Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: a model system for cellulase-poly-phenol interactions

Søren Nymand Olsen, Christina Helena Bohlin, Leigh Murphy, Kim Borch, K.C. McFarland, Matt D. Sweeney, Peter Westh

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS–lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In the current work we address this hypothesis using tannic acid (TAN) as a general poly-phenolic model compound (for lignin and soluble phenolics) and measure the mutual interactions of cellulases (CBHI, CBHII, EGI, EGII and BG), TAN and NIS (Triton X-100) using isothermal titration calorimetry (ITC). The experimental results suggest rather strong enzyme-specific interactions with TAN in reasonable agreement with enzyme specific lignin inhibition found in the literature. Enzyme–TAN interactions were disrupted by the presence of NIS by a mechanism of strong TAN–NIS interaction. The presence of NIS also alleviated the inhibitory effect of TAN on cellulase activity. All together the current work provides strong indications that favorable NIS–poly-phenol interactions alleviate non-productive cellulase–poly-phenol interactions and hence may provide a mechanism for the accelerating effect of NIS on lignocellulose hydrolysis.

OriginalsprogEngelsk
TidsskriftEnzyme and Microbial Technology
Vol/bind49
Udgave nummer4
Sider (fra-til)353-359
ISSN0141-0229
DOI
StatusUdgivet - 2011

Emneord

  • Cellulase activity
  • Non-ionic surfactants
  • Poly-phenol
  • Tannic acid
  • Isothermal titration calorimetry

Citer dette

Olsen, Søren Nymand ; Bohlin, Christina Helena ; Murphy, Leigh ; Borch, Kim ; McFarland, K.C. ; Sweeney, Matt D. ; Westh, Peter. / Effects of non-ionic surfactants on the interactions between cellulases and tannic acid : a model system for cellulase-poly-phenol interactions. I: Enzyme and Microbial Technology. 2011 ; Bind 49, Nr. 4. s. 353-359.
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abstract = "Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS–lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In the current work we address this hypothesis using tannic acid (TAN) as a general poly-phenolic model compound (for lignin and soluble phenolics) and measure the mutual interactions of cellulases (CBHI, CBHII, EGI, EGII and BG), TAN and NIS (Triton X-100) using isothermal titration calorimetry (ITC). The experimental results suggest rather strong enzyme-specific interactions with TAN in reasonable agreement with enzyme specific lignin inhibition found in the literature. Enzyme–TAN interactions were disrupted by the presence of NIS by a mechanism of strong TAN–NIS interaction. The presence of NIS also alleviated the inhibitory effect of TAN on cellulase activity. All together the current work provides strong indications that favorable NIS–poly-phenol interactions alleviate non-productive cellulase–poly-phenol interactions and hence may provide a mechanism for the accelerating effect of NIS on lignocellulose hydrolysis.",
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Effects of non-ionic surfactants on the interactions between cellulases and tannic acid : a model system for cellulase-poly-phenol interactions. / Olsen, Søren Nymand; Bohlin, Christina Helena; Murphy, Leigh; Borch, Kim; McFarland, K.C.; Sweeney, Matt D.; Westh, Peter.

I: Enzyme and Microbial Technology, Bind 49, Nr. 4, 2011, s. 353-359.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Effects of non-ionic surfactants on the interactions between cellulases and tannic acid

T2 - a model system for cellulase-poly-phenol interactions

AU - Olsen, Søren Nymand

AU - Bohlin, Christina Helena

AU - Murphy, Leigh

AU - Borch, Kim

AU - McFarland, K.C.

AU - Sweeney, Matt D.

AU - Westh, Peter

PY - 2011

Y1 - 2011

N2 - Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS–lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In the current work we address this hypothesis using tannic acid (TAN) as a general poly-phenolic model compound (for lignin and soluble phenolics) and measure the mutual interactions of cellulases (CBHI, CBHII, EGI, EGII and BG), TAN and NIS (Triton X-100) using isothermal titration calorimetry (ITC). The experimental results suggest rather strong enzyme-specific interactions with TAN in reasonable agreement with enzyme specific lignin inhibition found in the literature. Enzyme–TAN interactions were disrupted by the presence of NIS by a mechanism of strong TAN–NIS interaction. The presence of NIS also alleviated the inhibitory effect of TAN on cellulase activity. All together the current work provides strong indications that favorable NIS–poly-phenol interactions alleviate non-productive cellulase–poly-phenol interactions and hence may provide a mechanism for the accelerating effect of NIS on lignocellulose hydrolysis.

AB - Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS–lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In the current work we address this hypothesis using tannic acid (TAN) as a general poly-phenolic model compound (for lignin and soluble phenolics) and measure the mutual interactions of cellulases (CBHI, CBHII, EGI, EGII and BG), TAN and NIS (Triton X-100) using isothermal titration calorimetry (ITC). The experimental results suggest rather strong enzyme-specific interactions with TAN in reasonable agreement with enzyme specific lignin inhibition found in the literature. Enzyme–TAN interactions were disrupted by the presence of NIS by a mechanism of strong TAN–NIS interaction. The presence of NIS also alleviated the inhibitory effect of TAN on cellulase activity. All together the current work provides strong indications that favorable NIS–poly-phenol interactions alleviate non-productive cellulase–poly-phenol interactions and hence may provide a mechanism for the accelerating effect of NIS on lignocellulose hydrolysis.

KW - Cellulase activity

KW - Non-ionic surfactants

KW - Poly-phenol

KW - Tannic acid

KW - Isothermal titration calorimetry

U2 - 10.1016/j.enzmictec.2011.06.015

DO - 10.1016/j.enzmictec.2011.06.015

M3 - Journal article

VL - 49

SP - 353

EP - 359

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 4

ER -