Effects, Uptake, and Depuration Kinetics of Silver Oxide and Copper Oxide Nanoparticles in a Marine Deposit Feeder, Macoma balthica

Lina Dai, Kristian Syberg, Gary Thomas Banta, Henriette Selck, Valery E. Forbes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Ag and CuO engineered nanoparticles (ENPs) have wide applications in industry and commercial products and may be released from wastewater into the aquatic environment. Limited information is currently available on metal ENP effects, uptake, and depuration kinetics in aquatic organisms. In the present study, a deposit-feeding clam, Macoma balthica, was exposed to sediment spiked with Ag and Cu in different forms (aqueous ions, nanoparticles, and micrometer-sized particles) in three experiments. In all experiments, no effects on mortality, condition index, or burrowing behavior were observed for any of the metal forms at measured sediment concentrations (150−200 μg/g) during 35 d of exposure. No genotoxicity was observed following exposure, measured as DNA damage with the single-cell gel electrophoresis assay (comet assay). Bioaccumulation of both Ag and Cu in the clams was form dependent such that bioaccumulation from sediment spiked with aqueous ions > nanoparticles > micrometer-sized particles. Cu uptake and depuration kinetics were studied in more detail yielding net uptake rates (μg Cu/g dw soft tissue/d) in soft tissue of 0.640, 0.464, and 0.091 for sediment spiked with aqueous Cu ions, CuO nanoparticle,s and micrometer-sized CuO particles, respectively, supporting that net uptake was dependent on form. Depuration rate constants (d−1) from soft tissue were −0.074, −0.030, and 0.019 for Cu added to sediment as aqueous Cu ions, CuO
nanoparticles, and micrometer-sized CuO particles, respectively. Ensuring sustainable use of nanotechnology requires the development of better methods for detecting and quantifying ENPs, particularly in sediment.

OriginalsprogEngelsk
TidsskriftACS Sustainable Chemistry & Engineering
Vol/bind1
Udgave nummer7
Sider (fra-til)760-767
Antal sider8
ISSN2168-0485
DOI
StatusUdgivet - 2013

Emneord

  • nanoparticle
  • økotoksikologi
  • bioakkumulering

Citer dette

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title = "Effects, Uptake, and Depuration Kinetics of Silver Oxide and Copper Oxide Nanoparticles in a Marine Deposit Feeder, Macoma balthica",
abstract = "Ag and CuO engineered nanoparticles (ENPs) have wide applications in industry and commercial products and may be released from wastewater into the aquatic environment. Limited information is currently available on metal ENP effects, uptake, and depuration kinetics in aquatic organisms. In the present study, a deposit-feeding clam, Macoma balthica, was exposed to sediment spiked with Ag and Cu in different forms (aqueous ions, nanoparticles, and micrometer-sized particles) in three experiments. In all experiments, no effects on mortality, condition index, or burrowing behavior were observed for any of the metal forms at measured sediment concentrations (150−200 μg/g) during 35 d of exposure. No genotoxicity was observed following exposure, measured as DNA damage with the single-cell gel electrophoresis assay (comet assay). Bioaccumulation of both Ag and Cu in the clams was form dependent such that bioaccumulation from sediment spiked with aqueous ions > nanoparticles > micrometer-sized particles. Cu uptake and depuration kinetics were studied in more detail yielding net uptake rates (μg Cu/g dw soft tissue/d) in soft tissue of 0.640, 0.464, and 0.091 for sediment spiked with aqueous Cu ions, CuO nanoparticle,s and micrometer-sized CuO particles, respectively, supporting that net uptake was dependent on form. Depuration rate constants (d−1) from soft tissue were −0.074, −0.030, and 0.019 for Cu added to sediment as aqueous Cu ions, CuO nanoparticles, and micrometer-sized CuO particles, respectively. Ensuring sustainable use of nanotechnology requires the development of better methods for detecting and quantifying ENPs, particularly in sediment.",
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author = "Lina Dai and Kristian Syberg and Banta, {Gary Thomas} and Henriette Selck and Forbes, {Valery E.}",
year = "2013",
doi = "10.1021/sc4000434",
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volume = "1",
pages = "760--767",
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Effects, Uptake, and Depuration Kinetics of Silver Oxide and Copper Oxide Nanoparticles in a Marine Deposit Feeder, Macoma balthica. / Dai, Lina; Syberg, Kristian; Banta, Gary Thomas; Selck, Henriette; Forbes, Valery E.

