Gasification biochar has limited effects on functional and structural diversity of soil microbial communities in a temperate agroecosystem

Bidragets oversatte titel: Forgasningsbiokoks har begrænset effekt på den funktionelle og strukturelle diversitet af jordens mikrobielle samfund i et tempereret agroøkosystem

Valentina Imparato, Veronika Hansen, Susana Santos, Tue Kjærgaard Nielsen, Laura Giagnoni, Henrik Hauggaard-Nielsen, Anders Johansen, Giancarlo Renella, Anne Winding

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Biochar may enhance soil fertility and carbon (C) sequestration but there is still a lack of comprehensive understanding of its effects on soil microbial communities and functioning. This study tested the differential effects of two doses (6-8 and 0.8-1.4 t ha-1 for High and Low doses, respectively) of wheat straw gasification biochar (GBC) and fresh straw incorporated as soil amendments into an agricultural field in Denmark. Soils were analysed three months after the amendments for pH, total organic matter, microbial biomass (ATP), ten enzymatic activities, catabolic potential by substrate-induced respiration (MicroResp™), soil toxicity test (BioTox™) and bacterial community structure (Illumina 16S rRNA gene sequencing). No significant effect of biochar treatment was observed regarding ATP content, catabolic community profiles and soil toxicity. The higher dose of GBC increased phenol oxidase activity and soil pH, and decreased the cellulase activity. No major effect of high dose GBC was observed on the soil community diversity, and only minor effect on the community composition, with an increase in the relative abundance of a single OTU associated with Acidobacteria_Gp16. Addition of low dose of GBC caused an increase in the relative abundance of the rare members in the microbial communities thus increasing the diversity of soil microorganisms. A comparable effect was observed with the addition of fresh straw. Overall, our results indicated that GBC as soil amendment had a limited effect on the functional and structural diversity of soil microbial communities in a Danish temperate agroecosystem.
OriginalsprogEngelsk
TidsskriftSoil Biology & Biochemistry
Vol/bind99
Sider (fra-til)128-136
Antal sider9
ISSN0038-0717
DOI
StatusUdgivet - 2016

Emneord

  • Biochar amendment
  • rare microbiome
  • toxicity test
  • microbial activity
  • MicroResp
  • 16S illumina sequencing

Citer dette

Imparato, Valentina ; Hansen, Veronika ; Santos, Susana ; Nielsen, Tue Kjærgaard ; Giagnoni, Laura ; Hauggaard-Nielsen, Henrik ; Johansen, Anders ; Renella, Giancarlo ; Winding, Anne. / Gasification biochar has limited effects on functional and structural diversity of soil microbial communities in a temperate agroecosystem. I: Soil Biology & Biochemistry. 2016 ; Bind 99. s. 128-136.
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abstract = "Biochar may enhance soil fertility and carbon (C) sequestration but there is still a lack of comprehensive understanding of its effects on soil microbial communities and functioning. This study tested the differential effects of two doses (6-8 and 0.8-1.4 t ha-1 for High and Low doses, respectively) of wheat straw gasification biochar (GBC) and fresh straw incorporated as soil amendments into an agricultural field in Denmark. Soils were analysed three months after the amendments for pH, total organic matter, microbial biomass (ATP), ten enzymatic activities, catabolic potential by substrate-induced respiration (MicroResp™), soil toxicity test (BioTox™) and bacterial community structure (Illumina 16S rRNA gene sequencing). No significant effect of biochar treatment was observed regarding ATP content, catabolic community profiles and soil toxicity. The higher dose of GBC increased phenol oxidase activity and soil pH, and decreased the cellulase activity. No major effect of high dose GBC was observed on the soil community diversity, and only minor effect on the community composition, with an increase in the relative abundance of a single OTU associated with Acidobacteria_Gp16. Addition of low dose of GBC caused an increase in the relative abundance of the rare members in the microbial communities thus increasing the diversity of soil microorganisms. A comparable effect was observed with the addition of fresh straw. Overall, our results indicated that GBC as soil amendment had a limited effect on the functional and structural diversity of soil microbial communities in a Danish temperate agroecosystem",
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Gasification biochar has limited effects on functional and structural diversity of soil microbial communities in a temperate agroecosystem. / Imparato, Valentina; Hansen, Veronika; Santos, Susana; Nielsen, Tue Kjærgaard; Giagnoni, Laura; Hauggaard-Nielsen, Henrik; Johansen, Anders; Renella, Giancarlo; Winding, Anne.

