TY - JOUR
T1 - Experimental Oesophagostomum dentatum infection in the pig
T2 - Worm populations resulting from single infections with three doses of larvae
AU - Christensen, C. M.
AU - Barnes, E. H.
AU - Nansen, P.
AU - Roepstorff, A.
AU - Slotved, H. C.
N1 - Funding Information:
Acknowledgements-Mr T. Olsen, Danish Pig Producers and Slaughterhouses Organization (Sjaelland II) is kindly acknowledged for taking good care of the animals. MS B. V. Ssnderby and MS P. Webster are thanked for their technical assistance in the laboratory. The Danish National Research Foundation is acknowledged for the financial support of this and concurrent projects under the auspices of The Danish Centre for Experimental Parasitology. The pigs used in this experiment were treated in accordance with animal ethics laws of Denmark (experimental animal permit no. 1994-101-115).
PY - 1995/12
Y1 - 1995/12
N2 - This report describes the effect of different dose levels of infection upon worm burdens and development and fecundity of the parasites. Three groups each of 40, 9-week-old, helminth naïve pigs were inoculated once with either 2000 (group A), 20,000 (group B), or 200,000 (group C) infective third stage larvae of Oesophagostomum dentatum. Subgroups of 5 pigs from each major group were killed 3, 6, 11, 14, 18, 25, 34 and 47 days post inoculation (p.i.) and the large intestinal worm burdens were determined. Faecal egg counts were determined at frequent intervals after day 13 p.i. There were no overt clinical signs of gastrointestinal helminthosis during the experiment. Faecal egg counts became positive in groups A and B at around day 19 p.i., whereas most pigs in the high dose group C did not have positive egg counts until day 27-33 p.i. and some pigs remained with zero egg counts until the end of the study. Throughout the experiment the worm populations in group C consisted mainly of immature larval stages, while those in groups A and B were predominantly adult stages after days 14-18. Adult worms from the low dose group A were significantly longer than those from group C. At high population densities, stunted development of worms and reduced fecundity among female worms were found. Furthermore, there was a tendency for the distribution of the worms within the intestine to be altered with increasing population size.
AB - This report describes the effect of different dose levels of infection upon worm burdens and development and fecundity of the parasites. Three groups each of 40, 9-week-old, helminth naïve pigs were inoculated once with either 2000 (group A), 20,000 (group B), or 200,000 (group C) infective third stage larvae of Oesophagostomum dentatum. Subgroups of 5 pigs from each major group were killed 3, 6, 11, 14, 18, 25, 34 and 47 days post inoculation (p.i.) and the large intestinal worm burdens were determined. Faecal egg counts were determined at frequent intervals after day 13 p.i. There were no overt clinical signs of gastrointestinal helminthosis during the experiment. Faecal egg counts became positive in groups A and B at around day 19 p.i., whereas most pigs in the high dose group C did not have positive egg counts until day 27-33 p.i. and some pigs remained with zero egg counts until the end of the study. Throughout the experiment the worm populations in group C consisted mainly of immature larval stages, while those in groups A and B were predominantly adult stages after days 14-18. Adult worms from the low dose group A were significantly longer than those from group C. At high population densities, stunted development of worms and reduced fecundity among female worms were found. Furthermore, there was a tendency for the distribution of the worms within the intestine to be altered with increasing population size.
KW - dose dependency
KW - experimental infection
KW - fecundity
KW - nematode
KW - Oesophagostomum dentatum
KW - pig
KW - population
KW - stunted development
KW - worm length
UR - http://www.scopus.com/inward/record.url?scp=0028876474&partnerID=8YFLogxK
U2 - 10.1016/0020-7519(95)00085-2
DO - 10.1016/0020-7519(95)00085-2
M3 - Journal article
C2 - 8719962
AN - SCOPUS:0028876474
SN - 0020-7519
VL - 25
SP - 1491
EP - 1498
JO - International Journal for Parasitology
JF - International Journal for Parasitology
IS - 12
ER -