

U2 - 10.1016/j.meatsci.2006.11.015

DO - 10.1016/j.meatsci.2006.11.015

M3 - Journal article

VL - 76

SP - 342

EP - 351

JO - Meat Science

JF - Meat Science

SN - 0309-1740

IS - 2

ER -

Young JF, Bertram HC, Theil PK, Petersen A-GD, Poulsen KA, Rasmussen M et al. In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs. Meat Science. 2007 jun;76(2):342-51. https://doi.org/10.1016/j.meatsci.2006.11.015