Estimating the rate of plasmid transfer: An end-point method

Lone Simonsen, D. M. Gordon, F. M. Stewart, B. R. Levin

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

We describe a method for determining the rate parameter of conjugative plasmid transfer that is based on single
estimates of donor, recipient and transconjugant densities and the growth rate in exponential phase of the mating
culture. The formula for estimating the plasmid transfer rate, y, was derived from a mathematical model describing
cell growth and plasmid transfer in batch culture. Computer simulations were used to explore the sensitivity of this
method to the realities of bacterial life, such as growth rate differences, plasmid segregation and transitory
derepression of pilus synthesis. As predicted by the theory, mating experiments with the plasmid Rl in Eschericlria
coli K12 demonstrated that the estimate y is unaffected by cell density, donocrecipient ratio and mating time.
Unlike previous techniques, our method allows us to investigate the effect of environmental factors on plasmid
transfer rates when these factors also influence population growth rates. To illustrate this, we examined the effect
of temperature on the rate of plasmid transfer.
OriginalsprogEngelsk
TidsskriftJournal of General Microbiology
Vol/bind136
Udgave nummer11
Antal sider7
DOI
StatusUdgivet - 1990
Udgivet eksterntJa

Citer dette

Simonsen, Lone ; Gordon, D. M. ; Stewart, F. M. ; Levin, B. R. / Estimating the rate of plasmid transfer : An end-point method. I: Journal of General Microbiology. 1990 ; Bind 136, Nr. 11.
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abstract = "We describe a method for determining the rate parameter of conjugative plasmid transfer that is based on singleestimates of donor, recipient and transconjugant densities and the growth rate in exponential phase of the matingculture. The formula for estimating the plasmid transfer rate, y, was derived from a mathematical model describingcell growth and plasmid transfer in batch culture. Computer simulations were used to explore the sensitivity of thismethod to the realities of bacterial life, such as growth rate differences, plasmid segregation and transitoryderepression of pilus synthesis. As predicted by the theory, mating experiments with the plasmid Rl in Eschericlriacoli K12 demonstrated that the estimate y is unaffected by cell density, donocrecipient ratio and mating time.Unlike previous techniques, our method allows us to investigate the effect of environmental factors on plasmidtransfer rates when these factors also influence population growth rates. To illustrate this, we examined the effectof temperature on the rate of plasmid transfer.",
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Estimating the rate of plasmid transfer : An end-point method. / Simonsen, Lone; Gordon, D. M.; Stewart, F. M.; Levin, B. R.

I: Journal of General Microbiology, Bind 136, Nr. 11, 1990.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Estimating the rate of plasmid transfer

T2 - An end-point method

AU - Simonsen, Lone

AU - Gordon, D. M.

AU - Stewart, F. M.

AU - Levin, B. R.

PY - 1990

Y1 - 1990

N2 - We describe a method for determining the rate parameter of conjugative plasmid transfer that is based on singleestimates of donor, recipient and transconjugant densities and the growth rate in exponential phase of the matingculture. The formula for estimating the plasmid transfer rate, y, was derived from a mathematical model describingcell growth and plasmid transfer in batch culture. Computer simulations were used to explore the sensitivity of thismethod to the realities of bacterial life, such as growth rate differences, plasmid segregation and transitoryderepression of pilus synthesis. As predicted by the theory, mating experiments with the plasmid Rl in Eschericlriacoli K12 demonstrated that the estimate y is unaffected by cell density, donocrecipient ratio and mating time.Unlike previous techniques, our method allows us to investigate the effect of environmental factors on plasmidtransfer rates when these factors also influence population growth rates. To illustrate this, we examined the effectof temperature on the rate of plasmid transfer.

AB - We describe a method for determining the rate parameter of conjugative plasmid transfer that is based on singleestimates of donor, recipient and transconjugant densities and the growth rate in exponential phase of the matingculture. The formula for estimating the plasmid transfer rate, y, was derived from a mathematical model describingcell growth and plasmid transfer in batch culture. Computer simulations were used to explore the sensitivity of thismethod to the realities of bacterial life, such as growth rate differences, plasmid segregation and transitoryderepression of pilus synthesis. As predicted by the theory, mating experiments with the plasmid Rl in Eschericlriacoli K12 demonstrated that the estimate y is unaffected by cell density, donocrecipient ratio and mating time.Unlike previous techniques, our method allows us to investigate the effect of environmental factors on plasmidtransfer rates when these factors also influence population growth rates. To illustrate this, we examined the effectof temperature on the rate of plasmid transfer.

U2 - 10.1099/00221287-136-11-2319

DO - 10.1099/00221287-136-11-2319

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VL - 136

JO - Journal of General Microbiology

JF - Journal of General Microbiology

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