Abstract
Consumption of cruciferous vegetables and cancer prevention seem to be positively associated. We present an easy two-step synthesis for 4-methoxyindole-3-carbinol (4MeOI3C), the expected breakdown product of 4-methoxyglucobrassicin during ingestion. 4MeOI3C inhibited the proliferation of human colon cancer cells DLD-1 and HCT 116 with IC(50) values of 116 microM and 96 microM, respectively, after 48 h in vitro, and is therefore a more potent inhibitor than indole-3-carbinol (I3C). 4MeOI3C and I3C combined in different molar ratios inhibited proliferation in a nearly additive to slightly synergistic manner. Proliferation was inhibited by 100 microM 4MeOI3C after 48 h without affecting cell cycle phase distribution, indicating an overall-slowdown effect on the cell cycle. However, 200 microM 4MeOI3C caused a very high level of cell death and an accumulation of living cells in the G(0)/G(1) phase, indicating a concentration-dependent mode of action. We conclude that 4MeOI3C might play a role in the cancer preventive effect of cruciferous vegetables.
Originalsprog | Engelsk |
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Tidsskrift | Journal of Agricultural and Food Chemistry |
Vol/bind | 58 |
Udgave nummer | 14 |
Sider (fra-til) | 8453-8459 |
ISSN | 0021-8561 |
DOI | |
Status | Udgivet - 28 jul. 2010 |
Emneord
- 4-methoxyindole-3-carbinol
- chemoprevention
- indole-3-carbinol