Expression of prostaglandin synthases (pgds and pges) during zebrafish gonadal differentiation

Anne Jørgensen, John E. Nielsen, Betina F. Nielsen, Jane E. Morthorst, Poul Bjerregaard, Henrik Leffers

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    The present study aimed at elucidating whether the expression pattern of the membrane bound form of prostaglandin E-2 synthase (pges) and especially the lipocalin-type prostaglandin D-2 synthase (pgds) indicates involvement in gonadal sex differentiation in zebrafish as has previously been found in other species In mice and chicken, the lipocalin-type Pgds is specifically expressed in pre-Sertoli cells just after Sty and Sox9 and is involved in masculinisation of the developing testis. Furthermore, Pges are implicated in female reproduction including follicular development and ovulation In this study, a sexually dimorphic expression of pgds was found in gonads of adult zebrafish with expression in testis but not in ovaries. To determine whether the sex-specific expression pattern of pgds was present in gonads of juvenile zebrafish and therefore could be an early marker of sex in zebrafish, we microdissected gonads from four randomly selected individual zebrafish for every second day in the period 2-20 days post hatch (dph) and 0-1 dph The temporal expression of pgds and pges was investigated in the microdissected gonads, however, no differential expression that could indicate sex-specific difference between individual juvenile zebrafish was observed (C) 2010 Elsevier Inc All rights reserved
    OriginalsprogEngelsk
    TidsskriftComparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
    Vol/bind157
    Udgave nummer1
    Sider (fra-til)102-108
    ISSN1095-6433
    DOI
    StatusUdgivet - 2010

    Citer dette

    Jørgensen, Anne ; Nielsen, John E. ; Nielsen, Betina F. ; Morthorst, Jane E. ; Bjerregaard, Poul ; Leffers, Henrik. / Expression of prostaglandin synthases (pgds and pges) during zebrafish gonadal differentiation. I: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology. 2010 ; Bind 157, Nr. 1. s. 102-108.
    @article{3b90b82d0b484517a0aecf9b8d347973,
    title = "Expression of prostaglandin synthases (pgds and pges) during zebrafish gonadal differentiation",
    abstract = "The present study aimed at elucidating whether the expression pattern of the membrane bound form of prostaglandin E-2 synthase (pges) and especially the lipocalin-type prostaglandin D-2 synthase (pgds) indicates involvement in gonadal sex differentiation in zebrafish as has previously been found in other species In mice and chicken, the lipocalin-type Pgds is specifically expressed in pre-Sertoli cells just after Sty and Sox9 and is involved in masculinisation of the developing testis. Furthermore, Pges are implicated in female reproduction including follicular development and ovulation In this study, a sexually dimorphic expression of pgds was found in gonads of adult zebrafish with expression in testis but not in ovaries. To determine whether the sex-specific expression pattern of pgds was present in gonads of juvenile zebrafish and therefore could be an early marker of sex in zebrafish, we microdissected gonads from four randomly selected individual zebrafish for every second day in the period 2-20 days post hatch (dph) and 0-1 dph The temporal expression of pgds and pges was investigated in the microdissected gonads, however, no differential expression that could indicate sex-specific difference between individual juvenile zebrafish was observed (C) 2010 Elsevier Inc All rights reserved",
    keywords = "Danio rerio, Gonadal differentiation, In situ hybridisation, Laser capture microdissection, RT-PCR, pgds, pges, vasa",
    author = "Anne J{\o}rgensen and Nielsen, {John E.} and Nielsen, {Betina F.} and Morthorst, {Jane E.} and Poul Bjerregaard and Henrik Leffers",
    year = "2010",
    doi = "10.1016/j.cbpa.2010.03.014",
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    Expression of prostaglandin synthases (pgds and pges) during zebrafish gonadal differentiation. / Jørgensen, Anne; Nielsen, John E.; Nielsen, Betina F.; Morthorst, Jane E.; Bjerregaard, Poul; Leffers, Henrik.

    I: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, Bind 157, Nr. 1, 2010, s. 102-108.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Expression of prostaglandin synthases (pgds and pges) during zebrafish gonadal differentiation

    AU - Jørgensen, Anne

    AU - Nielsen, John E.

    AU - Nielsen, Betina F.

    AU - Morthorst, Jane E.

    AU - Bjerregaard, Poul

    AU - Leffers, Henrik

    PY - 2010

    Y1 - 2010

    N2 - The present study aimed at elucidating whether the expression pattern of the membrane bound form of prostaglandin E-2 synthase (pges) and especially the lipocalin-type prostaglandin D-2 synthase (pgds) indicates involvement in gonadal sex differentiation in zebrafish as has previously been found in other species In mice and chicken, the lipocalin-type Pgds is specifically expressed in pre-Sertoli cells just after Sty and Sox9 and is involved in masculinisation of the developing testis. Furthermore, Pges are implicated in female reproduction including follicular development and ovulation In this study, a sexually dimorphic expression of pgds was found in gonads of adult zebrafish with expression in testis but not in ovaries. To determine whether the sex-specific expression pattern of pgds was present in gonads of juvenile zebrafish and therefore could be an early marker of sex in zebrafish, we microdissected gonads from four randomly selected individual zebrafish for every second day in the period 2-20 days post hatch (dph) and 0-1 dph The temporal expression of pgds and pges was investigated in the microdissected gonads, however, no differential expression that could indicate sex-specific difference between individual juvenile zebrafish was observed (C) 2010 Elsevier Inc All rights reserved

    AB - The present study aimed at elucidating whether the expression pattern of the membrane bound form of prostaglandin E-2 synthase (pges) and especially the lipocalin-type prostaglandin D-2 synthase (pgds) indicates involvement in gonadal sex differentiation in zebrafish as has previously been found in other species In mice and chicken, the lipocalin-type Pgds is specifically expressed in pre-Sertoli cells just after Sty and Sox9 and is involved in masculinisation of the developing testis. Furthermore, Pges are implicated in female reproduction including follicular development and ovulation In this study, a sexually dimorphic expression of pgds was found in gonads of adult zebrafish with expression in testis but not in ovaries. To determine whether the sex-specific expression pattern of pgds was present in gonads of juvenile zebrafish and therefore could be an early marker of sex in zebrafish, we microdissected gonads from four randomly selected individual zebrafish for every second day in the period 2-20 days post hatch (dph) and 0-1 dph The temporal expression of pgds and pges was investigated in the microdissected gonads, however, no differential expression that could indicate sex-specific difference between individual juvenile zebrafish was observed (C) 2010 Elsevier Inc All rights reserved

    KW - Danio rerio

    KW - Gonadal differentiation

    KW - In situ hybridisation

    KW - Laser capture microdissection

    KW - RT-PCR

    KW - pgds

    KW - pges

    KW - vasa

    U2 - 10.1016/j.cbpa.2010.03.014

    DO - 10.1016/j.cbpa.2010.03.014

    M3 - Journal article

    VL - 157

    SP - 102

    EP - 108

    JO - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

    JF - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

    SN - 1095-6433

    IS - 1

    ER -