Effects of combined exposures of fluoranthene and polyethylene or polyhydroxybutyrate microplastics on oxidative stress biomarkers in the blue mussel (Mytilus edulis)

Gabriele Magara, Farhan Khan, Marika Pinti, Kristian Syberg, Angelo Inzirillo, Antonia Concetta Elia

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

A growing interest in developing and commercialization of new eco-friendly plastic polymers is occurring attributed to the impact of marine plastics debris and microplastics that result from the degradation of oil-based polymers as these substances adversely affect ecosystem health. Recently, polyhydroxybutyrate (PHB) has become of interest due to its biodegradability and physicochemical properties. However, biological consequences resulting from bioplastics exposure remain to be determined. Further, few data are apparently available regarding the potential for bioplastics to act as a vector for exogenous chemicals in the environment. The aim of the study was to compare the effects of polyethylene (PE MPs) and polyhydroxybutyrate (PHB MPs) microplastics administered alone or in combination with fluoranthene (Flu) on detoxifying enzymes in digestive glands and gills of Mytilus edulis. Blue mussels were exposed for 96h to eight experimental groups: control, Flu-only, PE MPs-only, PHB MPs-only, PE MPs-Flu co-exposure, PHB MPs-Flu co-exposure, Flu-incubated PE MPs, and Flu-incubated PHB MPs. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), glutathione S-transferase (GST), and glutathione reductase (GR) were found to be significantly susceptible to Flu and plastics in both tissues. Interestingly, a single exposure to PHB MPs led to decreased activity levels of CAT and GST in gills, SOD in digestive glands and SeGPx in both tissues. In co-exposure and incubation treatments, biochemical responses were generally comparable with those exerted by PE MPs or PHB MPs only, suggesting an apparent absence of combined effects of microplastics with the pollutant. Data demonstrated the ecotoxicological impact of bioplastics materials on digestive glands and gills of Mytilus edulis.
OriginalsprogEngelsk
TidsskriftJournal of Toxicology and Environmental Health. Part A
Vol/bind82
Udgave nummer10
Sider (fra-til)616-625
Antal sider10
ISSN1528-7394
DOI
StatusUdgivet - 2019

Bibliografisk note

Important note from the Publisher regarding the attached document: “This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Toxicology and Environmental Health. Part A on 24 Jun 2019, available online: http://www.tandfonline.com/10.1080/15287394.2019.1633451.”

Citer dette

@article{38e05ce235354de787b2e95145e1d3fd,
title = "Effects of combined exposures of fluoranthene and polyethylene or polyhydroxybutyrate microplastics on oxidative stress biomarkers in the blue mussel (Mytilus edulis)",
abstract = "A growing interest in developing and commercialization of new eco-friendly plastic polymers is occurring attributed to the impact of marine plastics debris and microplastics that result from the degradation of oil-based polymers as these substances adversely affect ecosystem health. Recently, polyhydroxybutyrate (PHB) has become of interest due to its biodegradability and physicochemical properties. However, biological consequences resulting from bioplastics exposure remain to be determined. Further, few data are apparently available regarding the potential for bioplastics to act as a vector for exogenous chemicals in the environment. The aim of the study was to compare the effects of polyethylene (PE MPs) and polyhydroxybutyrate (PHB MPs) microplastics administered alone or in combination with fluoranthene (Flu) on detoxifying enzymes in digestive glands and gills of Mytilus edulis. Blue mussels were exposed for 96h to eight experimental groups: control, Flu-only, PE MPs-only, PHB MPs-only, PE MPs-Flu co-exposure, PHB MPs-Flu co-exposure, Flu-incubated PE MPs, and Flu-incubated PHB MPs. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), glutathione S-transferase (GST), and glutathione reductase (GR) were found to be significantly susceptible to Flu and plastics in both tissues. Interestingly, a single exposure to PHB MPs led to decreased activity levels of CAT and GST in gills, SOD in digestive glands and SeGPx in both tissues. In co-exposure and incubation treatments, biochemical responses were generally comparable with those exerted by PE MPs or PHB MPs only, suggesting an apparent absence of combined effects of microplastics with the pollutant. Data demonstrated the ecotoxicological impact of bioplastics materials on digestive glands and gills of Mytilus edulis.",
author = "Gabriele Magara and Farhan Khan and Marika Pinti and Kristian Syberg and Angelo Inzirillo and Elia, {Antonia Concetta}",
note = "Important note from the Publisher regarding the attached document: “This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Toxicology and Environmental Health. Part A on 24 Jun 2019, available online: http://www.tandfonline.com/10.1080/15287394.2019.1633451.”",
year = "2019",
doi = "10.1080/15287394.2019.1633451",
language = "English",
volume = "82",
pages = "616--625",
journal = "Journal of Toxicology and Environmental Health. Part A: Current Issues",
issn = "1528-7394",
publisher = "Taylor & Francis",
number = "10",

}

Effects of combined exposures of fluoranthene and polyethylene or polyhydroxybutyrate microplastics on oxidative stress biomarkers in the blue mussel (Mytilus edulis). / Magara, Gabriele; Khan, Farhan; Pinti, Marika ; Syberg, Kristian; Inzirillo, Angelo ; Elia, Antonia Concetta .