I: Soil Biology & Biochemistry, Bind 99, 2016, s. 128-136.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Gasification biochar has limited effects on functional and structural diversity of soil microbial communities in a temperate agroecosystem

AU - Imparato, Valentina

AU - Hansen, Veronika

AU - Santos, Susana

AU - Nielsen, Tue Kjærgaard

AU - Giagnoni, Laura

AU - Hauggaard-Nielsen, Henrik

AU - Johansen, Anders

AU - Renella, Giancarlo

AU - Winding, Anne

PY - 2016

Y1 - 2016

N2 - Biochar may enhance soil fertility and carbon (C) sequestration but there is still a lack of comprehensive understanding of its effects on soil microbial communities and functioning. This study tested the differential effects of two doses (6-8 and 0.8-1.4 t ha-1 for High and Low doses, respectively) of wheat straw gasification biochar (GBC) and fresh straw incorporated as soil amendments into an agricultural field in Denmark. Soils were analysed three months after the amendments for pH, total organic matter, microbial biomass (ATP), ten enzymatic activities, catabolic potential by substrate-induced respiration (MicroResp™), soil toxicity test (BioTox™) and bacterial community structure (Illumina 16S rRNA gene sequencing). No significant effect of biochar treatment was observed regarding ATP content, catabolic community profiles and soil toxicity. The higher dose of GBC increased phenol oxidase activity and soil pH, and decreased the cellulase activity. No major effect of high dose GBC was observed on the soil community diversity, and only minor effect on the community composition, with an increase in the relative abundance of a single OTU associated with Acidobacteria_Gp16. Addition of low dose of GBC caused an increase in the relative abundance of the rare members in the microbial communities thus increasing the diversity of soil microorganisms. A comparable effect was observed with the addition of fresh straw. Overall, our results indicated that GBC as soil amendment had a limited effect on the functional and structural diversity of soil microbial communities in a Danish temperate agroecosystem

AB - Biochar may enhance soil fertility and carbon (C) sequestration but there is still a lack of comprehensive understanding of its effects on soil microbial communities and functioning. This study tested the differential effects of two doses (6-8 and 0.8-1.4 t ha-1 for High and Low doses, respectively) of wheat straw gasification biochar (GBC) and fresh straw incorporated as soil amendments into an agricultural field in Denmark. Soils were analysed three months after the amendments for pH, total organic matter, microbial biomass (ATP), ten enzymatic activities, catabolic potential by substrate-induced respiration (MicroResp™), soil toxicity test (BioTox™) and bacterial community structure (Illumina 16S rRNA gene sequencing). No significant effect of biochar treatment was observed regarding ATP content, catabolic community profiles and soil toxicity. The higher dose of GBC increased phenol oxidase activity and soil pH, and decreased the cellulase activity. No major effect of high dose GBC was observed on the soil community diversity, and only minor effect on the community composition, with an increase in the relative abundance of a single OTU associated with Acidobacteria_Gp16. Addition of low dose of GBC caused an increase in the relative abundance of the rare members in the microbial communities thus increasing the diversity of soil microorganisms. A comparable effect was observed with the addition of fresh straw. Overall, our results indicated that GBC as soil amendment had a limited effect on the functional and structural diversity of soil microbial communities in a Danish temperate agroecosystem

KW - Biochar amendment

KW - rare microbiome

KW - toxicity test

KW - microbial activity

KW - MicroResp

KW - 16S illumina sequencing

KW - 16S illumina sequencing

KW - MicroResp

KW - microbial activity

KW - Biochar amendment

KW - rare microbiome

KW - toxicity test

U2 - 10.1016/j.soilbio.2016.05.004

DO - 10.1016/j.soilbio.2016.05.004

M3 - Journal article

VL - 99

SP - 128

EP - 136

JO - Soil Biology & Biochemistry

JF - Soil Biology & Biochemistry

SN - 0038-0717

ER -