Detection of salt bridges to lysines in solution in barnase

Poul Erik Hansen, Michael P. Williamson, Andrea M. Hounslow, Joe Ford, Kyle Fowler, Max Hebditch

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstract

We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.
OriginalsprogEngelsk
TidsskriftChemical Communications
Vol/bind49
Sider (fra-til)9824-9826
Antal sider3
ISSN1359-7345
DOI
StatusUdgivet - 2013

Emneord

  • lysines
  • salt bridges
  • isotope effects
  • barnase
  • proteins

Citer dette

Hansen, P. E., Williamson, M. P., Hounslow, A. M., Ford, J., Fowler, K., & Hebditch, M. (2013). Detection of salt bridges to lysines in solution in barnase. Chemical Communications, 49, 9824-9826. https://doi.org/10.1039/C3CC45602A