Hsp70 expression and metabolite composition in response to short-term thermal changes in Folsomia candida (Collembola)

Dorthe Waagner, Lars-Henrik Heckmann, Anders Malmendal, Niels Chr Nielsen, Martin Holmstrup, Mark Bayley

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

In the present study the joint transcriptomic and metabolomic responses in Folsomia candida (Collembola) to temperature changes on a short-term scale were studied. Change in heat tolerance was examined as survival after a 35 degrees C heat shock (2h) in the course of either a fluctuating temperature regime (8 to 32 degrees C; pre-treated) or a constant temperature (20 degrees C; control) over a period of 24h. Exposure to a temperature increase from 20 to 32 degrees C (2.4 degrees C min(-)(1)) induced a significantly increased heat tolerance which continued throughout the experiment. Expression of the gene encoding heat shock protein Hsp70 was assessed at the mRNA level using real time quantitative polymerase chain reaction (QPCR). Hsp70 was rapidly induced and significantly increased by the temperature increase. The relative concentrations of low molecular weight metabolites were analysed in F. candida using nuclear magnetic resonance spectroscopy ((1)H NMR). A significant metabolomic divergence between pre-treated and control collembolans was evident; partly due to a significantly reduced relative concentration of five free amino acids (arginine, leucine, lysine, phenylalanine and tyrosine) in pre-treated collembolans. These results, obtained from ecological transcriptomics and metabolomics jointly generated insight on various levels into the combined responses to a changing environment.
OriginalsprogEngelsk
TidsskriftComparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
Vol/bind157
Udgave nummer2
Sider (fra-til)177-83
Antal sider7
ISSN1095-6433
DOI
StatusUdgivet - 1 okt. 2010
Udgivet eksterntJa

Citer dette

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title = "Hsp70 expression and metabolite composition in response to short-term thermal changes in Folsomia candida (Collembola)",
abstract = "In the present study the joint transcriptomic and metabolomic responses in Folsomia candida (Collembola) to temperature changes on a short-term scale were studied. Change in heat tolerance was examined as survival after a 35 degrees C heat shock (2h) in the course of either a fluctuating temperature regime (8 to 32 degrees C; pre-treated) or a constant temperature (20 degrees C; control) over a period of 24h. Exposure to a temperature increase from 20 to 32 degrees C (2.4 degrees C min(-)(1)) induced a significantly increased heat tolerance which continued throughout the experiment. Expression of the gene encoding heat shock protein Hsp70 was assessed at the mRNA level using real time quantitative polymerase chain reaction (QPCR). Hsp70 was rapidly induced and significantly increased by the temperature increase. The relative concentrations of low molecular weight metabolites were analysed in F. candida using nuclear magnetic resonance spectroscopy ((1)H NMR). A significant metabolomic divergence between pre-treated and control collembolans was evident; partly due to a significantly reduced relative concentration of five free amino acids (arginine, leucine, lysine, phenylalanine and tyrosine) in pre-treated collembolans. These results, obtained from ecological transcriptomics and metabolomics jointly generated insight on various levels into the combined responses to a changing environment.",
author = "Dorthe Waagner and Lars-Henrik Heckmann and Anders Malmendal and Nielsen, {Niels Chr} and Martin Holmstrup and Mark Bayley",
note = "2010 Elsevier Inc. All rights reserved.",
year = "2010",
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Hsp70 expression and metabolite composition in response to short-term thermal changes in Folsomia candida (Collembola). / Waagner, Dorthe; Heckmann, Lars-Henrik; Malmendal, Anders; Nielsen, Niels Chr; Holmstrup, Martin; Bayley, Mark.

I: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, Bind 157, Nr. 2, 01.10.2010, s. 177-83.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Hsp70 expression and metabolite composition in response to short-term thermal changes in Folsomia candida (Collembola)

AU - Waagner, Dorthe

AU - Heckmann, Lars-Henrik

AU - Malmendal, Anders

AU - Nielsen, Niels Chr

AU - Holmstrup, Martin

AU - Bayley, Mark

N1 - 2010 Elsevier Inc. All rights reserved.

PY - 2010/10/1

Y1 - 2010/10/1

N2 - In the present study the joint transcriptomic and metabolomic responses in Folsomia candida (Collembola) to temperature changes on a short-term scale were studied. Change in heat tolerance was examined as survival after a 35 degrees C heat shock (2h) in the course of either a fluctuating temperature regime (8 to 32 degrees C; pre-treated) or a constant temperature (20 degrees C; control) over a period of 24h. Exposure to a temperature increase from 20 to 32 degrees C (2.4 degrees C min(-)(1)) induced a significantly increased heat tolerance which continued throughout the experiment. Expression of the gene encoding heat shock protein Hsp70 was assessed at the mRNA level using real time quantitative polymerase chain reaction (QPCR). Hsp70 was rapidly induced and significantly increased by the temperature increase. The relative concentrations of low molecular weight metabolites were analysed in F. candida using nuclear magnetic resonance spectroscopy ((1)H NMR). A significant metabolomic divergence between pre-treated and control collembolans was evident; partly due to a significantly reduced relative concentration of five free amino acids (arginine, leucine, lysine, phenylalanine and tyrosine) in pre-treated collembolans. These results, obtained from ecological transcriptomics and metabolomics jointly generated insight on various levels into the combined responses to a changing environment.

AB - In the present study the joint transcriptomic and metabolomic responses in Folsomia candida (Collembola) to temperature changes on a short-term scale were studied. Change in heat tolerance was examined as survival after a 35 degrees C heat shock (2h) in the course of either a fluctuating temperature regime (8 to 32 degrees C; pre-treated) or a constant temperature (20 degrees C; control) over a period of 24h. Exposure to a temperature increase from 20 to 32 degrees C (2.4 degrees C min(-)(1)) induced a significantly increased heat tolerance which continued throughout the experiment. Expression of the gene encoding heat shock protein Hsp70 was assessed at the mRNA level using real time quantitative polymerase chain reaction (QPCR). Hsp70 was rapidly induced and significantly increased by the temperature increase. The relative concentrations of low molecular weight metabolites were analysed in F. candida using nuclear magnetic resonance spectroscopy ((1)H NMR). A significant metabolomic divergence between pre-treated and control collembolans was evident; partly due to a significantly reduced relative concentration of five free amino acids (arginine, leucine, lysine, phenylalanine and tyrosine) in pre-treated collembolans. These results, obtained from ecological transcriptomics and metabolomics jointly generated insight on various levels into the combined responses to a changing environment.

U2 - 10.1016/j.cbpa.2010.06.171

DO - 10.1016/j.cbpa.2010.06.171

M3 - Journal article

VL - 157

SP - 177

EP - 183

JO - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

JF - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

SN - 1095-6433

IS - 2

ER -