Human leukocyte antigen-G in the male reproductive system and in seminal plasma

Margit Horup Larsen, Michael Bzorek, Malene B. Pass, Lise Grupe Larsen, Mette Weidinger Nielsen, Signe Goul Svendsen, Anette Lindhard, Thomas V. F. Hviis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    One of the non-classical human leukocyte antigen (HLA) class Ib proteins, HLA-G, is believed to exert important immunoregulatory functions, especially during pregnancy. The presence of HLA protein in paternal seminal fluid has been suggested to have an influence on the risk of developing pre-eclampsia. We have investigated whether HLA-G protein is present in human seminal plasma and in different tissue samples of the male reproductive system.Western blot technique and a soluble HLA-G (sHLA-G) assay were used to detect sHLA-G in human
    seminal plasma samples. Immunohistochemical staining was performed on paraffin-embedded tissue samples. We detected sHLA-G protein in
    seminal plasma, and HLA-G expression in normal testis and in epididymal tissue of the male reproductive system but not in the seminal vesicle.
    Furthermore, the results indicated a weak expression of HLA–G in hyperplastic prostatic tissue. In summary, several of the findings reported in
    this study suggest an immunoregulatory role of HLA-G in the male reproductive system and in seminal plasma.
    OriginalsprogEngelsk
    TidsskriftMolecular Human Reproduction
    Vol/bind17
    Udgave nummer12
    Sider (fra-til)727–738
    ISSN1360-9947
    DOI
    StatusUdgivet - 2011

    Citer dette

    Horup Larsen, M., Bzorek, M., Pass, M. B., Grupe Larsen, L., Weidinger Nielsen, M., Goul Svendsen, S., ... Hviis, T. V. F. (2011). Human leukocyte antigen-G in the male reproductive system and in seminal plasma. Molecular Human Reproduction, 17(12), 727–738. https://doi.org/10.1093/molehr/gar052
    Horup Larsen, Margit ; Bzorek, Michael ; Pass, Malene B. ; Grupe Larsen, Lise ; Weidinger Nielsen, Mette ; Goul Svendsen, Signe ; Lindhard, Anette ; Hviis, Thomas V. F. / Human leukocyte antigen-G in the male reproductive system and in seminal plasma. I: Molecular Human Reproduction. 2011 ; Bind 17, Nr. 12. s. 727–738.
    @article{b51f0ef62d5c4fafa93ba5ec6dcb4097,
    title = "Human leukocyte antigen-G in the male reproductive system and in seminal plasma",
    abstract = "One of the non-classical human leukocyte antigen (HLA) class Ib proteins, HLA-G, is believed to exert important immunoregulatory functions, especially during pregnancy. The presence of HLA protein in paternal seminal fluid has been suggested to have an influence on the risk of developing pre-eclampsia. We have investigated whether HLA-G protein is present in human seminal plasma and in different tissue samples of the male reproductive system.Western blot technique and a soluble HLA-G (sHLA-G) assay were used to detect sHLA-G in human seminal plasma samples. Immunohistochemical staining was performed on paraffin-embedded tissue samples. We detected sHLA-G protein in seminal plasma, and HLA-G expression in normal testis and in epididymal tissue of the male reproductive system but not in the seminal vesicle. Furthermore, the results indicated a weak expression of HLA–G in hyperplastic prostatic tissue. In summary, several of the findings reported in this study suggest an immunoregulatory role of HLA-G in the male reproductive system and in seminal plasma.",
    keywords = "MHC, HLA_G, reproduction, seminal plasma, male reproductive system",
    author = "{Horup Larsen}, Margit and Michael Bzorek and Pass, {Malene B.} and {Grupe Larsen}, Lise and {Weidinger Nielsen}, Mette and {Goul Svendsen}, Signe and Anette Lindhard and Hviis, {Thomas V. F.}",
    year = "2011",
    doi = "10.1093/molehr/gar052",
    language = "English",
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    pages = "727–738",
    journal = "Molecular Human Reproduction",
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    Horup Larsen, M, Bzorek, M, Pass, MB, Grupe Larsen, L, Weidinger Nielsen, M, Goul Svendsen, S, Lindhard, A & Hviis, TVF 2011, 'Human leukocyte antigen-G in the male reproductive system and in seminal plasma', Molecular Human Reproduction, bind 17, nr. 12, s. 727–738. https://doi.org/10.1093/molehr/gar052

