Gene duplications and losses among vertebrate deoxyribonucleoside kinases of the non-TK1 Family

Zeeshan Mutahir, Louise Slot Christiansen, Anders R. Clausen, Martin Werner Berchtold, Zoran Gojkovic, Birgitte Munch-Petersen, Wolfgang Knecht, Jure Piskur

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides (dNs) and catalyze the rate limiting step of this salvage pathway by converting dNs into corresponding monophosphate forms. These enzymes serve as an excellent model to study duplicated genes and their evolutionary history. So far, among vertebrates only four mammalian dNKs have been studied for their substrate specificity and kinetic properties. However, some vertebrates, such as fish, frogs, and birds, apparently possess a duplicated homolog of deoxycytidine kinase (dCK). In this study, we characterized a family of dCK/deoxyguanosine kinase (dGK)-like enzymes from a frog Xenopus laevis and a bird Gallus gallus. We showed that X. laevis has a duplicated dCK gene and a dGK gene, whereas G. gallus has a duplicated dCK gene but has lost the dGK gene. We cloned, expressed, purified, and subsequently determined the kinetic parameters of the dCK/dGK enzymes encoded by these genes. The two dCK enzymes in G. gallus have broader substrate specificity than their human or X. laevis counterparts. Additionally, the duplicated dCK enzyme in G. gallus might have become mitochondria. Based on our study we postulate that changing and adapting substrate specificities and subcellular localization are likely the drivers behind the evolution of vertebrate dNKs
OriginalsprogEngelsk
TidsskriftNucleosides, Nucleotides and Nucleic Acids
Vol/bind35
Udgave nummer10-12
Sider (fra-til)677-690
Antal sider14
ISSN1525-7770
DOI
StatusUdgivet - 1 dec. 2016

Citer dette

Mutahir, Zeeshan ; Christiansen, Louise Slot ; Clausen, Anders R. ; Berchtold, Martin Werner ; Gojkovic, Zoran ; Munch-Petersen, Birgitte ; Knecht, Wolfgang ; Piskur, Jure. / Gene duplications and losses among vertebrate deoxyribonucleoside kinases of the non-TK1 Family. I: Nucleosides, Nucleotides and Nucleic Acids. 2016 ; Bind 35, Nr. 10-12. s. 677-690.
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abstract = "Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides (dNs) and catalyze the rate limiting step of this salvage pathway by converting dNs into corresponding monophosphate forms. These enzymes serve as an excellent model to study duplicated genes and their evolutionary history. So far, among vertebrates only four mammalian dNKs have been studied for their substrate specificity and kinetic properties. However, some vertebrates, such as fish, frogs, and birds, apparently possess a duplicated homolog of deoxycytidine kinase (dCK). In this study, we characterized a family of dCK/deoxyguanosine kinase (dGK)-like enzymes from a frog Xenopus laevis and a bird Gallus gallus. We showed that X. laevis has a duplicated dCK gene and a dGK gene, whereas G. gallus has a duplicated dCK gene but has lost the dGK gene. We cloned, expressed, purified, and subsequently determined the kinetic parameters of the dCK/dGK enzymes encoded by these genes. The two dCK enzymes in G. gallus have broader substrate specificity than their human or X. laevis counterparts. Additionally, the duplicated dCK enzyme in G. gallus might have become mitochondria. Based on our study we postulate that changing and adapting substrate specificities and subcellular localization are likely the drivers behind the evolution of vertebrate dNKs",
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author = "Zeeshan Mutahir and Christiansen, {Louise Slot} and Clausen, {Anders R.} and Berchtold, {Martin Werner} and Zoran Gojkovic and Birgitte Munch-Petersen and Wolfgang Knecht and Jure Piskur",
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Mutahir, Z, Christiansen, LS, Clausen, AR, Berchtold, MW, Gojkovic, Z, Munch-Petersen, B, Knecht, W & Piskur, J 2016, 'Gene duplications and losses among vertebrate deoxyribonucleoside kinases of the non-TK1 Family', Nucleosides, Nucleotides and Nucleic Acids, bind 35, nr. 10-12, s. 677-690. https://doi.org/10.1080/15257770.2016.1143557

