Developmental expression of Manduca shade, the P450 mediating the final step in molting hormone synthesis.

Kim Rewitz, Robert Rybczynski, James T. Warren, Lawrence I. Gilbert

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Udgivelsesdato: Marts
    OriginalsprogEngelsk
    TidsskriftMolecular and Cellular Endocrinology
    Vol/bind247
    Udgave nummer1-2
    Sider (fra-til)166-174
    Antal sider8
    ISSN0303-7207
    StatusUdgivet - 2006

    Citer dette

    Rewitz, Kim ; Rybczynski, Robert ; Warren, James T. ; Gilbert, Lawrence I. / Developmental expression of Manduca shade, the P450 mediating the final step in molting hormone synthesis. I: Molecular and Cellular Endocrinology. 2006 ; Bind 247, Nr. 1-2. s. 166-174.
    @article{5fdc38a0d23111db9c7c000ea68e967b,
    title = "Developmental expression of Manduca shade, the P450 mediating the final step in molting hormone synthesis.",
    abstract = "The ecdysone 20-monooxygenase (E20MO; 20-hydroxylase) is the enzyme that mediates the conversion of ecdysone (E) to the active insect molting hormone, 20-hydroxyecdysone (20E), which coordinates developmental progression. We report the identification and developmental expression of the Halloween gene shade (shd; CYP314A1) that encodes the E20MO in the tobacco hornworm, Manduca sexta. Manduca Shd (MsShd) mediates the conversion of E to 20E when expressed in Drosophila S2 cells. In accord with the central dogma, the data show that Msshd is expressed mainly in the midgut, Malpighian tubules, fat body and epidermis with very low expression in the prothoracic gland and nervous system. Developmental variations in E20MO enzymatic activity are almost perfectly correlated with comparable changes in the gene expression of Msshd in the fat body and midgut during the fifth instar and the beginning of pupal-adult development. The results indicate three successive and overlapping peaks of expression in the fat body, midgut and Malpighian tubules, respectively, during the fifth larval instar. The data suggest that precise tissue-specific transcriptional regulation controls the levels, and thereby the activity, of the Manduca E20MO.",
    keywords = "CYP314A1, 20-hydroxyecdysone, Molting, Development, Insect, Steroid hydroxylation",
    author = "Kim Rewitz and Robert Rybczynski and Warren, {James T.} and Gilbert, {Lawrence I.}",
    year = "2006",
    language = "English",
    volume = "247",
    pages = "166--174",
    journal = "Molecular and Cellular Endocrinology",
    issn = "0303-7207",
    publisher = "Elsevier Ireland Ltd",
    number = "1-2",

    }

    Developmental expression of Manduca shade, the P450 mediating the final step in molting hormone synthesis. / Rewitz, Kim; Rybczynski, Robert; Warren, James T.; Gilbert, Lawrence I.

    I: Molecular and Cellular Endocrinology, Bind 247, Nr. 1-2, 2006, s. 166-174.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Developmental expression of Manduca shade, the P450 mediating the final step in molting hormone synthesis.

    AU - Rewitz, Kim

    AU - Rybczynski, Robert

    AU - Warren, James T.

    AU - Gilbert, Lawrence I.

    PY - 2006

    Y1 - 2006

    N2 - The ecdysone 20-monooxygenase (E20MO; 20-hydroxylase) is the enzyme that mediates the conversion of ecdysone (E) to the active insect molting hormone, 20-hydroxyecdysone (20E), which coordinates developmental progression. We report the identification and developmental expression of the Halloween gene shade (shd; CYP314A1) that encodes the E20MO in the tobacco hornworm, Manduca sexta. Manduca Shd (MsShd) mediates the conversion of E to 20E when expressed in Drosophila S2 cells. In accord with the central dogma, the data show that Msshd is expressed mainly in the midgut, Malpighian tubules, fat body and epidermis with very low expression in the prothoracic gland and nervous system. Developmental variations in E20MO enzymatic activity are almost perfectly correlated with comparable changes in the gene expression of Msshd in the fat body and midgut during the fifth instar and the beginning of pupal-adult development. The results indicate three successive and overlapping peaks of expression in the fat body, midgut and Malpighian tubules, respectively, during the fifth larval instar. The data suggest that precise tissue-specific transcriptional regulation controls the levels, and thereby the activity, of the Manduca E20MO.

    AB - The ecdysone 20-monooxygenase (E20MO; 20-hydroxylase) is the enzyme that mediates the conversion of ecdysone (E) to the active insect molting hormone, 20-hydroxyecdysone (20E), which coordinates developmental progression. We report the identification and developmental expression of the Halloween gene shade (shd; CYP314A1) that encodes the E20MO in the tobacco hornworm, Manduca sexta. Manduca Shd (MsShd) mediates the conversion of E to 20E when expressed in Drosophila S2 cells. In accord with the central dogma, the data show that Msshd is expressed mainly in the midgut, Malpighian tubules, fat body and epidermis with very low expression in the prothoracic gland and nervous system. Developmental variations in E20MO enzymatic activity are almost perfectly correlated with comparable changes in the gene expression of Msshd in the fat body and midgut during the fifth instar and the beginning of pupal-adult development. The results indicate three successive and overlapping peaks of expression in the fat body, midgut and Malpighian tubules, respectively, during the fifth larval instar. The data suggest that precise tissue-specific transcriptional regulation controls the levels, and thereby the activity, of the Manduca E20MO.

    KW - CYP314A1

    KW - 20-hydroxyecdysone

    KW - Molting

    KW - Development

    KW - Insect

    KW - Steroid hydroxylation

    M3 - Journal article

    VL - 247

    SP - 166

    EP - 174

    JO - Molecular and Cellular Endocrinology

    JF - Molecular and Cellular Endocrinology

    SN - 0303-7207

    IS - 1-2

    ER -