In-hospital metabolite changes in infective endocarditis

a longitudinal 1H NMR-based study

Christine Falk Klein, Sarah Louise Kjølhede Holle, Malene Højgaard Andersen, Anders Pedersen, Henning Bundgaard, Kasper Karmark Iversen, Anders Malmendal

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Treatment of infective endocarditis (IE) is a 4-6-week provided course of intravenously administered antibiotics. The aim of this study was to investigate how serum metabolites as measured by proton nuclear magnetic resonance (1H NMR) spectroscopy are changing over time during the active phase of IE, and to see whether these metabolite changes might be used to monitor recovery in these patients. Patients hospitalized with first-time IE at Herlev Hospital, Denmark, from September 2015 to June 2017 were included. Longitudinal blood sampling was performed and serum was analyzed using 1H NMR. Orthogonal projection to latent structures discriminant analysis (OPLS-DA) was used to separate sample groups and analyze differences in metabolite profiles. Thirteen patients were included in the study (77% men, median age 62 years (IQR 53-77)). All patients were cured during the hospitalization without any relapse during 6 months of follow-up. We analyzed 61 serum samples (median 5 samples, range 2-8 per person) drawn in the treatment period after IE diagnosis. The main changes during the in-hospital period were decreased levels of glucose, mannose, leucine, isoleucine, phenylalanine, tyrosine, and signals from polyols and N-acetylated protein. The metabolomic changes could in contrast to the routinely used parameters CRP and leucocyte levels distinguish between the early and late stages of disease treatment. We present the first longitudinal study of 1H NMR metabolomics in patients with infective endocarditis. The metabolomic changes show a promising strength compared to routinely used clinical parameters.
OriginalsprogEngelsk
TidsskriftEuropean Journal of Clinical Microbiology & Infectious Diseases
Vol/bind2019
Udgave nummer38
Sider (fra-til)1553-1560
ISSN0934-9723
DOI
StatusUdgivet - 2019
Udgivet eksterntJa

Emneord

  • Antibiotic treatment
  • Infective endocarditis
  • Longitudinal study
  • Metabolomics
  • NMR

Citer dette

Klein, Christine Falk ; Holle, Sarah Louise Kjølhede ; Andersen, Malene Højgaard ; Pedersen, Anders ; Bundgaard, Henning ; Iversen, Kasper Karmark ; Malmendal, Anders. / In-hospital metabolite changes in infective endocarditis : a longitudinal 1H NMR-based study. I: European Journal of Clinical Microbiology & Infectious Diseases. 2019 ; Bind 2019, Nr. 38. s. 1553-1560.
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abstract = "Treatment of infective endocarditis (IE) is a 4-6-week provided course of intravenously administered antibiotics. The aim of this study was to investigate how serum metabolites as measured by proton nuclear magnetic resonance (1H NMR) spectroscopy are changing over time during the active phase of IE, and to see whether these metabolite changes might be used to monitor recovery in these patients. Patients hospitalized with first-time IE at Herlev Hospital, Denmark, from September 2015 to June 2017 were included. Longitudinal blood sampling was performed and serum was analyzed using 1H NMR. Orthogonal projection to latent structures discriminant analysis (OPLS-DA) was used to separate sample groups and analyze differences in metabolite profiles. Thirteen patients were included in the study (77{\%} men, median age 62 years (IQR 53-77)). All patients were cured during the hospitalization without any relapse during 6 months of follow-up. We analyzed 61 serum samples (median 5 samples, range 2-8 per person) drawn in the treatment period after IE diagnosis. The main changes during the in-hospital period were decreased levels of glucose, mannose, leucine, isoleucine, phenylalanine, tyrosine, and signals from polyols and N-acetylated protein. The metabolomic changes could in contrast to the routinely used parameters CRP and leucocyte levels distinguish between the early and late stages of disease treatment. We present the first longitudinal study of 1H NMR metabolomics in patients with infective endocarditis. The metabolomic changes show a promising strength compared to routinely used clinical parameters.",
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author = "Klein, {Christine Falk} and Holle, {Sarah Louise Kj{\o}lhede} and Andersen, {Malene H{\o}jgaard} and Anders Pedersen and Henning Bundgaard and Iversen, {Kasper Karmark} and Anders Malmendal",
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In-hospital metabolite changes in infective endocarditis : a longitudinal 1H NMR-based study. / Klein, Christine Falk; Holle, Sarah Louise Kjølhede; Andersen, Malene Højgaard; Pedersen, Anders; Bundgaard, Henning; Iversen, Kasper Karmark; Malmendal, Anders.

