Effect of 4-methoxyindole-3-carbinol on the proliferation of colon cancer cells in vitro, when treated alone or in combination with indole-3-carbinol

Remy Kronbak, Fritz Duus, Ole Vang

Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review

Abstract

Consumption of cruciferous vegetables and cancer prevention seem to be positively associated. We present an easy two-step synthesis for 4-methoxyindole-3-carbinol (4MeOI3C), the expected breakdown product of 4-methoxyglucobrassicin during ingestion. 4MeOI3C inhibited the proliferation of human colon cancer cells DLD-1 and HCT 116 with IC(50) values of 116 microM and 96 microM, respectively, after 48 h in vitro, and is therefore a more potent inhibitor than indole-3-carbinol (I3C). 4MeOI3C and I3C combined in different molar ratios inhibited proliferation in a nearly additive to slightly synergistic manner. Proliferation was inhibited by 100 microM 4MeOI3C after 48 h without affecting cell cycle phase distribution, indicating an overall-slowdown effect on the cell cycle. However, 200 microM 4MeOI3C caused a very high level of cell death and an accumulation of living cells in the G(0)/G(1) phase, indicating a concentration-dependent mode of action. We conclude that 4MeOI3C might play a role in the cancer preventive effect of cruciferous vegetables.
OriginalsprogEngelsk
TidsskriftJournal of Agricultural and Food Chemistry
Vol/bind58
Udgave nummer14
Sider (fra-til)8453-8459
ISSN0021-8561
DOI
StatusUdgivet - 28 jul. 2010

Emneord

  • 4-methoxyindole-3-carbinol
  • chemoprevention
  • indole-3-carbinol

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