In silico assessment of virulence factors in strains of Streptococcus oralis and Streptococcus mitis isolated from patients with Infective Endocarditis

Louise Hesselbjerg Rasmussen, Katrine Højholt, Rimtas Dargis, Jens Jørgen Christensen, Ole Skovgaard, Ulrik Justesen, Flemming Rosenvinge, Claus Moser, Oksana Lukjancenko, Simon Rasmussen, Xiaohui Chen Nielsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Purpose. Streptococcus oralis and Streptococcus mitis belong to the Mitis group, which are mostly commensals in the human oral cavity. Even though S. oralis and S. mitis are oral commensals, they can be opportunistic pathogens causing infective endocarditis. A recent taxonomic re-evaluation of the Mitis group has embedded the species Streptococcus tigurinus and Streptococcus dentisani into the species S. oralis as subspecies. In this study, the distribution of virulence factors that contribute to bacterial immune evasion, colonization and adhesion was assessed in clinical strains of S. oralis (subsp. oralis, subsp. tigurinus and subsp. dentisani) and S. mitis.

Methodology. Forty clinical S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) and S. mitis genomes were annotated with the pipeline PanFunPro and aligned against the VFDB database for assessment of virulence factors.

Results/Key findings. Three homologues of pavA, psaA and lmb, encoding adhesion proteins, were present in all strains. Seven homologues of nanA, nanB, ply, lytA, lytB, lytC and iga, of importance regarding survival in blood and modulation of the human immune system, were variously present in the genomes. Few S. oralis subspecies specific differences were observed. iga homologues were identified in S. oralis subsp. oralis, whereas lytA homologues were identified in S. oralis subsp. oralis and subsp. tigurinus.

Conclusion. Differences in the presence of virulence factors among the three S. oralis subspecies were observed. The virulence gene profiles of the 40 S. mitis and S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) contribute with important new knowledge regarding these species and new subspecies.
OriginalsprogEngelsk
TidsskriftJournal of Medical Microbiology
Vol/bind66
Sider (fra-til)1316-1323
Antal sider8
ISSN0022-2615
DOI
StatusUdgivet - 2017

Citer dette

Rasmussen, Louise Hesselbjerg ; Højholt, Katrine ; Dargis, Rimtas ; Christensen, Jens Jørgen ; Skovgaard, Ole ; Justesen, Ulrik ; Rosenvinge, Flemming ; Moser, Claus ; Lukjancenko, Oksana ; Rasmussen, Simon ; Nielsen, Xiaohui Chen . / In silico assessment of virulence factors in strains of Streptococcus oralis and Streptococcus mitis isolated from patients with Infective Endocarditis. I: Journal of Medical Microbiology. 2017 ; Bind 66. s. 1316-1323.
@article{c0d4bf2ce6c742fcb32e02b6fed7ac61,
title = "In silico assessment of virulence factors in strains of Streptococcus oralis and Streptococcus mitis isolated from patients with Infective Endocarditis",
abstract = "Purpose. Streptococcus oralis and Streptococcus mitis belong to the Mitis group, which are mostly commensals in the human oral cavity. Even though S. oralis and S. mitis are oral commensals, they can be opportunistic pathogens causing infective endocarditis. A recent taxonomic re-evaluation of the Mitis group has embedded the species Streptococcus tigurinus and Streptococcus dentisani into the species S. oralis as subspecies. In this study, the distribution of virulence factors that contribute to bacterial immune evasion, colonization and adhesion was assessed in clinical strains of S. oralis (subsp. oralis, subsp. tigurinus and subsp. dentisani) and S. mitis.Methodology. Forty clinical S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) and S. mitis genomes were annotated with the pipeline PanFunPro and aligned against the VFDB database for assessment of virulence factors.Results/Key findings. Three homologues of pavA, psaA and lmb, encoding adhesion proteins, were present in all strains. Seven homologues of nanA, nanB, ply, lytA, lytB, lytC and iga, of importance regarding survival in blood and modulation of the human immune system, were variously present in the genomes. Few S. oralis subspecies specific differences were observed. iga homologues were identified in S. oralis subsp. oralis, whereas lytA homologues were identified in S. oralis subsp. oralis and subsp. tigurinus.Conclusion. Differences in the presence of virulence factors among the three S. oralis subspecies were observed. The virulence gene profiles of the 40 S. mitis and S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) contribute with important new knowledge regarding these species and new subspecies.",
keywords = "virulence factors, Streptococcus oralis, Streptococcus mitis, infective endocarditis, comparative genomics",
author = "Rasmussen, {Louise Hesselbjerg} and Katrine H{\o}jholt and Rimtas Dargis and Christensen, {Jens J{\o}rgen} and Ole Skovgaard and Ulrik Justesen and Flemming Rosenvinge and Claus Moser and Oksana Lukjancenko and Simon Rasmussen and Nielsen, {Xiaohui Chen}",
year = "2017",
doi = "10.1099/jmm.0.000573",
language = "English",
volume = "66",
pages = "1316--1323",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "TheMicrobiology Society",