I: European Journal of Clinical Microbiology & Infectious Diseases, Bind 2019, Nr. 38, 2019, s. 1553-1560.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - In-hospital metabolite changes in infective endocarditis

T2 - a longitudinal 1H NMR-based study

AU - Klein, Christine Falk

AU - Holle, Sarah Louise Kjølhede

AU - Andersen, Malene Højgaard

AU - Pedersen, Anders

AU - Bundgaard, Henning

AU - Iversen, Kasper Karmark

AU - Malmendal, Anders

PY - 2019

Y1 - 2019

N2 - Treatment of infective endocarditis (IE) is a 4-6-week provided course of intravenously administered antibiotics. The aim of this study was to investigate how serum metabolites as measured by proton nuclear magnetic resonance (1H NMR) spectroscopy are changing over time during the active phase of IE, and to see whether these metabolite changes might be used to monitor recovery in these patients. Patients hospitalized with first-time IE at Herlev Hospital, Denmark, from September 2015 to June 2017 were included. Longitudinal blood sampling was performed and serum was analyzed using 1H NMR. Orthogonal projection to latent structures discriminant analysis (OPLS-DA) was used to separate sample groups and analyze differences in metabolite profiles. Thirteen patients were included in the study (77% men, median age 62 years (IQR 53-77)). All patients were cured during the hospitalization without any relapse during 6 months of follow-up. We analyzed 61 serum samples (median 5 samples, range 2-8 per person) drawn in the treatment period after IE diagnosis. The main changes during the in-hospital period were decreased levels of glucose, mannose, leucine, isoleucine, phenylalanine, tyrosine, and signals from polyols and N-acetylated protein. The metabolomic changes could in contrast to the routinely used parameters CRP and leucocyte levels distinguish between the early and late stages of disease treatment. We present the first longitudinal study of 1H NMR metabolomics in patients with infective endocarditis. The metabolomic changes show a promising strength compared to routinely used clinical parameters.

AB - Treatment of infective endocarditis (IE) is a 4-6-week provided course of intravenously administered antibiotics. The aim of this study was to investigate how serum metabolites as measured by proton nuclear magnetic resonance (1H NMR) spectroscopy are changing over time during the active phase of IE, and to see whether these metabolite changes might be used to monitor recovery in these patients. Patients hospitalized with first-time IE at Herlev Hospital, Denmark, from September 2015 to June 2017 were included. Longitudinal blood sampling was performed and serum was analyzed using 1H NMR. Orthogonal projection to latent structures discriminant analysis (OPLS-DA) was used to separate sample groups and analyze differences in metabolite profiles. Thirteen patients were included in the study (77% men, median age 62 years (IQR 53-77)). All patients were cured during the hospitalization without any relapse during 6 months of follow-up. We analyzed 61 serum samples (median 5 samples, range 2-8 per person) drawn in the treatment period after IE diagnosis. The main changes during the in-hospital period were decreased levels of glucose, mannose, leucine, isoleucine, phenylalanine, tyrosine, and signals from polyols and N-acetylated protein. The metabolomic changes could in contrast to the routinely used parameters CRP and leucocyte levels distinguish between the early and late stages of disease treatment. We present the first longitudinal study of 1H NMR metabolomics in patients with infective endocarditis. The metabolomic changes show a promising strength compared to routinely used clinical parameters.

KW - Antibiotic treatment

KW - Infective endocarditis

KW - Longitudinal study

KW - Metabolomics

KW - NMR

U2 - 10.1007/s10096-019-03586-z

DO - 10.1007/s10096-019-03586-z

M3 - Journal article

VL - 2019

SP - 1553

EP - 1560

JO - European Journal of Clinical Microbiology & Infectious Diseases

JF - European Journal of Clinical Microbiology & Infectious Diseases

SN - 0934-9723

IS - 38

ER -