    Human leukocyte antigen-G in the male reproductive system and in seminal plasma. / Horup Larsen, Margit; Bzorek, Michael; Pass, Malene B.; Grupe Larsen, Lise; Weidinger Nielsen, Mette; Goul Svendsen, Signe; Lindhard, Anette; Hviis, Thomas V. F.

    I: Molecular Human Reproduction, Bind 17, Nr. 12, 2011, s. 727–738.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Human leukocyte antigen-G in the male reproductive system and in seminal plasma

    AU - Horup Larsen, Margit

    AU - Bzorek, Michael

    AU - Pass, Malene B.

    AU - Grupe Larsen, Lise

    AU - Weidinger Nielsen, Mette

    AU - Goul Svendsen, Signe

    AU - Lindhard, Anette

    AU - Hviis, Thomas V. F.

    PY - 2011

    Y1 - 2011

    N2 - One of the non-classical human leukocyte antigen (HLA) class Ib proteins, HLA-G, is believed to exert important immunoregulatory functions, especially during pregnancy. The presence of HLA protein in paternal seminal fluid has been suggested to have an influence on the risk of developing pre-eclampsia. We have investigated whether HLA-G protein is present in human seminal plasma and in different tissue samples of the male reproductive system.Western blot technique and a soluble HLA-G (sHLA-G) assay were used to detect sHLA-G in human seminal plasma samples. Immunohistochemical staining was performed on paraffin-embedded tissue samples. We detected sHLA-G protein in seminal plasma, and HLA-G expression in normal testis and in epididymal tissue of the male reproductive system but not in the seminal vesicle. Furthermore, the results indicated a weak expression of HLA–G in hyperplastic prostatic tissue. In summary, several of the findings reported in this study suggest an immunoregulatory role of HLA-G in the male reproductive system and in seminal plasma.

    AB - One of the non-classical human leukocyte antigen (HLA) class Ib proteins, HLA-G, is believed to exert important immunoregulatory functions, especially during pregnancy. The presence of HLA protein in paternal seminal fluid has been suggested to have an influence on the risk of developing pre-eclampsia. We have investigated whether HLA-G protein is present in human seminal plasma and in different tissue samples of the male reproductive system.Western blot technique and a soluble HLA-G (sHLA-G) assay were used to detect sHLA-G in human seminal plasma samples. Immunohistochemical staining was performed on paraffin-embedded tissue samples. We detected sHLA-G protein in seminal plasma, and HLA-G expression in normal testis and in epididymal tissue of the male reproductive system but not in the seminal vesicle. Furthermore, the results indicated a weak expression of HLA–G in hyperplastic prostatic tissue. In summary, several of the findings reported in this study suggest an immunoregulatory role of HLA-G in the male reproductive system and in seminal plasma.

    KW - MHC

    KW - HLA_G

    KW - reproduction

    KW - seminal plasma

    KW - male reproductive system

    U2 - 10.1093/molehr/gar052

    DO - 10.1093/molehr/gar052

    M3 - Journal article

    VL - 17

    SP - 727

    EP - 738

    JO - Molecular Human Reproduction

    JF - Molecular Human Reproduction

    SN - 1360-9947

    IS - 12

    ER -

    Horup Larsen M, Bzorek M, Pass MB, Grupe Larsen L, Weidinger Nielsen M, Goul Svendsen S et al. Human leukocyte antigen-G in the male reproductive system and in seminal plasma. Molecular Human Reproduction. 2011;17(12):727–738. https://doi.org/10.1093/molehr/gar052