Expression of miR-206 in human islets and its role in glucokinase regulation

Mugdha Joglekar, Wilson W.K. Wong, C.L. Maynard, M.R. Umrani, D. Martin, T. Loudovaris, H.E. Thomas, Louise Torp Dalgaard, Anandwardhan Awadhoot Hardikar

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Inappropriate insulin secretion from β-cells is considered as an early sign of impaired glucose tolerance and type 2 diabetes (T2D). Glucokinase (GCK) is an important enzyme that regulates glucose metabolism and ensures that the normal circulating glucose concentrations are maintained. GCK expression is induced by glucose and regulated via transcription factors and regulatory proteins. Recently, microRNA-206 (miR-206) was reported to regulate GCK and alter glucose tolerance in normal and high-fat diet-fed mice. Although the study findings have implications for human diabetes, studies in human islets are lacking. Here, we analyze human islets from individuals without or with T2D, using TaqMan-based real-time qPCR at the tissue (isolated islet) level as well as at single cell resolution, to assess the relationship between miR-206 and GCK expression in normal and T2D human islets. Our data suggest that, unlike mouse islets, human islets do not exhibit any correlation between miR-206 and GCK transcripts. These data implicate the need for further studies aimed toward exploring its potential role(s) in human islets.
OriginalsprogEngelsk
TidsskriftAmerican Journal of Physiology: Endocrinology and Metabolism
Vol/bind315
Udgave nummer4
Sider (fra-til)634-637
Antal sider4
ISSN0193-1849
DOI
StatusUdgivet - 2018

Citer dette

Joglekar, Mugdha ; Wong, Wilson W.K. ; Maynard, C.L. ; Umrani, M.R. ; Martin, D. ; Loudovaris, T. ; Thomas, H.E. ; Dalgaard, Louise Torp ; Hardikar, Anandwardhan Awadhoot. / Expression of miR-206 in human islets and its role in glucokinase regulation. I: American Journal of Physiology: Endocrinology and Metabolism. 2018 ; Bind 315, Nr. 4. s. 634-637.
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title = "Expression of miR-206 in human islets and its role in glucokinase regulation",
abstract = "Inappropriate insulin secretion from β-cells is considered as an early sign of impaired glucose tolerance and type 2 diabetes (T2D). Glucokinase (GCK) is an important enzyme that regulates glucose metabolism and ensures that the normal circulating glucose concentrations are maintained. GCK expression is induced by glucose and regulated via transcription factors and regulatory proteins. Recently, microRNA-206 (miR-206) was reported to regulate GCK and alter glucose tolerance in normal and high-fat diet-fed mice. Although the study findings have implications for human diabetes, studies in human islets are lacking. Here, we analyze human islets from individuals without or with T2D, using TaqMan-based real-time qPCR at the tissue (isolated islet) level as well as at single cell resolution, to assess the relationship between miR-206 and GCK expression in normal and T2D human islets. Our data suggest that, unlike mouse islets, human islets do not exhibit any correlation between miR-206 and GCK transcripts. These data implicate the need for further studies aimed toward exploring its potential role(s) in human islets.",
author = "Mugdha Joglekar and Wong, {Wilson W.K.} and C.L. Maynard and M.R. Umrani and D. Martin and T. Loudovaris and H.E. Thomas and Dalgaard, {Louise Torp} and Hardikar, {Anandwardhan Awadhoot}",
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Expression of miR-206 in human islets and its role in glucokinase regulation. / Joglekar, Mugdha; Wong, Wilson W.K.; Maynard, C.L.; Umrani, M.R.; Martin, D.; Loudovaris, T.; Thomas, H.E.; Dalgaard, Louise Torp; Hardikar, Anandwardhan Awadhoot.

I: American Journal of Physiology: Endocrinology and Metabolism, Bind 315, Nr. 4, 2018, s. 634-637.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Expression of miR-206 in human islets and its role in glucokinase regulation

AU - Joglekar, Mugdha

AU - Wong, Wilson W.K.

AU - Maynard, C.L.

AU - Umrani, M.R.

AU - Martin, D.

AU - Loudovaris, T.

AU - Thomas, H.E.

AU - Dalgaard, Louise Torp

AU - Hardikar, Anandwardhan Awadhoot

PY - 2018

Y1 - 2018

N2 - Inappropriate insulin secretion from β-cells is considered as an early sign of impaired glucose tolerance and type 2 diabetes (T2D). Glucokinase (GCK) is an important enzyme that regulates glucose metabolism and ensures that the normal circulating glucose concentrations are maintained. GCK expression is induced by glucose and regulated via transcription factors and regulatory proteins. Recently, microRNA-206 (miR-206) was reported to regulate GCK and alter glucose tolerance in normal and high-fat diet-fed mice. Although the study findings have implications for human diabetes, studies in human islets are lacking. Here, we analyze human islets from individuals without or with T2D, using TaqMan-based real-time qPCR at the tissue (isolated islet) level as well as at single cell resolution, to assess the relationship between miR-206 and GCK expression in normal and T2D human islets. Our data suggest that, unlike mouse islets, human islets do not exhibit any correlation between miR-206 and GCK transcripts. These data implicate the need for further studies aimed toward exploring its potential role(s) in human islets.

AB - Inappropriate insulin secretion from β-cells is considered as an early sign of impaired glucose tolerance and type 2 diabetes (T2D). Glucokinase (GCK) is an important enzyme that regulates glucose metabolism and ensures that the normal circulating glucose concentrations are maintained. GCK expression is induced by glucose and regulated via transcription factors and regulatory proteins. Recently, microRNA-206 (miR-206) was reported to regulate GCK and alter glucose tolerance in normal and high-fat diet-fed mice. Although the study findings have implications for human diabetes, studies in human islets are lacking. Here, we analyze human islets from individuals without or with T2D, using TaqMan-based real-time qPCR at the tissue (isolated islet) level as well as at single cell resolution, to assess the relationship between miR-206 and GCK expression in normal and T2D human islets. Our data suggest that, unlike mouse islets, human islets do not exhibit any correlation between miR-206 and GCK transcripts. These data implicate the need for further studies aimed toward exploring its potential role(s) in human islets.

U2 - 10.1152/ajpendo.00152.2018

DO - 10.1152/ajpendo.00152.2018

M3 - Journal article

VL - 315

SP - 634

EP - 637

JO - American Journal of Physiology: Endocrinology and Metabolism

JF - American Journal of Physiology: Endocrinology and Metabolism

SN - 0193-1849

IS - 4

ER -