I: Journal of Toxicology and Environmental Health. Part A, Bind 82, Nr. 10, 2019, s. 616-625.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Effects of combined exposures of fluoranthene and polyethylene or polyhydroxybutyrate microplastics on oxidative stress biomarkers in the blue mussel (Mytilus edulis)

AU - Magara, Gabriele

AU - Khan, Farhan

AU - Pinti, Marika

AU - Syberg, Kristian

AU - Inzirillo, Angelo

AU - Elia, Antonia Concetta

N1 - Important note from the Publisher regarding the attached document: “This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Toxicology and Environmental Health. Part A on 24 Jun 2019, available online: http://www.tandfonline.com/10.1080/15287394.2019.1633451.”

PY - 2019

Y1 - 2019

N2 - A growing interest in developing and commercialization of new eco-friendly plastic polymers is occurring attributed to the impact of marine plastics debris and microplastics that result from the degradation of oil-based polymers as these substances adversely affect ecosystem health. Recently, polyhydroxybutyrate (PHB) has become of interest due to its biodegradability and physicochemical properties. However, biological consequences resulting from bioplastics exposure remain to be determined. Further, few data are apparently available regarding the potential for bioplastics to act as a vector for exogenous chemicals in the environment. The aim of the study was to compare the effects of polyethylene (PE MPs) and polyhydroxybutyrate (PHB MPs) microplastics administered alone or in combination with fluoranthene (Flu) on detoxifying enzymes in digestive glands and gills of Mytilus edulis. Blue mussels were exposed for 96h to eight experimental groups: control, Flu-only, PE MPs-only, PHB MPs-only, PE MPs-Flu co-exposure, PHB MPs-Flu co-exposure, Flu-incubated PE MPs, and Flu-incubated PHB MPs. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), glutathione S-transferase (GST), and glutathione reductase (GR) were found to be significantly susceptible to Flu and plastics in both tissues. Interestingly, a single exposure to PHB MPs led to decreased activity levels of CAT and GST in gills, SOD in digestive glands and SeGPx in both tissues. In co-exposure and incubation treatments, biochemical responses were generally comparable with those exerted by PE MPs or PHB MPs only, suggesting an apparent absence of combined effects of microplastics with the pollutant. Data demonstrated the ecotoxicological impact of bioplastics materials on digestive glands and gills of Mytilus edulis.

AB - A growing interest in developing and commercialization of new eco-friendly plastic polymers is occurring attributed to the impact of marine plastics debris and microplastics that result from the degradation of oil-based polymers as these substances adversely affect ecosystem health. Recently, polyhydroxybutyrate (PHB) has become of interest due to its biodegradability and physicochemical properties. However, biological consequences resulting from bioplastics exposure remain to be determined. Further, few data are apparently available regarding the potential for bioplastics to act as a vector for exogenous chemicals in the environment. The aim of the study was to compare the effects of polyethylene (PE MPs) and polyhydroxybutyrate (PHB MPs) microplastics administered alone or in combination with fluoranthene (Flu) on detoxifying enzymes in digestive glands and gills of Mytilus edulis. Blue mussels were exposed for 96h to eight experimental groups: control, Flu-only, PE MPs-only, PHB MPs-only, PE MPs-Flu co-exposure, PHB MPs-Flu co-exposure, Flu-incubated PE MPs, and Flu-incubated PHB MPs. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), glutathione S-transferase (GST), and glutathione reductase (GR) were found to be significantly susceptible to Flu and plastics in both tissues. Interestingly, a single exposure to PHB MPs led to decreased activity levels of CAT and GST in gills, SOD in digestive glands and SeGPx in both tissues. In co-exposure and incubation treatments, biochemical responses were generally comparable with those exerted by PE MPs or PHB MPs only, suggesting an apparent absence of combined effects of microplastics with the pollutant. Data demonstrated the ecotoxicological impact of bioplastics materials on digestive glands and gills of Mytilus edulis.

U2 - 10.1080/15287394.2019.1633451

DO - 10.1080/15287394.2019.1633451

M3 - Journal article

VL - 82

SP - 616

EP - 625

JO - Journal of Toxicology and Environmental Health. Part A: Current Issues

JF - Journal of Toxicology and Environmental Health. Part A: Current Issues

SN - 1528-7394

IS - 10

ER -