Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo

Pia Nyeng*, Gelo Victoriano Dela Cruz, Henrik Semb

*Corresponding author

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstract

We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).

OriginalsprogEngelsk
Artikelnummer100636
TidsskriftSTAR protocols
Vol/bind2
Udgave nummer3
Sider (fra-til)100636
Antal sider1
ISSN2666-1667
DOI
StatusUdgivet - 17 sep. 2021

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