Electrochemical sensing of biomarker for diagnostics of bacteria-specific infections

Fatima AlZahraa Alatraktchi, Helle Krogh Johansen, Søren Molin, Winnie Edith Svendsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review


Aim: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin. Materials & methods: Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions. Results & conclusion: Pyocyanin was detected at concentrations down to 125 nM in a 50 μM mixture of interfering compounds with a reproducibility of r 2 = 0.999 (n = 5) within 200 s. The results document a step toward a point-of-care technique for diagnosis of P. aeruginosa infections.

TidsskriftNanomedicine: Nanotechnology, Biology and Medicine
Udgave nummer16
Sider (fra-til)2185-2195
Antal sider11
StatusUdgivet - 2016
Udgivet eksterntJa

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