Germline mutation rates in mice following in utero exposure to diesel exhaust particles by maternal inhalation

Caitlin Ritz, Wojciech Ruminski, Karin Hougaard, Håkan Wallin, Ulla Vogel, Carole L. Yauk

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    The induction of inherited DNA sequence mutations arising in the germline (i.e., sperm or egg) of mice exposed in utero to diesel exhaust particles (DEPs) via maternal inhalation compared to unexposed controls was investigated in this study. Previous work has shown that particulate air pollutants (PAPs) from industrial environments cause DNA damage and mutations in the sperm of adult male mice. Effects on the female and male germline during critical stages of development (in utero) are unknown. In mice, previous studies have shown that expanded simple tandem repeat (ESTR) loci exhibit high rates of spontaneous mutation, making this endpoint a valuable tool for studying inherited mutation and genomic instability. In the present study, pregnant C57Bl/6 mice were exposed to 19 mg/m3 DEP from gestational day 7 through 19, alongside air exposed controls. Male and female F1 offspring were raised to maturity and mated with control CBA mice. The F2 descendents were collected and ESTR germline mutation rates were derived from full pedigrees (mother, father, offspring) of F1 male and female mice. We found no evidence for increased ESTR mutation rates in females exposed in utero to DEP relative to control females. In contrast, a statistically significant increase in the mutation frequency of male mice exposed in utero to DEP was observed (2-fold; Fisher's exact p < 0.05). Thus, maternal exposure to DEP results in increased mutation in sperm during development.

    OriginalsprogEngelsk
    TidsskriftMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
    Vol/bind712
    Udgave nummer1-2
    Sider (fra-til)55-58
    ISSN0027-5107
    DOI
    StatusUdgivet - 2011

    Citer dette

    Ritz, Caitlin ; Ruminski, Wojciech ; Hougaard, Karin ; Wallin, Håkan ; Vogel, Ulla ; Yauk, Carole L. / Germline mutation rates in mice following in utero exposure to diesel exhaust particles by maternal inhalation. I: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2011 ; Bind 712, Nr. 1-2. s. 55-58.
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    title = "Germline mutation rates in mice following in utero exposure to diesel exhaust particles by maternal inhalation",
    abstract = "The induction of inherited DNA sequence mutations arising in the germline (i.e., sperm or egg) of mice exposed in utero to diesel exhaust particles (DEPs) via maternal inhalation compared to unexposed controls was investigated in this study. Previous work has shown that particulate air pollutants (PAPs) from industrial environments cause DNA damage and mutations in the sperm of adult male mice. Effects on the female and male germline during critical stages of development (in utero) are unknown. In mice, previous studies have shown that expanded simple tandem repeat (ESTR) loci exhibit high rates of spontaneous mutation, making this endpoint a valuable tool for studying inherited mutation and genomic instability. In the present study, pregnant C57Bl/6 mice were exposed to 19 mg/m3 DEP from gestational day 7 through 19, alongside air exposed controls. Male and female F1 offspring were raised to maturity and mated with control CBA mice. The F2 descendents were collected and ESTR germline mutation rates were derived from full pedigrees (mother, father, offspring) of F1 male and female mice. We found no evidence for increased ESTR mutation rates in females exposed in utero to DEP relative to control females. In contrast, a statistically significant increase in the mutation frequency of male mice exposed in utero to DEP was observed (2-fold; Fisher's exact p < 0.05). Thus, maternal exposure to DEP results in increased mutation in sperm during development.",
    keywords = "ESTRs, DEP, Germline instability, Epigenetics",
    author = "Caitlin Ritz and Wojciech Ruminski and Karin Hougaard and H{\aa}kan Wallin and Ulla Vogel and Yauk, {Carole L.}",
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    Germline mutation rates in mice following in utero exposure to diesel exhaust particles by maternal inhalation. / Ritz, Caitlin; Ruminski, Wojciech; Hougaard, Karin ; Wallin, Håkan; Vogel, Ulla; Yauk, Carole L.

    I: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Bind 712, Nr. 1-2, 2011, s. 55-58.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Germline mutation rates in mice following in utero exposure to diesel exhaust particles by maternal inhalation

    AU - Ritz, Caitlin

    AU - Ruminski, Wojciech

    AU - Hougaard, Karin

    AU - Wallin, Håkan

    AU - Vogel, Ulla

    AU - Yauk, Carole L.

    PY - 2011

    Y1 - 2011

    N2 - The induction of inherited DNA sequence mutations arising in the germline (i.e., sperm or egg) of mice exposed in utero to diesel exhaust particles (DEPs) via maternal inhalation compared to unexposed controls was investigated in this study. Previous work has shown that particulate air pollutants (PAPs) from industrial environments cause DNA damage and mutations in the sperm of adult male mice. Effects on the female and male germline during critical stages of development (in utero) are unknown. In mice, previous studies have shown that expanded simple tandem repeat (ESTR) loci exhibit high rates of spontaneous mutation, making this endpoint a valuable tool for studying inherited mutation and genomic instability. In the present study, pregnant C57Bl/6 mice were exposed to 19 mg/m3 DEP from gestational day 7 through 19, alongside air exposed controls. Male and female F1 offspring were raised to maturity and mated with control CBA mice. The F2 descendents were collected and ESTR germline mutation rates were derived from full pedigrees (mother, father, offspring) of F1 male and female mice. We found no evidence for increased ESTR mutation rates in females exposed in utero to DEP relative to control females. In contrast, a statistically significant increase in the mutation frequency of male mice exposed in utero to DEP was observed (2-fold; Fisher's exact p < 0.05). Thus, maternal exposure to DEP results in increased mutation in sperm during development.

    AB - The induction of inherited DNA sequence mutations arising in the germline (i.e., sperm or egg) of mice exposed in utero to diesel exhaust particles (DEPs) via maternal inhalation compared to unexposed controls was investigated in this study. Previous work has shown that particulate air pollutants (PAPs) from industrial environments cause DNA damage and mutations in the sperm of adult male mice. Effects on the female and male germline during critical stages of development (in utero) are unknown. In mice, previous studies have shown that expanded simple tandem repeat (ESTR) loci exhibit high rates of spontaneous mutation, making this endpoint a valuable tool for studying inherited mutation and genomic instability. In the present study, pregnant C57Bl/6 mice were exposed to 19 mg/m3 DEP from gestational day 7 through 19, alongside air exposed controls. Male and female F1 offspring were raised to maturity and mated with control CBA mice. The F2 descendents were collected and ESTR germline mutation rates were derived from full pedigrees (mother, father, offspring) of F1 male and female mice. We found no evidence for increased ESTR mutation rates in females exposed in utero to DEP relative to control females. In contrast, a statistically significant increase in the mutation frequency of male mice exposed in utero to DEP was observed (2-fold; Fisher's exact p < 0.05). Thus, maternal exposure to DEP results in increased mutation in sperm during development.

    KW - ESTRs

    KW - DEP

    KW - Germline instability

    KW - Epigenetics

    U2 - 10.1016/j.mrfmmm.2011.04.007

    DO - 10.1016/j.mrfmmm.2011.04.007

    M3 - Journal article

    VL - 712

    SP - 55

    EP - 58

    JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

    JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

    SN - 0027-5107

    IS - 1-2

    ER -