Titanium (Ti) is a widely used material for surgical implants; total joint replacements (TJRs), screws and plates for fixing bones and dental implants are forged from Ti. Whilst Ti integrates well into host tissue approximately 10% of TJRs will fail in the lifetime of the patient through a process known as aseptic loosening. These failures necessitate revision arthroplasties which are more complicated and costly than the initial procedure. Finding ways of enhancing early (osseo)integration of TJRs is therefore highly desirable and continues to represent a research priority in current biomaterial design. One way of realising improvements in implant quality is to coat the Ti surface with small biological agents known to support human osteoblast formation and maturation at Ti surfaces. Lysophosphatidic acid (LPA) and certain LPA analogues offer potential solutions as Ti coatings in reducing aseptic loosening. Herein we present evidence for the successful bio-functionalisation of Ti using LPA. This modified Ti surface heightened the maturation of human osteoblasts, as supported by increased expression of alkaline phosphatase. These functionalised surfaces also deterred the attachment and growth of Staphylococcus aureus, a bacterium often associated with implant failures through sepsis. Collectively we provide evidence for the fabrication of a dual-action Ti surface finish, a highly desirable feature towards the development of next-generation implantable devices.
Bibliografisk noteKorrektion 21-1-2016: Dr. Jianxing Zhang should be included in the author byline. He should be listed as the fourth author, and his affiliation #6: Department of Medicinal Chemistry, The University of Utah, Salt Lake City, Utah, United States of America. The contributions of this author are as follows: Conception the design of the work, acquisition and analysis/interpretation of data, synthesized the molecules employed in the experiments, contributed to reagents/materials/analysis tools, drafting as well as revising of the manuscript, and final approval of the published version.
The correction citation is: Skindersoe ME, Krogfelt KA, Blom A, Zhang J, Jiang G, Prestwich GD, et al. (2015) Dual Action of Lysophosphatidate-Functionalised Titanium: Interactions with Human (MG63) Osteoblasts and Methicillin Resistant Staphylococcus aureus. PLoS ONE 10(11): e0143509. doi:10.1371/journal.pone.0143509