I: ACS Sustainable Chemistry & Engineering, Bind 1, Nr. 7, 2013, s. 760-767.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Effects, Uptake, and Depuration Kinetics of Silver Oxide and Copper Oxide Nanoparticles in a Marine Deposit Feeder, Macoma balthica

AU - Dai, Lina

AU - Syberg, Kristian

AU - Banta, Gary Thomas

AU - Selck, Henriette

AU - Forbes, Valery E.

PY - 2013

Y1 - 2013

N2 - Ag and CuO engineered nanoparticles (ENPs) have wide applications in industry and commercial products and may be released from wastewater into the aquatic environment. Limited information is currently available on metal ENP effects, uptake, and depuration kinetics in aquatic organisms. In the present study, a deposit-feeding clam, Macoma balthica, was exposed to sediment spiked with Ag and Cu in different forms (aqueous ions, nanoparticles, and micrometer-sized particles) in three experiments. In all experiments, no effects on mortality, condition index, or burrowing behavior were observed for any of the metal forms at measured sediment concentrations (150−200 μg/g) during 35 d of exposure. No genotoxicity was observed following exposure, measured as DNA damage with the single-cell gel electrophoresis assay (comet assay). Bioaccumulation of both Ag and Cu in the clams was form dependent such that bioaccumulation from sediment spiked with aqueous ions > nanoparticles > micrometer-sized particles. Cu uptake and depuration kinetics were studied in more detail yielding net uptake rates (μg Cu/g dw soft tissue/d) in soft tissue of 0.640, 0.464, and 0.091 for sediment spiked with aqueous Cu ions, CuO nanoparticle,s and micrometer-sized CuO particles, respectively, supporting that net uptake was dependent on form. Depuration rate constants (d−1) from soft tissue were −0.074, −0.030, and 0.019 for Cu added to sediment as aqueous Cu ions, CuO nanoparticles, and micrometer-sized CuO particles, respectively. Ensuring sustainable use of nanotechnology requires the development of better methods for detecting and quantifying ENPs, particularly in sediment.

AB - Ag and CuO engineered nanoparticles (ENPs) have wide applications in industry and commercial products and may be released from wastewater into the aquatic environment. Limited information is currently available on metal ENP effects, uptake, and depuration kinetics in aquatic organisms. In the present study, a deposit-feeding clam, Macoma balthica, was exposed to sediment spiked with Ag and Cu in different forms (aqueous ions, nanoparticles, and micrometer-sized particles) in three experiments. In all experiments, no effects on mortality, condition index, or burrowing behavior were observed for any of the metal forms at measured sediment concentrations (150−200 μg/g) during 35 d of exposure. No genotoxicity was observed following exposure, measured as DNA damage with the single-cell gel electrophoresis assay (comet assay). Bioaccumulation of both Ag and Cu in the clams was form dependent such that bioaccumulation from sediment spiked with aqueous ions > nanoparticles > micrometer-sized particles. Cu uptake and depuration kinetics were studied in more detail yielding net uptake rates (μg Cu/g dw soft tissue/d) in soft tissue of 0.640, 0.464, and 0.091 for sediment spiked with aqueous Cu ions, CuO nanoparticle,s and micrometer-sized CuO particles, respectively, supporting that net uptake was dependent on form. Depuration rate constants (d−1) from soft tissue were −0.074, −0.030, and 0.019 for Cu added to sediment as aqueous Cu ions, CuO nanoparticles, and micrometer-sized CuO particles, respectively. Ensuring sustainable use of nanotechnology requires the development of better methods for detecting and quantifying ENPs, particularly in sediment.

KW - nanoparticle

KW - økotoksikologi

KW - bioakkumulering

U2 - 10.1021/sc4000434

DO - 10.1021/sc4000434

M3 - Journal article

VL - 1

SP - 760

EP - 767

JO - A C S Sustainable Chemistry & Engineering

JF - A C S Sustainable Chemistry & Engineering

SN - 2168-0485

IS - 7

ER -