Expression of hsp70 and ferritin in embryos of the copepod Acartia tonsa (Dana) during transition between subitaneous and quiescent state

Birgitte Nilsson, Per Meyer Jepsen, Kim Rewitz, Benni Winding Hansen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Subitaneous eggs of the neritic calanoid copepod Acartia tonsa (Dana) are capable of entering a resting state called quiescence to overcome adverse environmental conditions. Although physiological changes associated with this transition have been described, the molecular mechanisms are thus far not studied in any calanoid copepod. Two stress-related proteins, hsp70 and ferritin, were cloned from A. tonsa and their expression determined in embryos of A. tonsa during transition between subitaneous and quiescence eggs. Expression of hsp70 remained low during quiescence despite environmental stress. However, ferritin expression exhibited a strong increase from days 3 to 5 of quiescence followed by a declined. After 2 weeks of quiescence, development of the embryos recovered by the addition of oxygen and increased temperature. The expression of both genes increased 222-fold for hsp70 and 52-fold for ferritin during the recovery phase toward hatching. This suggests that ferritin is a protein needed when embryos of A. tonsa enter quiescence. Both hsp70 and ferritin are required during recovery from quiescent to subitaneous state when embryogenesis continues toward egg hatching.
OriginalsprogEngelsk
TidsskriftJournal of Plankton Research
Vol/bind36
Udgave nummer2
Sider (fra-til)513-522
Antal sider10
ISSN0142-7873
DOI
StatusUdgivet - 26 nov. 2014

Emneord

  • Acartia tonsa
  • quiescence
  • hsp70
  • gene expression
  • copepod eggs

Citer dette

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title = "Expression of hsp70 and ferritin in embryos of the copepod Acartia tonsa (Dana) during transition between subitaneous and quiescent state",
abstract = "Subitaneous eggs of the neritic calanoid copepod Acartia tonsa (Dana) are capable of entering a resting state called quiescence to overcome adverse environmental conditions. Although physiological changes associated with this transition have been described, the molecular mechanisms are thus far not studied in any calanoid copepod. Two stress-related proteins, hsp70 and ferritin, were cloned from A. tonsa and their expression determined in embryos of A. tonsa during transition between subitaneous and quiescence eggs. Expression of hsp70 remained low during quiescence despite environmental stress. However, ferritin expression exhibited a strong increase from days 3 to 5 of quiescence followed by a declined. After 2 weeks of quiescence, development of the embryos recovered by the addition of oxygen and increased temperature. The expression of both genes increased 222-fold for hsp70 and 52-fold for ferritin during the recovery phase toward hatching. This suggests that ferritin is a protein needed when embryos of A. tonsa enter quiescence. Both hsp70 and ferritin are required during recovery from quiescent to subitaneous state when embryogenesis continues toward egg hatching.",
keywords = "Acartia tonsa , quiescence, hsp70, gene expression, copepod eggs",
author = "Birgitte Nilsson and Jepsen, {Per Meyer} and Kim Rewitz and Hansen, {Benni Winding}",
year = "2014",
month = "11",
day = "26",
doi = "10.1093/plankt/fbt099",
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volume = "36",
pages = "513--522",
journal = "Journal of Plankton Research",
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Expression of hsp70 and ferritin in embryos of the copepod Acartia tonsa (Dana) during transition between subitaneous and quiescent state. / Nilsson, Birgitte; Jepsen, Per Meyer; Rewitz, Kim; Hansen, Benni Winding.

I: Journal of Plankton Research, Bind 36, Nr. 2, 26.11.2014, s. 513-522.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Expression of hsp70 and ferritin in embryos of the copepod Acartia tonsa (Dana) during transition between subitaneous and quiescent state

AU - Nilsson, Birgitte

AU - Jepsen, Per Meyer

AU - Rewitz, Kim

AU - Hansen, Benni Winding

PY - 2014/11/26

Y1 - 2014/11/26

N2 - Subitaneous eggs of the neritic calanoid copepod Acartia tonsa (Dana) are capable of entering a resting state called quiescence to overcome adverse environmental conditions. Although physiological changes associated with this transition have been described, the molecular mechanisms are thus far not studied in any calanoid copepod. Two stress-related proteins, hsp70 and ferritin, were cloned from A. tonsa and their expression determined in embryos of A. tonsa during transition between subitaneous and quiescence eggs. Expression of hsp70 remained low during quiescence despite environmental stress. However, ferritin expression exhibited a strong increase from days 3 to 5 of quiescence followed by a declined. After 2 weeks of quiescence, development of the embryos recovered by the addition of oxygen and increased temperature. The expression of both genes increased 222-fold for hsp70 and 52-fold for ferritin during the recovery phase toward hatching. This suggests that ferritin is a protein needed when embryos of A. tonsa enter quiescence. Both hsp70 and ferritin are required during recovery from quiescent to subitaneous state when embryogenesis continues toward egg hatching.

AB - Subitaneous eggs of the neritic calanoid copepod Acartia tonsa (Dana) are capable of entering a resting state called quiescence to overcome adverse environmental conditions. Although physiological changes associated with this transition have been described, the molecular mechanisms are thus far not studied in any calanoid copepod. Two stress-related proteins, hsp70 and ferritin, were cloned from A. tonsa and their expression determined in embryos of A. tonsa during transition between subitaneous and quiescence eggs. Expression of hsp70 remained low during quiescence despite environmental stress. However, ferritin expression exhibited a strong increase from days 3 to 5 of quiescence followed by a declined. After 2 weeks of quiescence, development of the embryos recovered by the addition of oxygen and increased temperature. The expression of both genes increased 222-fold for hsp70 and 52-fold for ferritin during the recovery phase toward hatching. This suggests that ferritin is a protein needed when embryos of A. tonsa enter quiescence. Both hsp70 and ferritin are required during recovery from quiescent to subitaneous state when embryogenesis continues toward egg hatching.

KW - Acartia tonsa

KW - quiescence

KW - hsp70

KW - gene expression

KW - copepod eggs

U2 - 10.1093/plankt/fbt099

DO - 10.1093/plankt/fbt099

M3 - Journal article

VL - 36

SP - 513

EP - 522

JO - Journal of Plankton Research

JF - Journal of Plankton Research

SN - 0142-7873

IS - 2

ER -