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Rasmussen, LH, Højholt, K, Dargis, R, Christensen, JJ, Skovgaard, O, Justesen, U, Rosenvinge, F, Moser, C, Lukjancenko, O, Rasmussen, S & Nielsen, XC 2017, 'In silico assessment of virulence factors in strains of Streptococcus oralis and Streptococcus mitis isolated from patients with Infective Endocarditis', Journal of Medical Microbiology, bind 66, s. 1316-1323. https://doi.org/10.1099/jmm.0.000573

In silico assessment of virulence factors in strains of Streptococcus oralis and Streptococcus mitis isolated from patients with Infective Endocarditis. / Rasmussen, Louise Hesselbjerg; Højholt, Katrine ; Dargis, Rimtas; Christensen, Jens Jørgen; Skovgaard, Ole; Justesen, Ulrik ; Rosenvinge, Flemming ; Moser, Claus; Lukjancenko, Oksana; Rasmussen, Simon; Nielsen, Xiaohui Chen .

I: Journal of Medical Microbiology, Bind 66, 2017, s. 1316-1323.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - In silico assessment of virulence factors in strains of Streptococcus oralis and Streptococcus mitis isolated from patients with Infective Endocarditis

AU - Rasmussen, Louise Hesselbjerg

AU - Højholt, Katrine

AU - Dargis, Rimtas

AU - Christensen, Jens Jørgen

AU - Skovgaard, Ole

AU - Justesen, Ulrik

AU - Rosenvinge, Flemming

AU - Moser, Claus

AU - Lukjancenko, Oksana

AU - Rasmussen, Simon

AU - Nielsen, Xiaohui Chen

PY - 2017

Y1 - 2017

N2 - Purpose. Streptococcus oralis and Streptococcus mitis belong to the Mitis group, which are mostly commensals in the human oral cavity. Even though S. oralis and S. mitis are oral commensals, they can be opportunistic pathogens causing infective endocarditis. A recent taxonomic re-evaluation of the Mitis group has embedded the species Streptococcus tigurinus and Streptococcus dentisani into the species S. oralis as subspecies. In this study, the distribution of virulence factors that contribute to bacterial immune evasion, colonization and adhesion was assessed in clinical strains of S. oralis (subsp. oralis, subsp. tigurinus and subsp. dentisani) and S. mitis.Methodology. Forty clinical S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) and S. mitis genomes were annotated with the pipeline PanFunPro and aligned against the VFDB database for assessment of virulence factors.Results/Key findings. Three homologues of pavA, psaA and lmb, encoding adhesion proteins, were present in all strains. Seven homologues of nanA, nanB, ply, lytA, lytB, lytC and iga, of importance regarding survival in blood and modulation of the human immune system, were variously present in the genomes. Few S. oralis subspecies specific differences were observed. iga homologues were identified in S. oralis subsp. oralis, whereas lytA homologues were identified in S. oralis subsp. oralis and subsp. tigurinus.Conclusion. Differences in the presence of virulence factors among the three S. oralis subspecies were observed. The virulence gene profiles of the 40 S. mitis and S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) contribute with important new knowledge regarding these species and new subspecies.

AB - Purpose. Streptococcus oralis and Streptococcus mitis belong to the Mitis group, which are mostly commensals in the human oral cavity. Even though S. oralis and S. mitis are oral commensals, they can be opportunistic pathogens causing infective endocarditis. A recent taxonomic re-evaluation of the Mitis group has embedded the species Streptococcus tigurinus and Streptococcus dentisani into the species S. oralis as subspecies. In this study, the distribution of virulence factors that contribute to bacterial immune evasion, colonization and adhesion was assessed in clinical strains of S. oralis (subsp. oralis, subsp. tigurinus and subsp. dentisani) and S. mitis.Methodology. Forty clinical S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) and S. mitis genomes were annotated with the pipeline PanFunPro and aligned against the VFDB database for assessment of virulence factors.Results/Key findings. Three homologues of pavA, psaA and lmb, encoding adhesion proteins, were present in all strains. Seven homologues of nanA, nanB, ply, lytA, lytB, lytC and iga, of importance regarding survival in blood and modulation of the human immune system, were variously present in the genomes. Few S. oralis subspecies specific differences were observed. iga homologues were identified in S. oralis subsp. oralis, whereas lytA homologues were identified in S. oralis subsp. oralis and subsp. tigurinus.Conclusion. Differences in the presence of virulence factors among the three S. oralis subspecies were observed. The virulence gene profiles of the 40 S. mitis and S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) contribute with important new knowledge regarding these species and new subspecies.

KW - virulence factors

KW - Streptococcus oralis

KW - Streptococcus mitis

KW - infective endocarditis

KW - comparative genomics

U2 - 10.1099/jmm.0.000573

DO - 10.1099/jmm.0.000573

M3 - Journal article

VL - 66

SP - 1316

EP - 1323

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

ER -