Functional Mitochondria Are Important for the Effect of Resveratrol

Anne Lykkegaard Widlund, Kaushal Baral, Louise Torp Dalgaard, Ole Vang

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Resveratrol (Resv) is a polyphenol reported to modulate mitochondrial activity. The aim was to use HeLa and 143B cells to characterize the action of Resv on mitochondrial activity, cell size and proliferation using wild type (WT) and Rho 0 cells deficient in mitochondrial DNA. In both HeLa WT and Rho 0 cells, the oxygen consumption rate (OCR) was increased at 20 µM Resv after 24 h, whereas only a non-significant increase of OCR was observed in 143B WT cells. Resv decreased cell number concentration-dependently in both WT and Rho 0 cell types. An increased cell diameter was observed in HeLa WT, but not in Rho 0 when treated with Resv. Overall, the findings presented indicate that functional mitochondria are a prerequisite for cell enlargement by Resv.
OriginalsprogEngelsk
Artikelnummer847
TidsskriftMolecules
Vol/bind22
Udgave nummer5
ISSN1420-3049
DOI
StatusUdgivet - maj 2017

Citer dette

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abstract = "Resveratrol (Resv) is a polyphenol reported to modulate mitochondrial activity. The aim was to use HeLa and 143B cells to characterize the action of Resv on mitochondrial activity, cell size and proliferation using wild type (WT) and Rho 0 cells deficient in mitochondrial DNA. In both HeLa WT and Rho 0 cells, the oxygen consumption rate (OCR) was increased at 20 µM Resv after 24 h, whereas only a non-significant increase of OCR was observed in 143B WT cells. Resv decreased cell number concentration-dependently in both WT and Rho 0 cell types. An increased cell diameter was observed in HeLa WT, but not in Rho 0 when treated with Resv. Overall, the findings presented indicate that functional mitochondria are a prerequisite for cell enlargement by Resv.",
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author = "Widlund, {Anne Lykkegaard} and Kaushal Baral and Dalgaard, {Louise Torp} and Ole Vang",
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Functional Mitochondria Are Important for the Effect of Resveratrol. / Widlund, Anne Lykkegaard; Baral, Kaushal; Dalgaard, Louise Torp; Vang, Ole.

I: Molecules, Bind 22, Nr. 5, 847, 05.2017.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Functional Mitochondria Are Important for the Effect of Resveratrol

AU - Widlund, Anne Lykkegaard

AU - Baral, Kaushal

AU - Dalgaard, Louise Torp

AU - Vang, Ole

PY - 2017/5

Y1 - 2017/5

N2 - Resveratrol (Resv) is a polyphenol reported to modulate mitochondrial activity. The aim was to use HeLa and 143B cells to characterize the action of Resv on mitochondrial activity, cell size and proliferation using wild type (WT) and Rho 0 cells deficient in mitochondrial DNA. In both HeLa WT and Rho 0 cells, the oxygen consumption rate (OCR) was increased at 20 µM Resv after 24 h, whereas only a non-significant increase of OCR was observed in 143B WT cells. Resv decreased cell number concentration-dependently in both WT and Rho 0 cell types. An increased cell diameter was observed in HeLa WT, but not in Rho 0 when treated with Resv. Overall, the findings presented indicate that functional mitochondria are a prerequisite for cell enlargement by Resv.

AB - Resveratrol (Resv) is a polyphenol reported to modulate mitochondrial activity. The aim was to use HeLa and 143B cells to characterize the action of Resv on mitochondrial activity, cell size and proliferation using wild type (WT) and Rho 0 cells deficient in mitochondrial DNA. In both HeLa WT and Rho 0 cells, the oxygen consumption rate (OCR) was increased at 20 µM Resv after 24 h, whereas only a non-significant increase of OCR was observed in 143B WT cells. Resv decreased cell number concentration-dependently in both WT and Rho 0 cell types. An increased cell diameter was observed in HeLa WT, but not in Rho 0 when treated with Resv. Overall, the findings presented indicate that functional mitochondria are a prerequisite for cell enlargement by Resv.

KW - 143B

KW - HeLa

KW - Rho 0

KW - mitochondria

KW - oxidative stress

KW - oxygen consumption rate

KW - resveratrol

KW - wild type

U2 - 10.3390/molecules22050847

DO - 10.3390/molecules22050847

M3 - Journal article

VL - 22

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 5

M1 - 847

ER -