Genomic characterization of enteroaggregative Escherichia coli from children in Mali

Background. Enteroaggregative Escherichia coli (EAEC) is a cause of epidemic and sporadic diarrhea, yet its roleas an enteric pathogen is not fully understood.Methods. We characterized 121 EAEC strains isolated in 2008 as part of a case-control study of moderate tosevere acute diarrhea among children 0–59 months of age in Bamako, Mali. We applied multiplex polymerase chainreaction and comparative genome hybridization to identify potential virulence factors among the EAEC strains,coupled with classification and regression tree modeling to reveal combinations of factors most strongly associatedwith illness.Results. The gene encoding the autotransporter protease SepA, originally described in Shigella species, was moststrongly associated with diarrhea among the EAEC strains tested (odds ratio, 5.6 [95% confidence interval, 1.92–16.17];P 5 .0006). In addition, we identified 3 gene combinations correlated with diarrhea: (1) a clonal group positive forsepA and a putative hemolysin; (2) a group harboring the EAST-1 enterotoxin and the flagellar type H33 but no otherpreviously identified EAEC virulence factor; and (3) a group carrying several of the typical EAEC virulence genes.Conclusion. Our data suggest that only a subset of EAEC strains are pathogenic in Mali and suggest that sepAmay serve as a valuable marker for the most virulent isolates.