Gene duplications and losses among vertebrate deoxyribonucleoside kinases of the non-TK1 Family. / Mutahir, Zeeshan; Christiansen, Louise Slot; Clausen, Anders R.; Berchtold, Martin Werner; Gojkovic, Zoran; Munch-Petersen, Birgitte; Knecht, Wolfgang; Piskur, Jure.

I: Nucleosides, Nucleotides and Nucleic Acids, Bind 35, Nr. 10-12, 01.12.2016, s. 677-690.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Gene duplications and losses among vertebrate deoxyribonucleoside kinases of the non-TK1 Family

AU - Mutahir, Zeeshan

AU - Christiansen, Louise Slot

AU - Clausen, Anders R.

AU - Berchtold, Martin Werner

AU - Gojkovic, Zoran

AU - Munch-Petersen, Birgitte

AU - Knecht, Wolfgang

AU - Piskur, Jure

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides (dNs) and catalyze the rate limiting step of this salvage pathway by converting dNs into corresponding monophosphate forms. These enzymes serve as an excellent model to study duplicated genes and their evolutionary history. So far, among vertebrates only four mammalian dNKs have been studied for their substrate specificity and kinetic properties. However, some vertebrates, such as fish, frogs, and birds, apparently possess a duplicated homolog of deoxycytidine kinase (dCK). In this study, we characterized a family of dCK/deoxyguanosine kinase (dGK)-like enzymes from a frog Xenopus laevis and a bird Gallus gallus. We showed that X. laevis has a duplicated dCK gene and a dGK gene, whereas G. gallus has a duplicated dCK gene but has lost the dGK gene. We cloned, expressed, purified, and subsequently determined the kinetic parameters of the dCK/dGK enzymes encoded by these genes. The two dCK enzymes in G. gallus have broader substrate specificity than their human or X. laevis counterparts. Additionally, the duplicated dCK enzyme in G. gallus might have become mitochondria. Based on our study we postulate that changing and adapting substrate specificities and subcellular localization are likely the drivers behind the evolution of vertebrate dNKs

AB - Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides (dNs) and catalyze the rate limiting step of this salvage pathway by converting dNs into corresponding monophosphate forms. These enzymes serve as an excellent model to study duplicated genes and their evolutionary history. So far, among vertebrates only four mammalian dNKs have been studied for their substrate specificity and kinetic properties. However, some vertebrates, such as fish, frogs, and birds, apparently possess a duplicated homolog of deoxycytidine kinase (dCK). In this study, we characterized a family of dCK/deoxyguanosine kinase (dGK)-like enzymes from a frog Xenopus laevis and a bird Gallus gallus. We showed that X. laevis has a duplicated dCK gene and a dGK gene, whereas G. gallus has a duplicated dCK gene but has lost the dGK gene. We cloned, expressed, purified, and subsequently determined the kinetic parameters of the dCK/dGK enzymes encoded by these genes. The two dCK enzymes in G. gallus have broader substrate specificity than their human or X. laevis counterparts. Additionally, the duplicated dCK enzyme in G. gallus might have become mitochondria. Based on our study we postulate that changing and adapting substrate specificities and subcellular localization are likely the drivers behind the evolution of vertebrate dNKs

KW - Gallus gallus

KW - Nucleoside salvage pathway

KW - Xenopus laevis

KW - deoxyribonucleosides

KW - evolution

U2 - 10.1080/15257770.2016.1143557

DO - 10.1080/15257770.2016.1143557

M3 - Journal article

VL - 35

SP - 677

EP - 690

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

SN - 1525-7770

IS - 10-12

ER -